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ROS production induced by HME on HCT-116 cells. ROS levels were cytofluorimetrically evaluated after 24 h treatment by staining of the cells with DCFDA as reported in Methods. Data are compared to untreated cells (control). (A) Mean values ± SD of three separate experiments in triplicate. Means with different letters are significantly different (one-way Anova associated with Tukey’s post hoc test) with * p < 0.05; *** p < 0.001. (B) Representative images.
