Table 2.
Effect of methanol extract of T. leucostaphylum leaves and its derived fractions on castor oil induced diarrhea in mice.
| Dose Treatment (mg/kg, p.o) |
Onset Diarrhea (min) | Total No of Dry Faces | Total No of Wet Faces | Weight (mg) of Dry Faces | Weight (mg) of Wet Faces | % Inhibition of Defecation |
|---|---|---|---|---|---|---|
| Control | 14.17 ± 1.12 | 9.92 ± 0.34 | 6.92 ± 0.33 | 0.48 ± 0.002 | 0.37 ± 1.23 | − |
| Loperamide 3 | 93.39 ± 2.38 *** | 3.6 ± 0.23 *** | 1.7 ± 0.24 *** | 0.13 ± 0.08 ** | 0.09 ± 0.07 * | 75.44% |
| NTL 100 | 54.50 ± 1.34 *** | 9.67 ± 0.76 | 3.63 ± 0.22 *** | 0.88 ± 0.02 | 0.53 ± 0.03 | 47.54% |
| NTL 200 | 66.16 ± 1.82 *** | 5.0 ± 1.63 *** | 3.0 ± 0.16 *** | 0.64 ± 0.16 | 0.36 ± 0.11 * | 56.64% |
| NTL 400 | 84.67 ± 2.27 *** | 4.0 ± 1.00 *** | 2.0 ± 0.18 *** | 0.72 ± 0.18 ** | 0.26 ± 0.08 *** | 71.09% |
| DTL 100 | 75.33 ± 0.56 *** | 2.33 ± 0.80 *** | 2.21 ± 0.02 *** | 0.33 ± 0.04 *** | 0.30 ± 0.02 *** | 68.06% |
| DTL 200 | 85.67 ± 1.66 *** | 1.6 ± 1.06 *** | 1.5 ± 0.05 *** | 0.21 ± 0.05 ** | 0.28 ± 0.11 * | 76.15% |
| DTL 400 | 99.5 ± 0.12 *** | 1.5 ± 0.95 *** | 0.83 ± 0.13 *** | 0.32 ± 0.13 ** | 0.21 ± 0.15 *** | 88.01% |
| BTL 100 | 50.5 ± 0.85 *** | 7.17 ± 0.50 * | 4.10 ± 0.13 | 0.78 ± 0.02 * | 0.51 ± 0.01 | 40.75% |
| BTL 200 | 66 ± 1.81 *** | 5.3 ± 0.73 *** | 3.50 ± 0.09 ** | 0.61 ± 0.09 | 0.37 ± 0.06 * | 49.42% |
| BTL 400 | 77.6 ± 1.39 *** | 4.17 ± 0.43 *** | 3.33 ± 0.02 *** | 0.51 ± 0.02 ** | 0.28 ± 0.15 * | 51.87% |
| MTL 100 | 47.00 ± 1.90 *** | 10.17 ± 0.48 | 4.23 ± 1.16 * | 1.25 ± 0.06 ** | 1.00 ± 0.05 * | 38.87% |
| MTL 200 | 54.34 ± 1.30 *** | 6.67 ± 1.60 ** | 3.83 ± 0.06 ** | 0.38 ± 0.06 * | 0.81 ± 0.05 | 44.65% |
| MTL 400 | 64.37 ± 2.40 *** | 6.16 ± 1.11 ** | 3.67 ± 0.06 *** | 0.60 ± 0.05 | 0.47 ± 0.12 ** | 46.96% |
Results are presented as Mean ± SEM (n = 6); One Way Analysis of Variance (ANOVA) followed by Dunnett’s Multiple Comparison Test; * p < 0.05, ** p < 0.01, *** p < 0.001 were considered significant compared to the control sample. NTL = n-hexane fraction of T. leucostaphylum, DTL = dichloromethane fraction of T. leucostaphylum, BTL = n-butanol fraction of T. leucostaphylum, MTL = methanol extract of T. leucostaphylum.