Table 1.
Results of method validation.
| Compound | Sensitivity | Linearity | Precision and Accuracy | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Within-Run (n = 5) | Between-Run (n = 10) | ||||||||||
| LOD (ng/mL) | LQQ (ng/mL) | R2 | Slope tcal > tcri | Expected Conc. (ng/mL) | Measured Conc. (ng/mL) | Precision (CV%) | Accuracy (%) | Measured conc. (ng/mL) | Precision (CV%) | Accuracy (%) | |
| 3-NT | 0.030 | 0.100 | 0.997 | 168.18 > 1.94 | 0.275 1.5 2.5 |
0.261 ± 0.006 1.52 ± 0.07 2.54 ± 0.18 |
9.04 3.11 2.75 |
94.9 101.13 101.44 |
0.264 ± 0.005 1.49 ± 0.07 2.52 ± 0.15 |
7.26 3.21 2.35 |
96.0 99.63 100.99 |
| 3-CT | 0.026 | 0.096 | 0.996 | 175.39 > 1.94 | 0.275 1.5 2.5 |
0.279 ± 0.002 1.51 ± 0.11 2.61 ± 0.29 |
2.49 4.81 4.18 |
101.5 100.45 104.50 |
0.279 ± 0.002 1.52 ± 0.09 2.57 ± 0.24 |
2.51 4.05 3.59 |
101.5 101.63 102.84 |
| 3-BT | 0.030 | 0.098 | 0.996 | 170.82 > 1.94 | 0.268 1.5 2.5 |
0.239 ± 0.001 1.57 ± 0.09 2.52 ± 0.19 |
1.52 3.61 2.97 |
97.5 104.73 100.77 |
0.270 ± 0.002 1.54 ± 0.08 2.53 ± 0.15 |
2.10 3.21 2.30 |
98.3 102.88 101.21 |
3-NT, 3-Nitro-l-tyrosine; 3-CT, 3-Chloro-l-tyrosine; 3-BT, 3-Bromo-l-tyrosine; LOD, limit of detection; LOQ, limit of quantification; CV, coefficient of variation; R2, regression coefficient; conc., concentration.