Table 1.
Granulocytic Targets Used for Mass Cytometry, Clones of Antibodies, Functional Description, and Population Distributions
| Target | Clone | Description/Function | Distribution | References |
|---|---|---|---|---|
| CD203c (E-NPP3) | NP4D6 | Transmembrane ectoenzyme; clearance of extracellular nucleotides | B | (Hennersdorf et al., 2005), (Buhring et al., 2001) |
| CD13 | WM15 | Transmembrane zinc metallopeptidase; cytokine processing | B N | (Hennersdorf et al., 2005), (Terstappen et al., 1990a), (Stroncek et al., 1998) |
| CD193 (CCR3) | 5 × 108 | G-protein coupled transmembrane receptor; chemokine receptor | B E | (Uguccioni et al., 1997), (Daugherty et al., 1996) |
| CD14 | M5E2 | Transmembrane glycoprotein; LPS receptor | B N E | (Iida et al., 1994), (Terstappen et al., 1990a) |
| CD244 (2B4) | C1.7 | CD2 family transmembrane receptor; inhibition and activation of NK cells | B E | (Hennersdorf et al., 2005), (Munitz et al., 2005) |
| FcεRIa | CRA-1 | Ig superfamily, transmembrane receptor; high affinity IgE receptor | B | (Valent, 1994) |
| CD123 | 6H6 | Transmembrane glycoprotein; IL3 receptor | B | (Hennersdorf et al., 2005), (Agis et al., 1996) |
| CD44 | IM7 | Adhesion molecule; immune memory and activation | B N E | (Valent et al., 1990), (Mikecz et al., 1995) |
| MRP-14 (calgranulin B) | MRP 1H9 | S100 family of protein; calcium-dependent activation | N | (Hessian et al., 1993) |
| CD15 | W6D3 | Poly-N-acetyllactosamine; adhesion molecule | N E | (Skubitz and Snook, 1987), (Terstappen et al., 1990b) |
| CD16 | 3G8 | Ig superfamily, transmembrane receptor; low-affinity IgG receptor | B N | (Terstappen et al., 1990b), (Huizinga et al., 1988), (Meknache et al., 2009) |
| CD11b | M1/70 | Transmembrane glycoprotein; adhesion molecule, chemotaxis, neutrophil activation | N | (Mann and Chung, 2006), (Terstappen et al., 1990b), (de Haas et al., 1994) |
| CD66b | 80H3 | Transmembrane glycoprotein; adhesion molecule | N | (de Haas et al., 1994), (Skubitz et al., 1995) |
| CD116 (GMCSF-r) | 4H1 | Transmembrane glycoprotein; cell proliferation, differentiation | B N E | (Valent, 1994), (DiPersio et al., 1988) |
| CD33 | WM53 | Transmembrane glycoprotein; adhesion molecule | B N E | (Terstappen et al., 1990a) |
| CD305 (LAIR-1) | NKTA255 | Transmembrane protein; inhibition of cell cytotoxicity, cell activation, proliferation, and differentiation | B E | (Verbrugge et al., 2006), (Meyaard, 2008) |
| CD53 | HI29 | Transmembrane tetraspan family receptor; signal transduction, B cell activation | N E | (Matsumoto et al., 1999), (Mollinedo et al., 1998) |
| CD88 (C5aR) | S5/1 | C5a receptor; chemotaxis, granule enzyme release, and superoxide anion production | N E | (Hugli and Muller-Eberhard, 1978), (Gerard et al., 1989) |
| CD183 | CXCR3-173 | G protein-coupled transmembrane receptor; CXC-chemokine receptor | E | (Jinquan et al., 2000) |
| CD191 (CCR1) | TG4/CCR1 | G-protein coupled transmembrane receptor; cell migration | B E | (Florian et al., 2006; Ponath et al., 1996) |
| CD294 (CRTH2) | BM16 | Seven-transmembrane protein coupled with G proteins, chemotaxis | B E | (Hirai et al., 2001) |
| CD52 | HI186 | Transmembrane glycoprotein | E | (Elsner et al., 1996) |
| CD49d (VLA-4) | 9F10 | Transmembrane glycoprotein; cell trafficking and inflammation | E | (Dobrina et al., 1991), (Sriramarao et al., 1994) |
| CD7 | CD7-6B7 | Ig superfamily transmembrane glycoprotein; adhesion molecule | NK T | (Rabinowich et al., 1994) |
Our antibody panel is composed of lineage markers and proteins that have been reportedly expressed under homeostasis or generally associated with granulocyte activation. B = basophils, E = eosinophils, N = neutrophils, NK = NK cells, and T = T cells. See also Table S1.