Abstract
Background
The aim of this study was to identify associations between dietary intakes of eggs and cholesterol and all‐cause and heart disease mortality in a US population.
Methods and Results
Data from the National Health and Nutrition Examination Survey 1999–2014 were used in this study, which included 37 121 participants ≥20 years of age. Dietary information was assessed via 24‐hour dietary recalls at baseline. Mortality status was documented until December 31, 2015. Cox proportional hazards models were used to examine the associations between dietary intakes of eggs and cholesterol and all‐cause and heart disease mortality. During a median follow‐up of 7.8 years, 4991 deaths were documented, including 870 deaths from heart disease. No significant association was observed between additional daily consumption of half an egg and all‐cause mortality (multivariable‐adjusted hazard ratio, 1.04; 95% CI, 0.96–1.13), or heart disease mortality (0.96; 0.80–1.14). Each 50‐mg/day increase of cholesterol intake was inversely associated with all‐cause mortality among participants with daily intake <250 mg (0.87; 0.77–0.98), but positively associated with all‐cause mortality among participants with daily intake ≥250 mg (1.07; 1.01–1.12). No significant association was found between dietary cholesterol intake and heart disease mortality.
Conclusions
No significant association was found between egg consumption and mortality in US adults. The association between dietary cholesterol intake and all‐cause mortality depended on the baseline intake levels, with an inverse association in those with lower intake levels (<250 mg/day) but a positive association in those with higher intake levels (≥250 mg/day).
Keywords: cardiovascular disease, cohort study, dietary cholesterol, egg, mortality
Subject Categories: Diet and Nutrition, Epidemiology
Nonstandard Abbreviations and Acronyms
- BMI
body mass index
- CVD
cardiovascular disease
- HDL
high‐density lipoprotein
- LDL
low‐density lipoprotein
- NHANES
National Health and Nutrition Examination Survey
Clinical Perspective
What Is New?
In this cohort of representative adults in the United States, we did not find a significant association between dietary intake of eggs and all‐cause or heart disease mortality.
Dietary cholesterol intake was not significantly associated with heart disease mortality, but showed a U‐shaped association with all‐cause mortality: inverse association was found among participants with intake levels <250 mg/day and a positive association was found among individuals with intake levels ≥250 mg/day.
What Are the Clinical Implications?
Our results indicate that eggs can be a component in a healthy diet as recommended in the recent 2015–2020 Dietary Guidelines for Americans, but there remain concern with high dietary cholesterol intake.
Eggs are a major dietary source of cholesterol in daily life. In addition to cholesterol, they contain high‐quality protein and several vitamins and minerals.1 Although they are affordable and nutrient‐dense, there are concerns over potential cardiovascular comlications associated with high dietary intake of eggs because of the high level of dietary cholesterol in eggs that may lead to worsening of blood cholesterol levels. For decades, it has been advised to restrict consumption of eggs, particularly yolk, but further evidence indicated that eggs were not significantly associated with adverse cardiovascular outcomes.2, 3, 4
The 2015–2020 Dietary Guidelines for Americans recommended egg consumption as part of a healthy diet, and abolished the limit of dietary cholesterol intake of 300 mg/day2 that has been in the American dietary guidelines since 1968.5 The updated recommendation is based on evidence from some major meta‐analyses of observational studies that reported no significant associations between egg consumption, up to 1 per day, and risk of cardiovascular disease (CVD) or coronary heart disease.6, 7, 8 A possible protective association with stroke was even reported.9 Accordingly, the latest science advisory from the American Heart Association supported daily consumption of 1 whole egg in healthy individuals with normal cholesterol level.10 In contrast, in early 2019, a pooled analysis of 6 US prospective cohorts reported positive associations between dietary intakes of egg and cholesterol and risk of incident CVD and all‐cause mortality.11 The reasons for the discrepancy between this study and earlier meta‐analyses are unclear, although it is possible that higher risk may only become apparent at higher doses: the average dietary cholesterol intake was 293 mg/day in US adults in 2013–2014,12 much higher than the global average of 228 mg/day.13 In addition, the pooled analysis was based on 6 heterogeneous cohorts with substantial differences in baseline characteristics of participants, and generalizability of these findings to the overall US population is unclear. Since the publication of that article, results from several other cohort studies were published and the inconsistent findings further contributed to the ongoing debates on the relationship between dietary eggs and cholesterol and CVD.14, 15, 16, 17 Nevertheless, a comprehensive assessment of the associations in a cohort of US representative population is still needed and may provide valuable evidence for establishment of a general dietary guideline for adults.
In view of these circumstances, we used data from the US National Health and Nutrition Examination Survey (NHANES) to examine dietary intakes of eggs and cholesterol in relation to all‐cause and heart disease mortality. We also sought to determine whether such associations vary at different consumption levels and in different subgroups of the population.
Methods
Study Population
All data were obtained from the NHANES, a complex, multistage, probability sampling designed survey to assess the health and nutritional status of adults and children in the United States.18 The data are publicly available and can be accessed at https://wwwn.cdc.gov/nchs/nhanes/default.aspx. Detailed descriptions of the planning and operation of each survey have been reported elsewhere.18 The methods used in the analysis that support the findings of this study are available from the corresponding author upon reasonable request.
A total of 43 793 adults ≥20 years of age were interviewed in 8 cycles (1999–2014) of the NHANES. For the current analyses, we excluded participants with implausible caloric intake (<500 kcal or >6000 kcal/day)11 or incomplete dietary recalls (n=5073), pregnant or lactating women (n=1542), or when there was lack of mortality status (n=57). A total of 37 121 participants were included in the main analyses (Figure S1). The NHANES was approved by the ethics review board of National Center for Health Statistics Research, and written informed consent was obtained from each participant.
Dietary Assessment
Food and nutrient intakes were assessed via 24‐hour dietary recalls by trained interviewers using the US Department of Agriculture Automated Multiple‐Pass Method at the Mobile Examination Center. A standard set of measuring guides was used to assist the respondent to report the volume and dimensions of the items. In the 1999–2002 cycle, a single in‐person dietary recall was conducted on a randomly chosen day, whereas, beginning in 2003, a second dietary recall was collected by telephone interview approximately 3 to 10 days after the first one. Food intakes were coded and nutrient values were determined according to the US Department of Agriculture Food and Nutrient Database for Dietary Studies, versions 1.0 to 5.0.19 To account for within‐respondent random measurement error and provide estimated dietary intakes over long periods, the usual intakes of foods and nutrients were calculated using a statistical method developed at the National Cancer Institute.20, 21, 22 Consumption of food groups was considered using the Food Patterns Equivalents Database.23 Egg consumption was defined as intake of whole eggs or egg ingredient in mixed dishes, with the exception of egg substitutes.
Outcome Assessment
Vital status of the NHANES participants was ascertained by probabilistic matching with the National Death Index through December 31, 2015.24 The cause of death was determined according to the International Classification of Diseases, Tenth Revision (ICD‐10). Total deaths and deaths from heart disease (codes I00–I09, I11, I13, I20–I51) were primary outcomes in our analyses. Due to data availability, we only conducted an exploratory analysis for stroke mortality (codes I60–I69; 191 cases) among 16 556 participants enrolled in 1999–2006. Follow‐up time was calculated as person‐years from the date of interview to the date of death or December 31, 2015, whichever came first.
Covariate Assessment
Demographic information and lifestyle factors, including age, sex, race/ethnicity, education, family income, marital status, cigarette smoking, and physical activity, were collected at the baseline survey. Alcohol use, weight, and height were assessed during physical examination at the Mobile Examination Center. Body mass index (BMI) was calculated as weight in kilograms divided by height in meters squared. The family income–poverty ratio was defined as the total family income divided by the poverty threshold, and a lower ratio indicated lower socioeconomic status. Smokers were defined as participants who reported smoking >100 cigarettes during their lifetime and were current cigarette smokers, and former smokers were defined as those who had quit smoking before the time of the interview. Current smokers were further categorized into 3 groups: <10, 10 to 19, or ≥20 cigarettes per day. Alcohol drinkers were defined as those who consumed ≥12 alcoholic drinks in a given year, and further categorized into low‐to‐moderate drinkers (<1 [women] or <2 [men] drinks per day) or heavy drinkers (≥1 [women] or ≥2 [men] drinks per day). Diet quality was evaluated using the Healthy Eating Index‐2015, which assessed the adherence to the 2015–2020 Dietary Guidelines for Americans,25 with a higher score indicating better diet quality. Participants were categorized as having low, medium, and high levels of physical activity if they performed the equivalent of <150, 150 to 300, and >300 minutes of moderate‐intensity physical activity per week according to the 2018 Physical Activity Guidelines for Americans.26 Prevalent CVD was defined as self‐reported diagnosis of coronary heart disease, stroke, heart attack, or congestive heart failure, and prevalent cancer was defined as self‐reported physician diagnosis of cancer or a malignancy of any kind. Diabetes mellitus was defined as fasting glucose ≥7 mmol/L or glycated hemoglobin level ≥6.5%, or taking glucose‐lowering medications.27 Hypertension was defined as systolic/diastolic blood pressure ≥140/90 mm Hg or taking antihypertensive medications.28 Hypercholesterolemia was defined as total cholesterol ≥6.2 mmol/L or taking lipid‐lowering medications.29
Statistical Analysis
Among the 37 121 eligible participants, there were missing values for education level (n=48; 0.1%), family income–poverty ratio (n=2950; 7.9%), marital status (n=431; 1.2%), cigarette smoking (n=93; 0.3%), alcohol consumption (n=2239; 6.0%), physical activity (n=131; 0.4%), and BMI (n=628; 1.7%). Multiple imputation was done for all missing values.30
Characteristics of the participants across intake groups of eggs or dietary cholesterol were compared using analysis of variance for continuous variables and the Rao–Scott chi‐square test for categorical variables, accounting for sampling design. Cox proportional hazards models were used to examine the relationship between dietary intake of egg and cholesterol and risk of all‐cause and heart disease mortality. The proportional hazards assumption was tested only for the exposure variables by dividing the follow‐up period into 3 intervals that held similar numbers of deaths and comparing the associations using the Wald test, and no evidence of departure from the assumption was found. Given the recent inconsistency regarding the association of eggs and cholesterol with mortality risk, we hypothesized that these associations could be nonlinear and depend on level of intake. Therefore, we first used categorical exposures in the analysis, and egg intake was classified into 3 groups (<0.5, ≥0.5 to <1, and ≥1 egg per day), based on the consumption pattern that people usually eat eggs in a predefined unit, whereas dietary cholesterol intake was categorized according to quintiles. We also applied the Cox proportional hazards model based on restricted cubic splines to explore the nonlinear associations. In the presence of nonlinearity, the analysis using continuous exposure (daily increase of 0.5 egg or 50 mg cholesterol) with mortality risk was stratified by intake subgroup levels based on the turning point on the spline curve. The hazard ratios (HRs) and 95% CIs were estimated with adjustment for potential confounders in a stepwise manner. Model 1 was adjusted for age, sex, race/ethnicity, education, family income–poverty ratio, marital status, and survey cycles. Model 2 was also adjusted for total energy intake, cigarette smoking, alcohol use, and physical activity. Model 3 was further adjusted for BMI, baseline history of hypertension, diabetes mellitus, hypercholesterolemia, CVD, and cancer. Exploratory subgroup analyses were performed by age (<65 and ≥65 years of age), sex (male and female), race/ethnicity (non‐Hispanic white, non‐Hispanic black, Mexican American, and other), smoking status (never, former, and current smoker), BMI (<25.0, 25.0–29.9, and ≥30.0 kg/m2), and baseline history of comorbidities (yes and no). The significance of the effect modification was examined by the Wald test with addition of a cross‐product term of the potential effect modifier and continuous exposure variable to the final model.
We conducted a series of sensitivity analyses to test the robustness of the results, including: (1) additional adjustment for nutrients correlated with dietary cholesterol (saturated fat, total fat, fiber, and sodium) individually or in combination, in addition to covariates in model 3; (2) additional adjustment for cholesterol‐containing foods (unprocessed red meat, processed meat, poultry, seafood, and total dairy products) individually or in combination, in addition to covariates in model 3; (3) mutual adjustment for eggs and dietary cholesterol in model 3; (4) additional adjustment for the Healthy Eating Index‐2015 in model 3; (5) excluding deaths within 2 years of follow‐up; (6) censoring at 10‐year follow‐up; and (7) excluding participants with major diseases at baseline, such as CVD and cancer. All statistical values were adjusted using sample weights in the NHANES to account for the complex survey design and oversampling of certain subgroups as specified in the instructions.18 Data analyses were performed using SAS version 9.4 (SAS Institute, Cary, NC). Statistical significance was defined as two‐sided P<0.05.
Results
Baseline Characteristics
The mean age (SD deviation) of the 37 121 participants was 50.2 (18.1) years, and 49.2% were men. The mean intakes (standard deviation) of eggs and cholesterol were 0.6 (0.3) egg per day and 288.3 (85.9) mg/day, respectively. The distributions of dietary intakes of eggs and cholesterol are plotted in the Figures S2 and S3.
Compared with participants who consumed <0.5 egg per day, those who consumed >1 egg per day were older and had a lower education level and lower family income–poverty ratio, but had higher BMI and physical activity levels. They were also more likely to be male, non‐Hispanic black, Mexican American, married, a former smoker, a low‐to‐moderate alcohol drinker, and have prevalent medical conditions (Table 1). Participants with a higher egg consumption rate also had higher intakes of total energy and dietary cholesterol, but slightly lower Healthy Eating Index‐2015 scores. Characteristics patterns for participants with high vs low intakes of dietary cholesterol were generally similar to those for egg consumption, but participants with high dietary cholesterol intakes were younger and less likely to have hypertension, hypercholesterolemia, CVD, and cancer (Table S1). Correlation coefficients between dietary intakes of eggs and cholesterol, and selected nutrients and foods are presented in Table S2. Overall, there were significant and positive correlations between dietary cholesterol intake and certain nutrients, including total and saturated fat and sodium, and foods, including eggs, unprocessed red meat, processed meat, poultry, and seafood (Pearson correlation coefficient ≥0.18 for all).
Table 1.
Baseline Characteristics of Study Participants Across Egg Consumption Groups in the National Health and Nutrition Examination Survey 1999–2014
| Characteristicsa | Total (n=37 121) | No. of Eggs per Day | P Value | ||
|---|---|---|---|---|---|
| <0.5 (n=16 501) | 0.5– 1 (n=16 818) | >1 (n=3802) | |||
| Age, y | 50.2±18.1 | 47.8±18.3 | 51.8±17.9 | 53.9±17.1 | <0.001 |
| Sex | |||||
| Male | 18 634 (49.2) | 6368 (39.8) | 9253 (55.7) | 3013 (79.6) | <0.001 |
| Female | 18 487 (50.8) | 10 133 (60.2) | 7565 (44.3) | 789 (20.4) | |
| Race/ethnicity | |||||
| Non‐Hispanic white | 17 657 (70.2) | 9127 (74.8) | 7310 (66.8) | 1220 (56.3) | <0.001 |
| Non‐Hispanic black | 7683 (11.1) | 2816 (8.6) | 3871 (13.1) | 996 (17.2) | |
| Mexican American | 6584 (7.9) | 2027 (5.6) | 3373 (9.2) | 1184 (16.5) | |
| Other | 5197 (10.8) | 2531 (11.0) | 2264 (10.8) | 402 (10.1) | |
| Education | |||||
| Less than high school | 10 476 (18.4) | 4060 (16.6) | 5053 (19.8) | 1363 (23.7) | <0.001 |
| High school | 8689 (24.2) | 3982 (24.9) | 3857 (23.4) | 850 (24.0) | |
| Some college or above | 17 908 (57.4) | 8439 (58.5) | 7887 (56.9) | 1582 (52.4) | |
| Family income–poverty ratio level | |||||
| 0 to 1.0 | 6940 (14.6) | 3120 (15.1) | 3059 (13.6) | 761 (16.2) | 0.01 |
| 1.1 to 3.0 | 14 255 (35.9) | 6221 (35.6) | 6529 (36.2) | 1505 (36.2) | |
| >3.0 | 12 976 (49.6) | 5884 (49.4) | 5865 (50.2) | 1227 (47.6) | |
| Marital status | |||||
| Married | 21 887 (62.1) | 9359 (60.5) | 10 062 (63.3) | 2466 (67.1) | <0.001 |
| Separated | 8418 (19.3) | 3704 (19.0) | 3909 (20.0) | 805 (17.9) | |
| Never married | 6385 (18.6) | 3238 (20.5) | 2642 (16.7) | 505 (15.0) | |
| Smoking status | |||||
| Never | 19 539 (52.2) | 9067 (53.4) | 8700 (51.3) | 1772 (48.7) | <0.001 |
| Former | 9446 (25.0) | 3696 (22.5) | 4558 (27.2) | 1192 (30.7) | |
| Current <10 cigarettes/day | 3327 (8.2) | 1451 (8.4) | 1485 (7.8) | 391 (8.8) | |
| Current ≥10 to <20 cigarettes/day | 2139 (6.1) | 1014 (6.8) | 938 (5.6) | 187 (4.5) | |
| Current ≥20 cigarettes/day | 2577 (8.5) | 1231 (8.9) | 1099 (8.1) | 247 (7.4) | |
| Drinking status | |||||
| Never | 10 086 (24.8) | 4783 (26.2) | 4526 (24.0) | 777 (19.2) | <0.001 |
| Low to moderate | 21 672 (64.5) | 9337 (62.9) | 9869 (65.6) | 2466 (70.0) | |
| Heavy | 3124 (10.7) | 1378 (11.0) | 1409 (10.4) | 337 (10.7) | |
| Physical activity | |||||
| Low level | 17 453 (42.3) | 7643 (42.3) | 8027 (42.4) | 1783 (41.3) | 0.04 |
| Moderate level | 4756 (13.8) | 2233 (14.4) | 2099 (13.5) | 424 (11.9) | |
| High level | 14 781 (43.9) | 6566 (43.3) | 6633 (44.1) | 1582 (46.8) | |
| Total energy, kcal/day | 2071.2±522.5 | 1969.1±498.5 | 2113.6±516.8 | 2326.6±536.3 | <0.001 |
| Total dietary cholesterol intake, mg/day | 288.3±85.9 | 239.7±60.1 | 305.2±67.2 | 423.9±80.8 | <0.001 |
| Healthy Eating Index‐2015 | 53.1±13.3 | 53.7±13.9 | 52.9±12.9 | 52.0±11.8 | 0.01 |
| Body mass index, kg/m2 | 28.8±6.7 | 28.7±6.9 | 28.8±6.6 | 29.1±6.1 | <0.001 |
| Medical conditions | |||||
| Hypertension | 13 767 (31.7) | 5491 (28.3) | 6679 (35.0) | 1597 (36.9) | <0.001 |
| Diabetes mellitus | 5503 (10.6) | 2007 (8.4) | 2692 (12.4) | 804 (15.8) | <0.001 |
| Hypercholesterolemia | 10 513 (27.5) | 4379 (25.5) | 4931 (29.4) | 1203 (31.4) | <0.001 |
| Cardiovascular disease | 4255 (8.9) | 1636 (7.5) | 2067 (10.2) | 552 (12.2) | <0.001 |
| Cancer | 3503 (9.5) | 1480 (8.7) | 1676 (10.3) | 347 (10.4) | <0.001 |
Data are presented as number (%) for categorical variables and mean (standard deviation) for continuous variables.
P values calculated by Rao–Scott chi‐square test for categorical variables, which is a design‐adjusted version of the Pearson chi‐square test; analysis of variance adjusting for sampling weights was used to calculate P values for continuous variables.
Association Between Egg and Dietary Cholesterol and Mortality Risk
During 290 698 person‐years of follow‐up (median, 7.8 years), there were 4991 deaths, including 870 deaths from heart disease. No significant associations were found between egg consumption and either all‐cause mortality or heart disease mortality (P≥0.18 for overall association, and P=0.13 for nonlinearity on the basis of restricted cubic splines; Figure). Consistently, no significant association was observed between each additional 0.5 egg per day and all‐cause mortality (HR, 1.04; 95% CI, 0.96–1.13) or heart disease mortality (HR, 0.96; 95% CI, 0.80–1.14; Table 2). There was no evidence of a significant association for all‐cause and heart disease mortality when egg consumption was evaluated categorically, comparing those consuming ≥1 vs ≤0.5 egg per day (P≥0.47 for trend; Table S3). Results remained stable after additional adjustment for various nutrients (total dietary cholesterol, saturated fat, total fat, fiber, and sodium), potential cholesterol‐containing foods, and Healthy Eating Index‐2015 score. Excluding events within the first 2 years, censoring at 10 years of follow‐up, or excluding participants with baseline CVD did not appreciably change the results (Table 2). Similarly, exploratory analyses also did not identify a significant association between egg consumption and stroke mortality (HR, 0.87; 95% CI, 0.58–1.31; Table S4).
Figure 1. Associations between dietary intakes of egg and dietary cholesterol and all‐cause and heart disease mortality.
A, Association between dietary intake of egg and risk of all‐cause mortality. B, Association between dietary intake of egg and risk of heart disease mortality. C, Association between dietary intake of cholesterol and risk of all‐cause mortality. D, Association between dietary intake of cholesterol and risk of heart disease mortality. The solid lines and shaded areas represent the central risk estimates and 95% CIs, respectively, relative to the reference level (0.25 egg consumed per day for A and B; 10th percentile for C and D). The dotted vertical lines correspond to 0.5, 1, 1.5, 2, and 2.5 eggs consumed per day for (A and B), and the 20th, 40th, 60th, and 80th percentiles of dietary cholesterol intake for (C and D).
Table 2.
Associations Between Each Additional 0.5 Egg Consumed per Day and All‐Cause and Heart Disease Mortality
| Modelsa | Adjusted Hazard Ratio (95% CI) | |
|---|---|---|
| All‐Cause Mortality | Heart Disease Mortality | |
| Model 1 | 1.04 (0.96–1.12) | 0.95 (0.81–1.11) |
| Model 2 | 1.05 (0.97–1.14) | 0.98 (0.84–1.16) |
| Model 3 | 1.04 (0.96–1.13) | 0.96 (0.80–1.14) |
| Model 3 plus nutrients | ||
| Total cholesterol | 0.97 (0.85–1.10) | 0.88 (0.66–1.17) |
| Saturated fat | 1.04 (0.95–1.13) | 0.96 (0.80–1.14) |
| Total fat | 1.05 (0.96–1.15) | 0.95 (0.79–1.14) |
| Fiber | 1.01 (0.93–1.10) | 0.94 (0.79–1.12) |
| Sodium | 1.05 (0.96–1.14) | 0.96 (0.81–1.14) |
| All previous nutrients | 0.97 (0.86–1.10) | 0.86 (0.65–1.15) |
| Model 3 plus cholesterol‐containing foods | ||
| Unprocessed red meat | 1.04 (0.95–1.13) | 0.95 (0.80–1.13) |
| Processed meat | 1.04 (0.96–1.14) | 0.96 (0.80–1.14) |
| Poultry | 1.04 (0.95–1.13) | 0.95 (0.80–1.13) |
| Seafood | 1.04 (0.96–1.14) | 0.96 (0.81–1.14) |
| Total dairy | 1.04 (0.96–1.13) | 0.93 (0.78–1.11) |
| All previous foods | 1.04 (0.96–1.13) | 0.93 (0.78–1.10) |
| Model 3 plus Healthy Eating Index‐2015 | 1.03 (0.94–1.12) | 0.95 (0.80–1.13) |
| Model 3 with restrictions | ||
| Excluding events within first 2 years | 1.03 (0.93–1.13) | 0.95 (0.78–1.16) |
| Censoring at 10‐year follow‐up | 1.07 (0.98–1.17) | 1.00 (0.81–1.24) |
| Excluding patients with baseline cardiovascular disease | 1.03 (0.92–1.15) | 0.94 (0.73–1.22) |
Complex survey designs are considered for all estimates. Model 1 was adjusted for age, sex, race/ethnicity, education, family income–poverty ratio, marital status, and National Health and Nutrition Examination Survey cycles. Model 2 was adjusted for total energy intake, cigarette smoking, alcohol drinking, physical activity, and covariates in model 1. Model 3 was adjusted for body mass index, baseline hypertension, diabetes mellitus, hypercholesterolemia, cardiovascular disease, cancer, and covariates in model 2.
Overall, dietary cholesterol intake was associated with all‐cause mortality (P=0.01 for overall association, based on the restricted cubic splines), but not with heart disease mortality (P=0.35 for overall association; Figure). In addition, the association was U‐shaped for all‐cause mortality (P=0.01 for nonlinearity). We separately assessed the association with all‐cause mortality in participants who consumed <250 mg/day cholesterol and in those who consumed ≥250 mg/day cholesterol. The 250‐mg/day cut‐off was approximately the turning point of the U‐shaped association and the median level of previous recommended upper limit of dietary cholesterol (ranging from 200 to 300 mg). Every 50‐mg/day increase of dietary cholesterol was inversely associated with all‐cause mortality among participants with daily cholesterol intake <250 mg (HR, 0.87; 95% CI, 0.77–0.98; Table 3), but positively associated with all‐cause mortality among participants with daily cholesterol intake ≥250 mg (HR, 1.07; 95% CI, 1.01–1.12). No significant association was found between dietary cholesterol intake and heart disease mortality (HR, 1.00 per 50 mg/day; 95% CI, 0.93–1.08; Table 4). On a consistent basis, there was no evidence of a linear association with all‐cause and heart disease mortality when dietary cholesterol intake was assessed in quintiles (P≥0.13 for linear trend for both; Table S5). For all‐cause mortality, the overall associations were generally similar in various sensitivity analyses in the 2 subgroups of participants on the basis of daily cholesterol intake levels (Table 3). In addition, the association between dietary cholesterol intake and heart disease mortality remained insignificant in all sensitivity analyses (Table 4). Our exploratory analyses also did not show a significant association between dietary cholesterol intake and stroke mortality (Table S6).
Table 3.
Associations Between Each Additional 50 mg of Dietary Cholesterol Consumed per Day and All‐Cause Mortality
| Modelsa | Hazard Ratio (95% CI) | |
|---|---|---|
| Daily Consumption <250 mg | Daily Consumption ≥250 mg | |
| Model 1 | 0.90 (0.80–1.02) | 1.04 (0.99–1.08) |
| Model 2 | 0.91 (0.80–1.03) | 1.07 (1.02–1.12) |
| Model 3 | 0.87 (0.77–0.98) | 1.07 (1.01–1.12) |
| Model 3 plus nutrients | ||
| Saturated fat | 0.87 (0.77–0.99) | 1.06 (1.00–1.12) |
| Total fat | 0.89 (0.78–1.01) | 1.07 (1.01–1.13) |
| Fiber | 0.83 (0.73–0.94) | 1.06 (1.00–1.11) |
| Sodium | 0.89 (0.78–1.00) | 1.07 (1.02–1.13) |
| All previous nutrients | 0.86 (0.75–0.98) | 1.05 (0.99–1.12) |
| Model 3 plus cholesterol‐containing foods | ||
| Egg | 0.90 (0.79–1.04) | 1.07 (1.00–1.14) |
| Unprocessed red meat | 0.85 (0.75–0.96) | 1.07 (1.01–1.12) |
| Processed meat | 0.87 (0.77–0.99) | 1.07 (1.01–1.13) |
| Poultry | 0.88 (0.77–1.00) | 1.07 (1.02–1.12) |
| Seafood | 0.87 (0.77–0.99) | 1.07 (1.02–1.13) |
| Total dairy | 0.87 (0.77–0.98) | 1.07 (1.02–1.12) |
| All previous foods | 0.94 (0.79–1.11) | 1.11 (1.04–1.20) |
| Model 3 plus Healthy Eating Index‐2015 | 0.85 (0.75–0.96) | 1.06 (1.01–1.12) |
| Model 3 with restrictions | ||
| Excluding events within first 2 y | 0.88 (0.76–1.01) | 1.05 (0.99–1.11) |
| Censoring at 10‐year follow‐up | 0.95 (0.82–1.10) | 1.09 (1.04–1.14) |
| Excluding patients with baseline cardiovascular disease | 0.84 (0.70–1.00) | 1.08 (1.02–1.14) |
Complex survey designs are considered for all estimates. Model 1 was adjusted for age, sex, race/ethnicity, education, family income–poverty ratio, marital status, and National Health and Nutrition Examination Survey cycles. Model 2 was adjusted for total energy intake, cigarette smoking, alcohol drinking, physical activity, and covariates in model 1. Model 3 was adjusted for body mass index, baseline hypertension, diabetes mellitus, hypercholesterolemia, cardiovascular disease, cancer, and covariates in model 2.
Table 4.
Associations Between Each Additional 50 mg of Dietary Cholesterol Consumed Per Day and Heart Disease Mortality
| Modelsa | Hazard Ratio (95% CI) |
|---|---|
| Model 1 | 0.97 (0.91–1.04) |
| Model 2 | 1.02 (0.95–1.09) |
| Model 3 | 1.00 (0.93–1.08) |
| Model 3 plus nutrients | |
| Saturated fat | 1.01 (0.93–1.09) |
| Total fat | 1.00 (0.92–1.08) |
| Fiber | 0.99 (0.92–1.08) |
| Sodium | 1.01 (0.93–1.08) |
| All previous nutrients | 1.00 (0.92–1.09) |
| Model 3 plus cholesterol‐containing foods | |
| Egg | 1.05 (0.93–1.19) |
| Unprocessed red meat | 0.99 (0.92–1.06) |
| Processed meat | 1.00 (0.93–1.08) |
| Poultry | 1.01 (0.93–1.09) |
| Seafood | 1.01 (0.93–1.08) |
| Total dairy | 1.00 (0.93–1.08) |
| All previous foods | 1.07 (0.92–1.25) |
| Model 3 plus Healthy Eating Index‐2015 | 1.00 (0.93–1.07) |
| Model 3 with restrictions | |
| Excluding events within first 2 years | 1.00 (0.92–1.08) |
| Censoring at 10‐year follow‐up | 1.01 (0.92–1.12) |
| Excluding patients with baseline cardiovascular disease | 1.03 (0.92–1.14) |
Complex survey designs are considered for all estimates. Model 1 was adjusted for age, sex, race/ethnicity, education, family income–poverty ratio, marital status, and National Health and Nutrition Examination Survey cycles. Model 2 was adjusted for total energy intake, cigarette smoking, alcohol intake, physical activity, and covariates in model 1. Model 3 was adjusted for BMI, baseline hypertension, diabetes mellitus, hypercholesterolemia, cardiovascular disease, cancer, and covariates in model 2.
There was little evidence of effect modifications for most stratifying variables (Figures S4 through S8). There seemed to be sex differences in the associations between dietary intake of eggs and all‐cause mortality: a positive association was observed in men (HR, 1.13; 95% CI, 1.01–1.27), whereas an inverse association was found in women (HR, 0.86; 95% CI, 0.75–0.99; P=0.01 for interaction; Figure S4). A similar pattern was found for the association between dietary cholesterol and all‐cause mortality for participants with cholesterol intake <250 mg/day (men: HR, 1.12; 95% CI, 0.89–1.42; women: HR, 0.82; 95% CI, 0.71–0.95; P=0.02 for interaction; Figure S6). The associations between dietary intake of eggs and all‐cause and heart disease mortality differed by baseline hypercholesterolemia status; that is, there seemed to be increased risks in participants with hypercholesterolemia but decreased risk in those without hypercholesterolemia (P≤0.03 for interaction; Figures S4 and S5). There were similar opposite associations between dietary cholesterol intake and heart disease mortality in subgroups stratified by hypercholesterolemia (P=0.002 for interaction; Figure S8), but such opposite associations for all‐cause mortality were restricted to participants with <250 mg/day dietary cholesterol (P=0.04 for interaction; Figure S6), but not among those with ≥250 mg/day dietary cholesterol (P=0.29 for interaction; Figure S7).
Discussion
In this study of 37 121 US adults with a median follow‐up of 7.8 years, we did not find significant associations between egg intake and all‐cause or heart disease mortality. No significant association was observed between dietary cholesterol and heart disease mortality. The association between dietary cholesterol and all‐cause mortality was nonlinear and the positive association was only observed among participants with daily cholesterol intake ≥250 mg.
Interpretation and Comparison With Previous Studies
As a major source of dietary cholesterol, the association between egg consumption and health outcomes has attracted substantial attention. Several meta‐analyses showed no significant associations between egg consumption and cardiovascular events or all‐cause mortality.6, 7, 8 For example, the most recent meta‐analysis of 9 prospective studies did not demonstrate evidence of association with all‐cause mortality (HR, 1.09; 95% CI, 0.997–1.20) or heart disease mortality (HR, 0.97; 95% CI, 0.90–1.05) for egg consumption of ≥1/day vs <1/week.8 Overall, our findings are consistent with those studies. Most studies in the meta‐analysis reported no significant association with all‐cause mortality, whereas the Physicians’ Health Study with 21 327 US men reported a positive association (HR, 1.23, comparing ≥1/day vs <1/week; 95% CI, 1.11–1.36),31 and the NIPPON DATA 90 study among Japanese women reported a positive association (HR, 2.05, comparing ≥2/day vs 1/day; 95% CI, 1.20–3.52).32 In addition, most of these studies reported no significant associations with incident CVD, CVD mortality, or heart disease mortality. A recent large‐cohort study with 461 213 Chinese adults reported a linear inverse association for incident CVD (including all CVD subtypes) and CVD mortality.33 However, a recent pooled analysis of 6 US cohorts indicated that each additional 0.5 egg consumed per day was significantly associated with higher risk of incident CVD (HR, 1.06; 95% CI, 1.03–1.10) and all‐cause mortality (HR, 1.08; 95% CI, 1.04–1.11).11 Despite the large sample size (n=29 615) and long‐term follow‐up (median, 17.5 years), this study may be subject to several limitations: (1) baseline assessments from included cohort studies spanned from 1985 to 2005, but the secular changes of egg consumption over the years were not taken into account; (2) baseline characteristics varied substantially in individual cohorts in terms of age, race/ethnicity, education level, smoking status, mean BMI, and health status (diabetes mellitus, hypertension, and blood lipid levels), as well as incidence and mortality rates; and (3) the 6 cohorts had different study designs and data collection procedures. Pooling individual data from these heterogeneous cohorts could be problematic if confounding patterns differed among studies, but effect sizes for individual studies were not estimated separately. In addition, the combined results may not be generalizable to the whole US population; therefore, our study has the unique advantage of representative sampling of the US population and standard and consistent data collection procedures. The inconsistent findings across previous studies may be because of different population characteristics, differential adjustments for confounders (such as lack of adjustment for other foods in certain studies), various egg consumption levels in different countries (eg, 25.5 g/day in 2011–2012 in the United States34 vs 16.2 g/day [rural] and 28.8 g/day in 2012 [urban] in China35), and different cooking methods for eggs (eg, mostly fried eggs in the United States, and boiled eggs or mixed dishes with vegetables in China). Taken together, the current evidence indicates that egg consumption of up to 1 per day should not be a concern for the general public, although relationships with very high intake levels (eg, ≥2/day) still need to be confirmed. However, the proportion of people with such a high intake level is small in the population.
In our study, we did not find a significant association between dietary cholesterol intake and heart disease mortality. The results are consistent with those of several previous studies on CVD mortality and also in a systematic review of 19 studies with 361 923 participants, which did not identify significant associations with incident coronary heart disease, ischemic stroke, or hemorrhagic stroke.36 Although increased dietary cholesterol intake was thought to modestly increase serum total and low‐density lipoprotein (LDL) cholesterol as well as LDL/high‐density lipoprotein (HDL) cholesterol ratio,37 such an effect may not substantially increase the risk of CVD. This was demonstrated in a meta‐analysis of 17 trials, where 100 mg/day dietary cholesterol only increased total cholesterol by 0.056 mmol/L (2.2 mg/dL) and the LDL/HDL cholesterol ratio by 0.020 unit.37 Cholesterol homeostasis is maintained constant through a compensatory mechanism,38 and increased intake of dietary cholesterol is associated with decreased synthesis of endogenous cholesterol. In the pooling study of 6 US cohorts, Zhong et al11 reported positive and monotonic associations between dietary cholesterol intake and risk of all‐cause mortality and incident CVD, even after adjustment for saturated fat, red meat, and other nutrients or foods consistently found to be related to risk of CVD and premature death.39, 40, 41, 42 However, we observed a nonlinear U‐shaped association between dietary cholesterol and all‐cause mortality, and the weakly positive association was only restricted to participants with a daily intake ≥250 mg. The result raises concerns over whether the association between dietary cholesterol and increased all‐cause mortality depends on the baseline cholesterol consumption levels. Although our results among individuals with high consumption levels are in part consistent with those of Zhong et al,11 there is still a possibility of residual confounding from foods and nutrients that may increase risk of mortality. Our findings need to be validated in other similar prospective studies to achieve consensus about the optimal intake levels of dietary cholesterol.
The findings for all‐cause and heart disease mortality were generally consistent in subgroups according to demographic characteristics, risk factors, and baseline medical conditions. In a recent study among 6 cohorts, the association between dietary cholesterol intake and all‐cause mortality was significantly stronger among women than men.11 However, our study showed opposite associations between egg consumption (and lower dietary cholesterol) and all‐cause mortality among men and women. Egg consumption was associated with higher risk in men, consistent with some earlier reports from the United States43, 44 and Sweden.45 Some studies suggested that dietary intake of eggs was associated with higher risk of CVD mortality among participants with diabetes mellitus,6, 7 but our study did not show any significant association between intake of eggs or cholesterol and mortality outcomes in patients with diabetes mellitus. However, we found divergent associations with all‐cause and heart disease mortality in participants with and without hypercholesterolemia. The higher risk observed among participants with hypercholesterolemia may imply that dietary cholesterol needs to be controlled in individuals with high blood cholesterol levels, whereas, in those without hypercholesterolemia, the inverse associations may suggest that dietary cholesterol intake is not a concern. Of note, a feeding trial identified limited rise in LDL cholesterol levels among hypercholesterolemic participants after feeding with 2 eggs per day, which was also assumed to be similar to that reported among normocholesterolemic participants.46 Because we conducted multiple exploratory subgroup analyses, positive results in the stratified analyses should be interpreted with caution.
Strengths and Limitations
Because the NHANES has a large sample size with systematic surveys of dietary factors and a representative sampling of the US population, our analyses can facilitate quantitative delineation of the impact of dietary intake of eggs and cholesterol in the US population. However, there are certain limitations to be acknowledged. First, the 24‐hour dietary recalls may not represent long‐term exposure levels and participants may have changed their dietary habits during the follow‐up. Second, although we adjusted for certain lifestyle and dietary factors, residual confounding cannot be entirely dismissed. Third, we could not examine the associations of dietary intake of eggs and cholesterol with incident CVD and their subtypes because of the lack of available data. Fourth, the average follow‐up was 7.6 years, which may not be long enough for assessing the long‐term dietary impact on mortality outcomes in a relatively young population. Fifth, information was available on apolipoprotein E gene polymorphism, which has been shown to affect variations of serum cholesterol in response to dietary cholesterol43; therefore, further study is needed to determine whether the association between dietary cholesterol intake and health outcomes can be modified by genetic background.47, 48
Conclusions
In this study we did not find a significant association between dietary intake of eggs and all‐cause and heart disease mortality in US adults, whereas total dietary cholesterol intake at high levels seemed to be associated with higher risk of all‐cause mortality. Our findings are generally supportive of the recommendation from the 2015–2020 Dietary Guidelines for Americans that moderate consumption of up to 1 egg per day may be included in a healthy dietary pattern. However, caution should be taken for high‐risk individuals, such as those with hypercholesterolemia and those already with high dietary cholesterol intake levels. Our analyses should be replicated in other populations with different intake levels of eggs and cholesterol. In addition, further prospective studies are warranted to investigate the relationships between of high dietary egg and cholesterol intake and other major health outcomes such as incident CVDs and their subtypes.
Sources of Funding
This study was supported by the National Key Research and Development Program of China (grant no. 2017YFC0907504 to A.P.); the National Natural Science Foundation of China (grant no. 81930124 to A.P.); the China Postdoctoral Science Foundation (grant no. 176596 to X.‐F.P.); and the International Postdoctoral Exchange Fellowship of the China Postdoctoral Council (grant no. 20180062 to X.‐F.P.). The funders had no role in design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, and approval of the manuscript; or the decision to submit the manuscript for publication.
Disclosures
None.
Supporting information
Tables S1–S6 Figures S1–S8
(J Am Heart Assoc. 2020;9:e015743 DOI: 10.1161/JAHA.119.015743.)
For Sources of Funding and Disclosures, see page 10.
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Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Supplementary Materials
Tables S1–S6 Figures S1–S8