FIG 2.
Expression of the lmeA transcript and cellular levels of LM/LAM during stationary growth phase. (A) Relative expression of the lmeA transcript normalized to that of a housekeeping gene, gyrB. A significant increase in lmeA expression is noted by 72 h of growth in comparison to that at log phase (18 h). **, P < 0.05, by ANOVA. (B) SDS-PAGE analysis of LM/LAM during stationary phase. A representative image of a biological triplicate is shown. LAM decreased significantly more in the stationary phase than LM did. (C and D) Dot plots showing intensities of LM (C) and LAM (D) from biological replicates. Signal intensities were quantified using ImageQuant and normalized to the intensity of the WT at log phase. The averages are shown as horizontal bars. **, P < 0.01; *, P < 0.05, by ANOVA. ΔlmeA::C, ΔlmeA::PnativelmeA-HA strain (also see the text).