Extended Data Figure 5. Physiological response properties of Tacr1⁺ and Gpr83⁺ SPB neurons.

a, b, Summary violin plots of peak instantaneous firing rates of Gpr83⁺ (a) and Tacr1⁺ (b) SPB neurons in response to von Frey indentations and thermal stimuli. Red lines indicate median, while blue lines indicate quartiles. Friedman test (Dunn’s multiple comparison test). n = 16, 15 neurons for Tacr1⁺, Gpr83⁺, respectively. c, Representative traces of action potential firing evoked by topical capsaicin (0.05%) treatment. Arrows, time when capsaicin was applied to the skin. d, Quantification of peak instantaneous firing rates upon capsaicin application. Mann-Whitney test (two-tailed); p value is indicated; n = 11, 7 neurons for Tacr1⁺, Gpr83⁺, respectively; error bars, s.e.m.