Table 3.

The effect of compounds 7a–k, when tested against two cancer cell lines, on the expression of genes related to apoptosis and the cell cycle.

BT-474
Compound	p21	p53	CD1
SAHA	17.39 ± 3.70 *	0.12 ± 0.08 *	0.02 ± 0.00 *
7c	3.81 ± 1.08 *	0.57 ± 0.27 *	0.51 ± 0.09 *
7d	1.47 ± 0.41	0.47 ± 0.08 *	0.92 ± 0.06
7e	5.47 ± 2.01 *	0.08 ± 0.00 *	0.57 ± 0.07 *
7f	23.65 ± 6.69 *	0.51 ± 0.22 *	0.40 ± 0.36
7i	0.44 ± 0.02 *	0.74 ± 0.21	0.91 ± 0.05
7j	26.56 ± 0.44 *	0.57 ± 0.24 *	0.32 ± 0.09 *
PC3
Compound	p21	p53	CD1
SAHA	7.44 ± 2.24 *	0.12 ± 0.14 *	0.04 ± 0.01 *
7c	3.48 ± 0.55 *	0.25 ± 0.17 *	0.61 ± 0.13
7d	1.41 ± 0.27	0.49 ± 0.23 *	0.64 ± 0.19
7e	3.90 ± 0.43 *	0.18 ± 0.11 *	0.30 ± 0.02 *
7f	3.60 ± 0.78 *	0.16 ± 0.20 *	0.32 ± 0.22 *
7i	2.07 ± 0.66	0.42 ± 0.39	0.90 ± 0.30
7j	3.80 ± 2.33	0.16 ± 0.06 *	0.43 ± 0.27 *

BT-474 and PC3 cells were incubated in the presence (10 μM) or absence of SAHA or compounds 7c–f, i–j for 24 h. Subsequently, mRNA was extracted, and real-time PCR (qPCR) was performed. Data are expressed as the mean ± SD of the genes/GAPDH, using the normalized ratio of triplicate determinations for mRNA from at least two different experiments. Vehicle-treated cells were arbitrarily given a value of one. * p < 0.05 vs. vehicle.