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. Author manuscript; available in PMC: 2024 Dec 9.
Published in final edited form as: Traffic. 2007 Apr 25;8(6):702–17. doi: 10.1111/j.1600-0854.2007.00565.x

Figure 3. Overexpression of CRIB domain of N-WASP inhibits endocytosis via GEEC pathway.

Figure 3

A) FRαTb cells transfected with GFP-CRIB (green; top panel) or full-length GFP-N-WASP (green; bottom panel) were pulsed with R-Dex (red), A647-Tf (blue) for 5 min at 37°C, and imaged at low magnification using a × 20, 0.75 NA objective. Note GFP-CRIB but not GFP-N-WASP-transfected cells show a reduction in fluid-phase uptake relative to non-transfected cells. Scale bar, 5 μm. B) Histogram shows the extent of uptake in a 5-min pulse of indicated endocytic tracers in cells overexpressing GFP-CRIB or GFP-N-WASP, relative to that in non-transfected cells (value of controls set to 1). Values represented are wt. mean ± SEM obtained across two independent experiments.