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. Author manuscript; available in PMC: 2026 Sep 1.
Published in final edited form as: Biophys Rep (N Y). 2026 Aug 26;6(4):100283. doi: 10.1016/j.bpr.2026.100283

Figure 3. Ergosterol and saturated asymmetrical glycerophospholipids drive high membrane dipole potential.

Figure 3

(A, B) Representative emission maximum (A) and fluorescence intensity ratio (R) values (B) of di-8-ANEPPS from spectroscopic measurements in single and two-component LUVs made with POPC or SDPC with either ergosterol (Erg) or diplopterol (Dip). Values represent average ± S.D. (n = 3 biological repeats). p values were obtained by two-tailed unpaired parametric t-tests. (C) Representative single-plane fluorescence intensity ratio (R) maps (color-coded in a scale of 0.0-0.4) in S. pombe wild type and fasS.j. cells. (D) A plot representing the individual values of fluorescence intensity ratios (R) calculated at the cell tip regions of the plasma membrane and at the ER/NE in cells of specified genotypes. Representative regions of interest (ROIs) are shown in Supplemental Fig. S3. R values were obtained as described in Fig. 2. p values were obtained by two-tailed unpaired parametric t-tests, using the means of the three independent biological replicates (n = 3).