. 2020 Oct 22;25(21):4894. doi: 10.3390/molecules25214894

Table 3.

Comparison of classification capacities obtained for individual media and 3 different matrices using main spectra (MSPs) dendrogram analysis (via MALDI Biotyper 3.0) as well as hierarchical cluster analysis (HCA) and principal components analysis (PCA) via R software expressed as a percentage share of correctly classified strains among 7 investigated S. aureus strains. An increase in the color intensity in the column means a higher value for the parameter.

Classification Capacities Values [%] Calculated for all Investigated Strains
	MSPs Dendrograms Analysis				HCA Analysis				PCA Analysis
	Matrix				Matrix				Matrix
Medium	HCCA	DHB	SDHB	Mean	HCCA	DHB	SDHB	Mean	HCCA	DHB	SDHB	Mean
MHA	43	29	14	29	43	43	43	43	14	29	0	14
TSA	43	29	14	29	100	86	57	81	43	0	0	14
COL	29	71	43	48	57	43	57	52	29	29	29	29
BHI	71	57	29	52	71	57	43	57	29	0	0	10
BLA	71	57	43	57	100	43	86	76	71	14	29	38
CHRA	29	29	57	38	100	43	14	52	29	14	14	19
VRE	71	29	57	52	100	57	43	67	29	57	43	43
MAN	57	57	57	57	100	71	86	86	71	43	29	48
Mean	52	45	39		84	55	54		39	23	18
Classification Capacities Values [%] Calculated for Resistant Strains
	MSPs Dendrograms Analysis				HCA Analysis				PCA Analysis
	Matrix				Matrix				Matrix
Medium	HCCA	DHB	SDHB	Mean	HCCA	DHB	SDHB	Mean	HCCA	DHB	SDHB	Mean
MHA	66	33	33	44	33	66	33	44	33	33	0	22
TSA	33	66	0	33	100	66	66	77	33	0	0	11
COL	33	66	66	55	66	66	66	66	33	33	66	44
BHI	100	66	66	77	66	66	66	66	0	0	0	0
BLA	66	66	66	66	100	33	100	78	100	0	0	33
CHRA	66	66	33	55	100	66	0	55	33	33	0	22
VRE	66	33	66	55	100	66	66	77	33	100	0	44
MAN	66	66	66	66	100	100	100	100	100	66	33	66
Mean	62	58	50		83	66	62		46	33	12

MSP-main spectrum (peak lists generated from raw spectra by algorithms included in MALDI Biotyper), TSA-Tryptic Soy Agar, MHA-Mueller Hinton Agar, COL-Columbia Agar Base, BHI-Brain Heart Infusion Agar, BLA-Columbia Blood Agar, CHRA-CHROMagarTM Orientation, MAN-Mannitol Salt Agar, VRE-Vancomycin Resistant Enterococci Agar Base, HCCA-α-Cyano-4-hydroxycinnamic acid, DHB-2,5-dihydroxybenzoic acid, SDHB-mixture of DHB and 2-hydroxy-5-methoxybenzoic acid (9:1 w/w).