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. Author manuscript; available in PMC: 2020 Nov 11.
Published in final edited form as: Neuroreport. 2008 Apr 16;19(6):687–690. doi: 10.1097/WNR.0b013e3282fbcef7

Fig. 4.

Fig. 4

Rat brain chromatin immunoprecipitation (ChIP) experiments. (a) Fetal rat brain ChIP-derived DNA was used as template for PCR reactions designed to amplify the rat β4 promoter. Histone H4 (lane 2) and input (lane 9) positive controls produced the expected product. Amplification was also observed for Sp1, Sp3, Sox10 and c-Jun PCR reactions (lanes 4–7). ChIP negative controls, normal mouse IgG (lane 3), mock immunoprecipitation (lane 8) and no-template PCR control (lane 10) produced no amplified products. The arrow indicates the 200-base pair marker (lane 1). (b) Adult rat brain ChIP-derived DNA was used in PCR reactions designed to amplify the rat β4 promoter. Again, both the histone H4 (lane 2) and input (lane 9) positive controls produced the expected product. Amplification was also observed for Sp1, Sp3, Sox10 and c-Jun PCR reactions (lanes 4–7). Finally, ChIP negative controls, normal mouse IgG (lane 3), mock immunoprecipitation (lane 8) and no-template PCR control (lane 10) produced no amplified products. The arrow indicates the 200-base pair marker (lane 1). Each ChIP experiment was carried out a minimum of three times.