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. 2020 Oct 26;9:e60234. doi: 10.7554/eLife.60234

Figure 8. Loss of Gli2 disrupts midline but not lateral cell shape.

(A,D) Lateral (A) and midline (D) cells in Gli2 mutant embryos and wild-type littermate controls (WT). Cells are labeled with N-cadherin or phalloidin (top) and color coded by apical area (bottom). (B,C,E,F) Average apical cell area (B,E) and apical area distributions (C,F) of lateral and midline cells from WT and Gli2 mutant embryos. (G) XZ reconstructions of lateral and midline cells labeled with F-actin in WT and Gli2 mutant embryos. (H) Cell height in lateral and midline cells in WT and Gli2 mutant embryos. A single value was obtained for each embryo and the mean ± SD is shown. n = 5 embryos/genotype, **p<0.01, ***p<0.001, Welch’s t-test (B,E) or Brown-Forsythe one-way ANOVA test (H). See Supplementary file 1 for n and p values. Embryos are 7–9 somites. Anterior up in (A,D), apical up in (G). Bars, 20 μm.

Figure 8.

Figure 8—figure supplement 1. Gli2 is required for FoxA2 expression in the ventral neural plate.

Figure 8—figure supplement 1.

(A) Single z-planes of Gli2 mutant and wild-type littermate control (WT) embryos at the level of the floor plate of the midbrain neural plate (top) or at the level of the underlying notochord (bottom) labeled with the ventral/floor plate marker FoxA2 and counterstained with phalloidin (F-actin). Note that FoxA2 is expressed in the notochord but not the floor plate of Gli2 mutants. (B) Maximum-intensity projections of WT control and Gli2 mutant neural plates labeled with the ventral marker Nkx6.1 and phalloidin. Embryos are 7–8 somites. Anterior up. Bars, 100 μm.
Figure 8—figure supplement 2. Analysis of mediolateral cell orientation in Gli2 mutants.

Figure 8—figure supplement 2.

(A,C) Percentage of lateral cells (A) and midline cells (C) with a mediolateral (ML) orientation (0–45° relative to the ML axis) or an anterior-posterior (AP) orientation (45–90° relative to the ML axis) in Gli2 mutants and wild-type littermate controls (WT). (B,D) Average ratio of cell length along the ML and AP axes. n = 5 embryos/genotype, two-way ANOVA test (A,C) or Welch’s t-test (B,D). See Supplementary file 1 for n and p values.