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. Author manuscript; available in PMC: 2024 Dec 9.
Published in final edited form as: Traffic. 2007 Apr 25;8(6):702–17. doi: 10.1111/j.1600-0854.2007.00565.x

Figure 4. Nascent endocytic carriers co-localize with Cdc42, N-WASP and actin.

Figure 4

FRαTb cells expressing GFP-Cdc42 (B and G), GFP-N-WASP (C), actin–GFP (D), GFP-CRIB (E) or GFP-Cdc42-L61 (F) were pulsed with TMR-dextran (R-Dex; A–F) or A568-Tf (G) or A488-Tf (A) for 20 s at 37°C and rapidly washed, fixed and imaged under TIRF illumination (depth ∼100 nm). (H) Histogram shows the extent of co-localization of endosomes labelled with TMR-dextran (R-Dex) or A-Tf (TfR) with expressed GFP-tagged constructs, GFP-Cdc42 (WT), GFP-N-WASP (N-WASP), actin–GFP (actin), GFP-CRIB (CRIB) or GFP-Cdc42-L61 (L61). Extent of non-specific co-localization using the procedures in Materials and Methods is ∼3%. Data were obtained from 10–15 cells, threee to five experiments and represented as wt. mean ± SEM. Scale bar, 10 μm.