Sustained activation of C3aR in a human podocyte line impairs the morphological maturation of the cells

Abstract

The C3a receptor (C3aR) has been reported to be involved in various physiological and pathological processes, including the regulation of cellular structure development. Expression of C3aR has been reported in podocytes; however, data concerning the role of C3aR in podocyte morphology is scarce. The aim of the present study was to examine the effect of C3aR activation on the architectural development of podocytes. An immortal human podocyte line (HPC) was transfected with a C3a expression lentivirus vector or recombinant C3a. SB290157 was used to block the activation of C3aR. The expression of C3a in HPC cells was analyzed by reverse transcription-quantitative PCR (RT-qPCR) and ELISAs. Phase contrast and fluorescence microscopy were used to observe the morphology of the podocytes. The adhesive ability of HPC cells was analyzed using an attachment assay. RT-qPCR, cyto-immunofluorescence and western blotting were used to determine the expression levels of the adhesion-associated genes. The expression levels of carboxypeptidases in HPC cells was also detected by RT-qPCR. Compared with the untransfected and control virus-transfected HPC cells, the C3a-overexpressing cells (HPC-C3a) failed to expand their cell bodies and develop an arborized appearance in the process of maturation, which the control cells exhibited. In addition, HPC-C3a cells presented with decreased adhesive capacity, altered focal adhesion (FA) plaques and decreased expression of FA-associated genes. These effects were blocked by a C3aR antagonist; however, the addition of purified C3a could not completely mimic the effects of C3a overexpression. Furthermore, HPC cells expressed carboxypeptidases, which have been reported to be able to inactivate C3a. In summary, the results demonstrated that sustained C3aR activation impaired the morphological maturation of HPC cells, which may be associated with the altered expression of FA-associated genes and impaired FA. Since chronic complement activation has been reported in renal diseases, which indicate sustained C3aR activation in renal cells, including podocytes and podocyte progenitors, the possible role of C3aR in the dysregulation of podocyte architecture and podocyte regeneration requires further research.

Introduction

As a part of the immune system, the complement system is initially involved in host defense against pathogens. Notably, recent research has indicated that the complement system may also have certain non-immune functions and serve roles in physiological and pathological processes (1,2); for example, the complement system was discovered to be involved in the control of tissue morphogenesis, wound healing and synaptic pruning (1).

Human C3a anaphylatoxin (C3a), a C3 split fragment produced during the activation of the complement system, is one of the complement molecules that have been reported to serve non-immune roles. C3a was initially reported to be involved in the chemotaxis, recruitment and activation of inflammatory cells by binding to the C3a receptor (C3aR) on immune cells (3–5). However, C3aR has also been demonstrated to be expressed by certain non-immune cells, including in liver, kidney and nerve cells. Additionally, C3aR signaling has been revealed to participate in various physiological and pathological processes, including in the regulation of metabolism (6,7), histogenesis (8,9) and tissue repair (10). In the nervous system, C3aR was reported to be expressed by gliocytes, neural stem cells, neural progenitors and mature neurons (11–13), and was found to be involved in the regulation of neurogenesis and neural regeneration (14). Notably, C3aR signaling was demonstrated to be directly associated with the structure of neural development processes. Furthermore, C3aR has been implicated in axonal sprouting, neurite extension, synapsis formation and the recovery of injured neural structure and function (14,15).

Podocytes exhibit an octopus-like dendritic morphology consisting of a cell body, major processes and foot processes, similar to neural cells. Foot processes from neighboring podocytes interdigitate with each other, cover the outer side of the glomerular basement membrane and constitute one of the most important components of the glomerular filtration barrier. This unique architecture is the basis for podocyte function (16). Damage to podocyte structure, including foot process effacement, which is the retraction and flattening of foot processes, was confirmed to be directly associated with proteinuria in kidney diseases and the recovery of podocyte structure lead to remission (17,18). Despite the importance of the cellular structure in podocyte function, the mechanism underlying the management and regulation of podocyte architecture remains unclear.

C3aR expression in glomerular podocytes has been reported by several studies (19,20). However, data concerning the role of C3aR in podocytes is limited. Considering the morphological similarity between nerve cells and podocytes, and the role of C3aR in the neurogenesis, particularly in the regulation of neural morphology, we hypothesized that C3aR may serve a role in the development and management of podocyte architecture.

Derived from primarily cultured human glomerular podocytes, the conditionally immortal human podocyte line (HPC) mimics the process of podocyte architecture development. During the proliferation condition, the cells exhibit a typical cobblestone morphology. In the maturation condition, the cells undergo proliferation arrest, enlargement of the cell bodies and the development of the arborized form, with the formation of processes and a slit diaphragm complex, and the expression of podocyte-specific markers (21).

To investigate the possible role of C3aR signaling in the regulation of podocyte architecture development, the current study examined the effect of C3aR activation on the development of podocyte morphology by the overexpression of C3a in HPC cells and discussed the underlying molecular mechanism.

Materials and methods

Cell culture and stable overexpression of C3a in HPC cells

HPC was provided by Professor M.A. Saleem (Children's Renal Unit and Academic Renal Unit, University of Bristol) and cultured as previously described (21). Briefly, the cells were cultured in RPMI-1640 medium (Dalian Meilun Biology Technology Co., Ltd.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) and 1X Insulin-Transferrin-Selenium (Gibco; Thermo Fisher Scientific, Inc.) at 33°C for propagation (the proliferation condition) and at 37°C for differentiation (the maturation condition).

To obtain HPC cells stably overexpressing secretory C3a, the cells cultured in the proliferation condition were transfected with a recombinant lentivirus Lenti-C3a. The lentivirus was constructed based on a previous study (22), in which mouse secretory C3a was expressed by inserting the IL-6 signal peptide sequence prior to the C3a coding sequence. Similarly, a recombinant human C3a gene IL6 C3a containing the IL-6 signal peptide sequence (117–203 of NM_000600.3) and C3a coding sequence (2076–2306 of NM_000064.2) was synthesized. Following this, the expression vector pLenti6.3-IL6C3a-IRES2-EGFP was constructed by inserting the IL6C3a gene into the BamHI/AscI site of the pLenti6.3-MCS-IRES2-EGFP vector (Invitrogen; Thermo Fisher Scientific, Inc.). Finally, lentivirus Lenti-C3a was produced in 293T cells using the expression vector pLenti6.3-IL6C3a-IRES2-EGFP. Infection of HPC cells was performed when the cells cultured in the proliferation condition reached ~50% confluence.

At 2 days post-infection, the transfected cells were examined under a fluorescence microscope and >80% of the cells were found to have been transfected successfully. The cells were transferred to the selection medium containing 10 µg/ml blasticidin. Two weeks later, stably transfected HPC cells (HPC-C3a) were obtained. Vector pLenti6.3-MCS-IRES2-EGFP was directly used to construct the negative control (NC) lentivirus (Lenti-NC). Infection with Lenti-NC produced the HPC-NC cells. HPC-C3a cells cultured in medium containing 1 µM SB290157 (Merck KGaA) were used as the C3aR blocking control.

In the C3a stimulation tests, purified human C3a (Merck KGaA) was directly added to the cell culture medium once every two days at 37°C for a total of 6 days at a final concentration of 100 nM.

Cell morphology observation

To examine the morphological changes in the process of maturation, HPC cells (including untransfected HPC, HPC-NC, HPC-C3a and SB290157-treated HPC-C3a cells) were examined under phase contrast (Leica Microsystems GmbH; magnification, ×200) and fluorescence microscopes (Zeiss GmbH; magnification, ×400) following 0, 2, 7 and 14 days of being in the maturation condition. Given that normal HPC cells do not exhibit fluorescence, only the morphology of HPC-NC, HPC-C3a and HPC-C3a cells treated with SB290157 were visualized using fluorescence microscopy.

Cell adhesion assay

Cell adhesion assays were performed in rat tail collagen I-coated 24-well plates. Following treatment with various conditions, the cells (HPC, HPC-NC and HPC-C3a) were detached using Accutase solution (Sigma-Aldrich; Merck KGaA) and 2×10⁵ cells were allowed to attach to the collagen I-coated plates in 0.5 ml RPMI-1640 medium supplemented with 0.1% FBS. Time course adhesion assays were performed at time points 30 min, 1 h and 3 h at 37°C in a 5% CO₂ incubator. Following this, the non-adherent cells were collected, and the adherent cells were detached with Accutase solution. The numbers of both adherent and non-adherent cells were counted using a cell counter. The cell adhesion ability was represented by the ratio of adherent cells to non-adherent cells and was further calibrated to that of non-treated HPC cells (the adhesion ability of non-treated HPC cells was defined as 1.0). To compare the adhesive ability between HPC, HPC-NC, HPC-C3a and SB290157-treated HPC-C3a cells, the cells were cultured in the maturation condition for 6 days before the assay began.

RNA isolation and reverse transcription-quantitative PCR (RT-qPCR)

TRIzol^® reagent (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract total RNA from the cultured HPC, HPC-NC or HPC-C3a cells. cDNA was generated using a PrimeScriptTM RT Master Mix kit (Takara Biotechnology Co., Ltd.), according to the manufacturer's protocol, and analyzed by RT-qPCR using 1 µl of cDNA, 250 nM primers and UltraSYBR mixture (CoWin Biosciences). 18S RNA was used as the endogenous control. The sequences of the primers are presented in Table I. The following thermocycling conditions were used to detect the expression levels of nephrin: Initial denaturation at 95°C for 5 min; followed by 40 cycles of 95°C for 20 sec, 58°C for 10 sec and 72°C for 45 sec; and a final extension at 72°C for 5 min. The following thermocycling conditions were used for the rest of the genes: Initial denaturation at 95°C for 5 min; followed by 40 cycles of 95°C for 20 sec, 60°C for 20 sec and 72°C for 30 sec; and a final extension at 72°C for 5 min. The expression levels were quantified using the 2^−∆∆Cq method (23) and finally normalized to the expression levels of in the HPC control cells (which was set to 1.0).

Table I.

Primers used for reverse transcription-PCR.

Gene	Forward sequence (5′-3′)	Reverse sequence (5′-3′)
COL1A1	atcaaccggaggaatttccgt	caccaggacgaccaggttttc
COL4A1	cggatcacattgacatgaaacc	accagtccatgctcgcaaag
COL4A2	tggacctgatggaaagcgag	tgctcctttcagcccaaaca
COL4A3	ggtggcctgagagcctga	tcacagaagcactggccttt
COL4A4	ccagcccgcctccaac	gtgaggttctgtgttctgggt
FN1	gagaataagctgtaccatcgcaa	cgaccacataggaagtcccag
LAMA1	ttagccaccgggaacctaaag	gccatagcagatacacatgcct
LAMB1	tgactttcaagacattccgtcc	aggcgaagtatctatacacaccc
LAMC1	actgccactgacatcagagta	gcttgcgtgtccattacatttac
LAMA2	gccaacgctgagaaacttgag	gagctccacaaaaccaggct
LAMB2	agcaatgggcagagttggaa	ggctcaaggcagacaggatt
LAMC2	ggagctggagtttgacacgaa	agcttctgctccagtaagacc
LAMA3	gggtgctgctcaaagcaaag	gctgcagtacagaggcttca
LAMB3	cagtcacagagcaggaggtg	tctccaggtctctcggaagg
LAMC3	tacggcaaacagaacccctc	gatcagccagcagagtcctg
ITGA1	gtgcttattggttctccgttagt	cacaagccagaaatcctccat
ITGA2	tccagagtaacctccagggg	gccagggttttccagactga
ITGA3	cccagaggaccaaggaaacc	attgttcaggtctgccaggg
ITGA5	ggagaggagcctgtggagta	cctccttggcagtaaccctg
ITGB1	gtaaccaaccgtagcaaagga	tcccctgatcttaatcgcaaaac
ITGB3	gaagcagagtgtgtcacgga	acatgacactgcccgtcatt
DAG1	ggaaggaggctttgccatct	gaacacactggaggtctggg
PTK2	catgccctcaaccagggatt	cacgctgtccgaagtacagt
ILK	gcagcccgagtcccgaggata	gcgccgagtcccctggattg
NRP1	gcggacttttccagctctct	cagttggcctggtcgtcat
TLN1	agaacggaaacctgccagag	tgaattgcctggtttgcacg
TLN2	tgacctaaattggctggatcac	aacttccgtctaagcagcaac
PXN	catggacgacctcgacgc	gaggctgctggtggatgaat
FERMT2	gatgctgccctttcagacct	ctggtgtgccactggattct
VCL	cgtccgggttggaaaagaga	gacctcagcctcatcgaagg
ACTA2	gggactaagacgggaatcct	gtcacccacgtagctgtctt
WT1	cccattgccatttggtgtgg	ccgatgccttgctctctgat
TGFB1	atggagagaggactgcggat	tagtgttccccactggtccc
18S RNA	tttctcgattccgtgggtgg	agcatgccagagtctcgttc
C3aR	tgaagccttcagctactgtctcag	ggacaatgatggaggggatgag
C3a	aagtcggcaagtaccccaag	agttgcagcagtccaggaag
CPD	cccgaccagtttagcaccg	actgagccaccatgcagattt
CPM	tgtgggttcttgttgtgggg	agggtctttgccatcactgg
CPN1	ggtgattacttccggctgct	cactttggctctgactccgt
CPN2	acaccagactcctctctgct	gagcagaacacctcctggac
CPZ	gctccttacagtcctggtcg	aagtgctctgacagtgtggg
Nephrin	tggcgattcctgcctccgtt	ttctgctgggagccctcgtt

COL1A1, collagen I α1; COL4A1,collagen IV α1; COL4A2, collagen IV α2; COL4A3, collagen IV α3; COL4A4, collagen IV α4; C3aR, complement C3a receptor 1; FN1, fibronectin-1; LAMA1, laminin 1α; LAMB1, laminin 1β; LAMC1, laminin 1γ; LAMA1, laminin α1; LAMB2; laminin β2; LAMC2, laminin γ2; LAMA3,laminin α3; LAMB3, laminin β3; LAMC3, laminin γ3; ITGA1, integrin α1; ITGA2, integrin α2; ITGA3, integrin α3; ITGA5, integrin α5; ITGB1, integrin β1; ITGB3, integrin β3; DAG1, dystroglycan 1; PTK2, protein tyrosine kinase 2; ILK, integrin linked kinase; NRP1, neuropilin 1; TLN1, talin 1; TLN2, talin 2; PXN, paxillin; FERMT2, fermitin family member 2; VCL, vinculin; ACTA2, α smooth muscle actin; WT1, Wilms' tumor 1; TGFB1, transforming growth factor β1; CPD, carboxypeptidase D; CPM, carboxypeptidase M; CPN1, carboxypeptidase N1; CPN2, carboxypeptidase N2; CPZ, carboxypeptidase Z.

Western blotting

Total protein was extracted from the cultured HPC, HPC-NC or HPC-C3a cells using RIPA lysis buffer (Beyotime Institute of Biotechnology) containing a Protease Inhibitor Cocktail (Roche Diagnostics) at 4°C. Total protein was quantified using a BCA Protein Assay kit (Beyotime Institute of Biotechnology) and 10 µg protein/lane was separated via 8% SDS-PAGE, then transferred to a PVDF membrane (Merck KGaA). Following blocking with 5% skim milk powder at room temperature for 4 h, the membrane was probed with primary antibodies, including rabbit anti-human C3aR polyclonal (cat. no. sc-20138; Santa Cruz Biotechnology, Inc.; dilution 1:1,000), rabbit anti-human integrin β1 polyclonal (ITGB1; cat. no. 9699S; Cell Signaling Technology, Inc.; dilution 1:1,500), rabbit anti-human vinculin (VCL; cat. no. 26520-1-AP; ProteinTech Group, Inc.; dilution 1:1,000), rabbit anti-human protein tyrosine kinase 2 (PTK2; cat. no. 71433; Cell Signaling Technology, Inc.; dilution 1:1,000) or mouse anti-human GAPDH monoclonal (cat. no. MB001; Bioworld Technology, Inc; dilution 1:10,000) antibodies at 4°C overnight. The membranes were then incubated with horseradish peroxidase (HRP)-conjugated secondary goat anti-rabbit IgG (cat. no. HS101-01; TransGen Biotech Co., Ltd; dilution 1:2,000) or goat anti-mouse IgG (cat. no. BS12478; Bioworld Technology, Inc.; dilution 1:10,000) antibodies for 1 h at room temperature. The immunolabeled proteins were detected by chemiluminescence using the Chemiluminescent HRP substrate (Merck KGaA). Densitometric analysis was performed using ImageJ version 1.52a software (National Institutes of Health).

Cyto-immunofluorescence

The HPC, HPC-NC or HPC-C3a cells growing on glass coverslips were washed with PBS, fixed in 4% paraformaldehyde at 4°C for 30 min and permeabilized in 0.5% Triton X-100 at room temperature for 10 min. Following blocking with 3% BSA (Beyotime Institute of Biotechnology) for 30 min, the cells were incubated with rabbit anti-human C3aR (dilution 1:50) or rabbit anti-human VCL (dilution 1:100) antibodies at room temperature for 2 h. After washing with PBS three times, the cells were incubated with Cy3-labled goat anti-rabbit IgG antibodies (cat. no. 33108ES60; Shanghai Yeasen Biotechnology Co., Ltd.; dilution 1:200) at room temperature for 30 min. The cells were washed again with PBS and exposed to DAPI solution (Beyotime Institute of Biotechnology) at room temperature for 5 min. Following washing with PBS, the cells were mounted, and fluorescent images were captured with a confocal microscope (LSM-800; Zeiss GmbH; magnification, ×400). Homologous serum was used instead of the primary antibody for the control.

Measurement of C3a levels

C3a levels was measured using a human C3a ELISA kit (BD Biosciences), according to the manufacturer's protocol.

Statistical analyses

Data are presented as the mean ± SEM. SPSS software (version no. 19.0; SPSS, Inc.) was used for data analysis. Multiple comparisons were performed using one-way ANOVA and Tukey's post hoc test was used in the comparison between two groups. All statistical tests were two-tailed. P<0.05 was considered to indicate a statistically significant difference.

Results

C3aR is expressed by HPC cells

To investigate the effect of C3aR activation in HPC cells, the expression of C3aR was examined. The results of RT-qPCR, western blotting and immunofluorescence demonstrated that C3aR was expressed by HPC cells (Fig. 1). The expression of C3aR was observed on the cell surface and in the cytoplasm.

Figure 1.

Results of RT-qPCR, western blotting and immunofluorescence demonstrated that C3aR is expressed by HPC cells. Results for (A) RT-qPCR, (B) western blotting and (C) immunofluorescence staining for C3aR. Scale bar, 50 µM. Homologous serum was used instead of anti-C3aR antibody as the negative control in (C). RT-qPCR, reverse transcription-quantitative PCR; C3aR, human C3a anaphylatoxin receptor; HPC, human podocyte cell line; Md, DNA molecular weight; Neg, negative control; Mp protein molecular weight.

Secretory C3a is overexpressed in HPC-C3a cells

To determine whether C3a is overexpressed by HPC-C3a cells, C3a mRNA and protein levels were measured by RT-qPCR and ELISA, respectively. RT-qPCR results demonstrated that the expression levels of C3a mRNA was significantly increased in the HPC-C3a cells compared with HPC-NC and untransfected HPC cells (768.18±116.11 vs. 1.17±0.16 and 1.00±0.02, respectively; Fig. 2A). Furthermore, the ELISA analysis reported that the expression of C3a protein was significantly increased in the cultured medium of HPC-C3a cells compared with the HPC-NC and untransfected HPC cells (563.63±69.86 vs. 0.34±0.06 and 0.37±0.11 ng/ml, respectively; Fig. 2B).

Figure 2.

C3a was proved to be overexpressed in HPC-C3a cells compared with HPC-NC and untransfected HPC cells. The overexpression of C3a in HPC-C3a cells was confirmed at the (A) mRNA level by reverse transcription-PCR and the (B) protein level by measuring C3a in the cultured medium using an ELISA kit. ***P<0.001 vs. the HPC group; ^###P<0.001 vs. the HPC-NC group. C3a, human C3A anaphylatoxin; HPC, human podocyte cell line; NC, negative control.

Influence of overexpression of C3a in the phenotype of HPC cells during their maturation

As shown in Fig. 3A, at the proliferation condition, HPC cells grew with typical cobblestone morphology. Shifting the cells to the maturation condition resulted in the arrest of cell proliferation and differentiation. The cell bodies enlarged in a time-dependent manner and various cellular projections developed. Within two weeks, the cells converted from individual cobblestone morphology into an arborized appearance, which resembled the morphology of mature glomerular podocytes in vivo (21). Similar to the untransfected HPC cells, HPC-NC cells exhibited typical cobblestone morphology in the proliferation condition and ceased proliferation in the maturation condition. Additionally, HPC-NC cells became enlarged and developed arborized morphology within two weeks. No obvious morphological change was observed in HPC-C3a cells cultured in the proliferation condition. However, these cells failed to undergo cell body expansion, which the HPC-NC and untransfected HPC cells underwent in the maturation condition. HPC-C3a cells cultured in the maturation condition appeared to exhibit decreased adhesive capacity and became extremely sensitive to the regular change of the medium. Increased contracted cells, which could be easily detached from the surface of the culture plate with gentle shaking, were observed in the HPC-C3a group starting on the 5th day following transference to the maturation condition and the regular change of medium would induce contraction of the cells immediately (within <30 min). The cell numbers of HPC-C3a decreased markedly since about the 6th day. Within 2 weeks, most of the HPC-C3a cells were lost and the cells still remained in culture plate failed to develop into the arborized appearance as the untreated HPC and HPC-NC cells did. Addition of SB290157 (SB) blocked the phenotypic alterations caused by Lenti-C3a infection. The morphological differences in the formation of the arborized morphology, which was observed in HPC-NC and SB290157-treated HPC-C3a cells, but not in HPC-C3a cells, were more clearly pronounced under the fluorescence microscope since HPC-NC and HPC-C3a cells have fluorescence due to the expression of EGFP (Fig. 3B). However, as the untransfected HPC cells have no fluorescence, these cells were not observed under the fluorescence microscope. Furthermore, HPC-C3a cells exhibited decreased adhesion capacities compared with the C3a group, as confirmed by the adhesion assays (Fig. 3C).

Figure 3.

Influence of C3A anaphylatoxin overexpression on the morphology and adhesion ability of HPC cells during maturation. (A) Representative images taken under a phase contrast microscope demonstrating the morphology of HPC, NC, C3a cells and C3a cells treated with 1 µM of SB290157 cultured during the maturation condition for 0, 2, 4, 7 and 14 days. Scale bar, 100 µM. (B) Representative images taken with a fluorescence microscope revealing the morphology of NC, C3a and SB cells cultured during the maturation condition for 0, 2, 4, 7 and 14 days. As normal HPC cells do not exhibit fluorescence, the untransfected HPC cells were not included in the cell morphology observational experiments under fluorescence microscopy. Scale bar, 50 µM. (C) Results of adhesion analysis. The adhesion ability of HPC, NC, C3a and SB cells was analyzed following culturing in the maturation condition for 6 days. **P<0.01 vs. the HPC group; ^##P<0.01 vs. the NC group; ^&&P<0.01 vs. the C3a group. C3a, HPC cells overexpressing human C3A anaphylatoxin; HPC, human podocyte cell; NC, HPC cells transfected with negative control vector; SB, C3a cells treated with SB290157.

Effect of C3a overexpression on the structure of focal adhesion (FA)

FAs are macromolecular complexes located at the sites of cell adhesion to extracellular matrices (ECMs) and serve a key role in cell adhesion to the ECM (24). To determine whether the decreased adhesion capacity of HPC-C3a cells is associated with the alteration of FAs, the cells were stained with antibodies against VCL, a core component of FAs. Different immunostaining patterns for VCL were observed in the HPC-C3a cells compared with the untransfected HPC and HPC-NC cells (Fig. 4). Immunostaining was observed mainly in the center of HPC-C3a cells and fewer and smaller VCL-positive plaques were observed in the peripheral area of the cells. SB290157 treatment (SB) blocked the VCL-induced alterations in the HPC-C3a cells.

Figure 4.

Overexpression of C3a markedly alters the immunostaining pattern of VCL. All cells (HPC, NC, C3a and SB) were grown on glass coverslips. Following culturing in the maturation condition for 6 days, the cells were fixed and stained for VCL. Homologous serum was used instead of anti-VCL antibody as the negative control (negative immuno-staining group). Scale bar, 50 µM. C3a, human C3A anaphylatoxin; VCL, vinculin; HPC, human podocyte cell line; NC, negative control; SB, SB290157.

Effect of C3a overexpression on the expression of cell adhesion-associated genes

To explore the underlying mechanism of decreased adhesion capacity in HPC-C3a cells, the expression changes of ECM cell adhesion-associated genes were observed. A total of 33 genes that have been reported to be associated with podocyte adhesion were examined. These included 15 genes [collagen I α1 (COL1A1), collagen IV α1 (COL4A1), collagen IV α2 (COL4A2), collagen IV α3 (COL4A3), collagen IV α4 (COL4A4), fibronectin-1 (FN1), laminin β1 (LAMB1), laminin α1 (LAMA1), laminin γ1 (LAMC1), laminin β1 (LAMB1), laminin β2 (LAMB2), laminin γ2 (LAMC2), laminin α3 (LAMA3), laminin β3 (LAMB3) and laminin γ3 (LAMC3)] encoding ECM components, 6 genes [integrin α1 (ITGA1), integrin α2 (ITGA2), integrin α3 (ITGA3), integrin α5 (ITGA5), ITGB1 and integrin β3 (ITGB3)] encoding integrins and 9 genes [dystroglycan 1 (DAG1), PTK2, integrin linked kinase (ILK), neuropilin 1 (NRP1), talin 1 (TLN1), talin 2 (TLN2), paxillin (PXN), fermitin family member 2 (FERMT2) and VCL] encoding cytoplasmic molecules associated directly with FAs. Additionally, the gene encoding Wilms' tumor 1 (WT1), the molecular marker for podocytes, α smooth muscle actin (ACTA2), the molecular marker for mesenchymal cells and for transforming growth factor β1 (TGFB1), an important molecule in regulation of cell proliferation, differentiation, growth, survival, apoptosis, adhesion and migration, were examined. The RT-qPCR results revealed that the expression levels of COL1A1, FN1, LAMA1, LAMB1, LAMC1, ITGA1, ITGA2, ITGB1, PTK2, NRP1, TLN2, FERMT2 and VCL mRNA in HPC-C3a cells were significantly decreased compared with these levels in the untransfected HPC and HPC-NC cells. However, the addition of SB290157 prevented or reduced the decrease in the expression of COL1A1, FN1, LAMA1, LAMB1, LAMC1, ITGA1, ITGA2, ITGB1, PTK2, NRP1, TLN2, FERMT2 and VCL gene (Table II). No significant changes in the expression of COL4A1, COL4A2, COL4A3, COL4A4, LAMA2, LAMB2, LAMC2, LAMA3, LAMB3, LAMC3, ITGA3, ITGA5, ITGB3, DAG1, ILK, PXN, TLN1, PXN, ACTA2, WT1 and TGFB1 were observed between groups.

Table II.

Expression levels of cell adhesion-associated genes.

	Relative mRNA level
Gene	HPC	HPC-NC	HPC-C3a	HPC-C3a+SB
COL1A1	1±0.06	1.19±0.34	0.28±0.04b,c	0.75±0.11d
COL4A1	1±0.05	1.08±0.31	1.17±0.17	1.14±0.16
COL4A2	1±0.06	0.95±0.09	1.04±0.11	1.00±0.12
COL4A3	1±0.10	1.36±0.39	1.34±0.43	1.15±0.15
COL4A4	1±0.06	1.08±0.07	1.02±0.22	0.97±0.04
FN1	1±0.13	1.02±0.28	0.19±0.05b,c	0.62±0.09a,d
LAMA1	1±0.16	0.98±0.16	0.19±0.03b,c	0.80±0.12e
LAMB1	1±0.07	1.01±0.08	0.21±0.07b	0.79±0.04b,e
LAMC1	1±0.16	1.07±0.19	0.18±0.03b,c	0.75±0.04a,e
LAMA2	1±0.24	0.99±0.12	1.32±0.40	0.94±0.19
LAMB2	1±0.03	0.97±0.13	1.00±0.17	0.96±0.22
LAMC2	1±0.12	0.93±0.16	1.00±0.18	0.92±0.05
LAMA3	1±0.05	1.05±0.20	1.12±0.15	0.92±0.05
LAMB3	1±0.07	1.04±0.19	1.11±0.25	1.00±0.02
LAMC3	1±0.05	0.94±0.33	1.22±0.29	1.05±0.12
ITGA1	1±0.22	0.94±0.15	0.40±0.04b,c	0.92±0.03e
ITGA2	1±0.14	0.86±0.29	0.26±0.08b,c	0.90±0.14e
ITGA3	1±0.18	0.92±0.09	0.98±0.09	0.92±0.13
ITGA5	1±0.13	1.07±0.24	0.98±0.40	1.06±0.22
ITGB1	1±0.14	0.86±0.29	0.27±0.07b,c	0.80±0.15e
ITGB3	1±0.18	1.05±0.18	1.11±0.15	1.09±0.10
DAG1	1±0.15	1.05±0.11	1.08±0.10	1.04±0.16
PTK2	1±0.26	0.85±0.10	0.38±0.08b,c	0.80±0.09e
ILK	1±0.04	0.98±0.13	1.01±0.11	0.96±0.07
NRP1	1±0.08	1.06±0.14	0.45±0.12b,c	0.90±0.19e
TLN1	1±0.08	0.93±0.11	0.98±0.16	0.92±0.15
TLN2	1±0.12	0.92±0.15	0.48±0.08b,c	0.86±0.08e
PXN	1±0.14	1.14±0.25	1.24±0.05	0.93±0.21
FERMT2	1±0.15	0.94±0.11	0.55±0.15b,c	0.90±0.09d
VCL	1±0.11	1.00±0.13	0.60±0.02b,c	090±0.19d
ACTA2	1±0.10	0.96±0.19	0.99±0.12	0.93±0.18
WT1	1±0.11	0.90±0.07	0.97±0.13	0.91±0.15
TGFB1	1±0.11	0.94±0.22	0.90±0.13	0.97±0.19

^aP<0.05

^bP<0.01 vs. the HPC group.

^cP<0.01 vs HPC-NC group.

^dP<0.05

^eP<0.01 vs. the HPC-C3a group. Values are presented as mean ± SEM. HPC, human podocyte cell; NC, negative control; C3a, C3a, human C3A anaphylatoxin; SB, SB290157. COL1A1, collagen I α1; COL4A1,collagen IV α1; COL4A2, collagen IV α2; COL4A3, collagen IV α3; COL4A4, collagen IV α4; C3aR, complement C3a receptor 1; FN1, fibronectin-1; LAMA1, laminin 1α; LAMB1, laminin 1β; LAMC1, laminin 1γ; LAMA1, laminin α1; LAMB2; laminin β2; LAMC2, laminin 2γ; LAMA3,laminin α3; LAMB3, laminin β3; LAMC3, laminin 3γ; ITGA1, integrin α1; ITGA2, integrin α2; ITGA3, integrin α3; ITGA5, integrin α5; ITGB1, integrin β1; ITGB3, integrin β3; DAG1, dystroglycan 1; PTK2, protein tyrosine kinase 2; ILK, integrin linked kinase; NRP1, neuropilin 1; TLN1, talin 1; TLN2, talin 2; PXN, paxillin; FERMT2, fermitin family member 2; VCL, vinculin; ACTA2, α smooth muscle actin; WT1, Wilms' tumor 1; TGFB1, transforming growth factor β1; CPD, carboxypeptidase D; CPM, carboxypeptidase M; CPN1, carboxypeptidase N1; CPN2, carboxypeptidase N2; CPZ, carboxypeptidase Z.

Additionally, the decreased expression of ITGB1, PTK2 and VCL in HPC-C3a cells was confirmed by western blotting compared with HPC and HPC-NC cells; this decrease was blocked by addition of 1 µM SB290157 (Fig. 5).

Figure 5.

Overexpression of C3a significantly decreased the levels of VCL, PTK2 and ITGB1 in HPC cells. All cells (HPC, NC, C3a and SB) were cultured in the maturation condition for 6 days. Total proteins were extracted and (A-C) western blotting and (D-F) statistical analysis for VCL, PTK2 and ITGB1 was performed. *P<0.05, **P<0.01 vs. the HPC group; ^#P<0.05, ^##P<0.01 vs. HPC-NC group; ^&P<0.05, ^&&P<0.01 vs. the C3a group. C3a, human C3A anaphylatoxin; PTK2, protein tyrosine kinase 2; ITGB1, integrin β1; HPC, human podocyte cell line; NC, negative control; SB, SB290157; VCL, vinculin.

Given the importance of nephrin in the maintenance of the structure and function of podocytes, the expression of the nephrin gene was examined by RT-qPCR. However, no significant differences were observed between groups (data not shown).

Addition of C3a to the medium only partially mimicked the effect of overexpression of C3a in HPC cells

To further examine the effect of C3aR activation on the maturation of HPC cells, the cells were treated with purified human C3a. Similar to the effect of C3a overexpression, the expression of FN1, LAMB1, FERMT2, LAMA1 and LAMC1 in HPC cells treated with C3a for 6 days (adding purified C3a every two days) was significantly decreased compared with the untreated HPC group. The addition of SB290157 prevented the decrease in the expression of FN1, LAMB1, FERMT2, LAMA1 and LAMC1 in HPC cells. However, C3a had no significant effect on the expression of COL1A1, ITGA1, ITGA2, ITGB1, PTK2, NRP1, TLN2 or VCL. Additionally, C3a failed to induce obvious change in the distribution of VCL-positive FAs, adhesive capacity or the detachment of cells (Fig. 6).

Figure 6.

Addition of C3a to the medium only partially mimics the effect of overexpression of C3a in HPC cells. HPC cells were divided into three groups: HPC, HPC+SB+C3a and HPC+C3a. Morphological changes were observed under a phase contrast microscope. (A) Reverse transcription-PCR analysis of the expression of focal adhesion-associated genes. *P<0.05, **P<0.01 vs HPC group; ^#P<0.05, ^##P<0.01 vs HPC+C3a group. (B) Immunofluorescence staining for VCL. Scale bar, 50 µM. (C) Representative images taken under phase contrast microscopy demonstrated cell morphology. Scale bar, 100 µM. (D) Results of adhesion analysis. COL1A1, collagen I α1; FN1, fibronectin 1; LAMA1, laminin α1; LAMB1, laminin β1; LAMC1, laminin γ1; ITGA1, integrin α1; ITGA2, integrin α2; ITGB1, integrin β1; PTK2, protein tyrosine kinase 2; NRP1, neuropilin 1; TLN2, talin 2; FERMT2, fermitin family member 2; VCL, vinculin; C3a, human C3a anaphylatoxin; HPC, human podocyte cell; SB, SB290157.

HPC cells express carboxypeptidases

Given that C3a is easily inactivated by carboxypeptidases by cleaving the C-terminal arginine in vivo (25–27), we hypothesized that inactivation of C3a may be one of the causes for the different effects observed between C3a overexpression and addition of purified C3a. To investigate this hypothesis, the expression of carboxypeptidases in HPC cells was examined. As presented in Fig. 7, the results of RT-PCR demonstrated that although carboxypeptidases N1 (CPN1) and N2 (CPN2) were not expressed in HPC cells, carboxypeptidases Z (CPZ), M (CPM) and D (CPD) were indeed expressed in the cells, supporting our hypothesis that the C3a added to the HPC cultured medium may be degraded by the carboxypeptidases produced by the cells.

Figure 7.

Results of reverse transcription-PCR demonstrated that human podocyte cells express genes for carboxypeptidases Z, M and D. However, cells did not express genes for carboxypeptidases N1 or N2. Md, DNA molecular marker; D, carboxypeptidase D; M, carboxypeptidase M; Z, carboxypeptidase Z; N1, carboxypeptidase N1; N2, carboxypeptidase N2; nD, nM, nZ, nN1 and nN2, negative controls for carboxypeptidases D, M, Z, N1 and N2, respectively.

Discussion

To the best of our knowledge, the present study is the first to investigate the effect of the C3a anaphylatoxin receptor (C3aR) activation on the maturation of podocytes by using human podocyte (HPC) cells. Similar to a previous report (21), HPC cells proliferated and exhibited typical cobblestone morphology when cultured in the proliferation condition. Proliferation ceased and the cells gradually differentiated into mature podocytes with enlarged cell bodies and arborized appearance in the maturation condition. Infection of HPC cells with control lentivirus did not induce an obvious change in the cells. However, infection of HPC cells with Lenti-C3a, which increased the levels of secretory C3a in the cells, induced marked phenotypic changes, including significantly decreased adhesion capacity, increased tendency to contract and to be detached from the surface of tissue culture plates and failure to expand cell bodies and develop arborized appearance.

Proper attachment of cells to the extracellular matrix (ECM) is a basic requirement to build a multicellular organism. It is the basis for adherent cells to survive and function normally. Given the key role of cell adhesion to the ECM in the behavior of cells, including cellular differentiation, expansion, survival, apoptosis and the maintenance of cell morphology (24,28), it was hypothesized that the markedly decreased adhesion capacity of the cells may be the fundamental cause for the different phenotypes observed in the HPC-C3a cells. In accordance with this hypothesis, addition of the C3aR-specific antagonist SB290157 prevented the decrease in cell adhesion capacity, along with the recovery of the ability for the cells to expand their cell bodies and develop into the arborized appearance.

Cells, including podocytes, attach to ECM mainly through the formation of FAs. FAs are large macromolecular assemblies consisting of ECM components, ECM receptors (integrins) and certain cytoplasmic proteins, and serve as the linkages between the ECM and cellular skeleton (29,30). In addition to anchoring cells, FAs function as signal sensors, which inform the cells about the condition of the ECM to influence the cells to alter their behaviors in response to survival, apoptosis, spreading, differentiation and migration (24). To determine whether the decreased adhesion capacity observed in the HPC-C3a cells was associated with the impairment of FAs, the changes in the structure of FAs was examined by immunostaining for VCL. As a core component of FAs, VCL comprises an N-terminal head domain, a proline-rich linker and a C-terminal tail domain. By binding to talin by its head domain (31) and to paxillin, F-actin and phosphatidylinositol- 4,5-bisphosphate by its tail domain (32,33), VCL serves a pivotal role in stabilizing FAs and controls the potency of cell adhesion binding to ECM (34,35). As expected, C3a overexpression markedly altered the distribution of VCL in HPC cells. Additionally, C3aR inhibition with SB290157 blocked this alteration.

To further explore the cause of the decreased adhesion ability in the HPC-C3a cells, the changes in the expression of FA structure- and function-associated genes was examined. Overexpression of C3a significantly reduced the expression level of FAs-associated genes, including the genes encoding for LAMA1, LAMB1, LAMC1, FN1, COL1A1, ITGA1, ITGB1, ITGA2, VCL, TLN2, PTK2, NRP1 and FERMT2. LAMA1, LAMB1 and LAMC1 are the three chains of laminin 1. Laminin, collagen and fibronectin are well-known components of the ECM. By interacting with their receptors, ECM components provide mechanical support for cells and have an important role in regulating cell shape and function. For instance, laminin 1 has been reported to serve an important role in orchestrating the development and differentiation of neural cells including extension, migration, survival and neurite outgrowth (36–38). Additionally, similar roles have been demonstrated in other ECM components (39). Laminin 1, collagen 1 and fibronectin 1 exert their cellular regulatory roles by interacting with integrins, including α1β1, α2β1 and α3β1. By binding to their ECM ligands by their extracellular ‘lobster claws’ domain and interacting with the cytoskeleton system via their cytoplasmic parts, integrins bridge the ECM and cytoskeleton, and are therefore the structural center of the FAs (40,41). Additionally, integrins serve a key role in signal transduction associated with cell growth, division, survival, differentiation and apoptosis (40). Integrins are obligate heterodimers and each integrin has two subunits: α and β. Mammals exhibit 24 α and 9 β integrins. Among them, integrins α1, α2 and β1 have been reported to be expressed by podocytes and α1β1, α2β1 and α3β1 have been well documented to serve important role in the normal attachment of podocytes to the glomerular basement membrane (42). Genetic deficit or decreased expression of these integrins have been demonstrated to be implicated in abnormal renal development (43), decreased adhesion capacity of glomerular podocytes, podocyte damage (44,45) and renal dysfunction (46,47). VCL, TLN2, PTK2, NRP1 and FERMT2 are the major cytoplasmic components associated with FAs. Either by connecting integrins to cytoskeleton or by mediating signal transduction, these proteins exert their roles in cell adhesion and adhesion-associated cellular function (48–51). Thus, the significantly decreased expression of FA-associated genes explained the decreased adhesion ability of HPC-C3a cells observed in the current study.

As an important component of the slit diaphragm, nephrin serves a key role in podocyte-associated nephropathy. However, the current study did not observe significant change in the expression of nephrin by C3a overexpression in an RT-qPCR assay.

The present study observed that the effect of C3a overexpression on the phenotype of HPC cells was not completely mimicked by direct addition of C3a to the cell culture medium. In vivo, C3a is easily inactivated by carboxypeptidases, which catalyze the removal of the C-terminal arginine of C3a (25). There are various carboxypeptidases. Among them, CPN is produced mainly by the liver, circulates in the plasma and contributes to the inactivation of anaphylatoxins including C3a, C4a and C5a (26). Furthermore, CPM, CPD and CPZ have been reported to be able to remove the C-terminal arginine of peptides (27). Moreover, they were hypothesized to function outside the cell and function normally at neutral pH (27). To investigate the cause for the different effects between the overexpression of C3a and the direct addition of C3a on HPC cells, the expression of CPN (encoded by the genes CPN1 and CPN2), CPM, CPD and CPZ genes in HPC cells were examined. HPC cells expressed CPM, CPD and CPZ genes; however, the cells did not express CPN. Therefore, it seems reasonable to hypothesize that directly added C3a may be inactivated by HPC cell derived carboxypeptidases, including CPM, CPD and CPZ, and thus can only activate C3aR in HPC cells temporarily. This is quite different compared with C3a overexpression in the cells, in which C3a is produced continuously and, therefore, may induce sustained receptor activation.

The present studies had certain limitations. Firstly, as the current study was an in vitro study, in vivo investigations are required. Secondly, while the present study only observed the influence of C3a overexpression in the morphological maturation of podocytes, the pathways through which the overexpressed C3a may exert its role remain to be clarified. Lastly, as all the commercial kits detected both C3a and C3a desArg (the inactivated form of C3a), it could not be confirmed whether the added C3a was directly inactivated by podocyte-derived carboxypeptidases.

In summary, through the overexpression of the secretory C3a in HPC cells, the current study investigated the effect of sustained activation of C3aR on the maturation of HPC cells. The results demonstrated that sustained activation of C3aR impaired the morphological maturation of HPC cells. This effect may be associated with the decreased adhesion ability of the cells to the ECM and caused by the decreased expression of FA-associated genes. These results revealed novel function of C3aR signaling in podocytes and provided novel insight into the mechanism underlying the regulation of podocyte morphology development. Presently, it is not clear whether the overactivation of C3aR induces impairment of podocyte maturation in vivo. However, chronically increased complement activation has been reported in renal diseases (52–56), which may indicate increased C3a and the chronic activation of C3aR in renal cells, including podocytes and renal progenitor cells [for instance, parietal epithelial cells (57–61)]. The possible pathological role of excessive C3aR signaling in the dysregulation of podocyte architecture and podocyte regeneration requires further research.

Funding

The current study was supported by the Science Foundation of Taizhou City (grant no. 1801ky02).

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

JMZ designed the current research, performed experiments and drafted and revised the manuscript. SSW and XT performed the experiments, analyzed data and drafted and revised the manuscript. DJC participated in data analysis and revised the manuscript. All authors read and approved the final manuscript and agree to be accountable for all aspects of the research in ensuring that the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Ethics approval and consent to participate

Not applicable.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.