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. 2020 Oct 26;9:e55865. doi: 10.7554/eLife.55865

Figure 6. Inhibition of IRE1α decreases tumor cell proliferation.

Figure 6.

(A) PCNA mRNA-expression of HepG2 or Huh7-cells grown with LX2-cells in transwell inserts and treated with the IRE1α-inhibitor 4μ8C or control. (B) Relative cell number of LX2 and HepG2 or (C) LX2 and Huh7-cells treated with 4μ8C or control. (D) Representative images of tumor cells (HepG2 or Huh7) and LX2-stellate cells stained with antibodies against the HCC-marker EPCAM and the proliferation marker KI67. (E) Cell proliferation of HepG2 or HepG2+LX2 spheroids and (F) Huh7 or Huh7+LX2 spheroids treated with 4μ8C or control. p-Values were calculated via the Student's T-test from nine biological replicates per group, scale bars = 50 μm.