WNT signaling suppresses oligodendrogenesis via Ngn2-dependent direct inhibition of Olig2 expression

Abstract

Olig2 transcription factor is essential for the maintenance of neural progenitor cells (NPCs) in the pMN domain and their sequential specification into motor neurons (MNs) and oligodendrocyte precursor cells (OPCs). The expression of Olig2 rapidly declines in newly generated MNs. However, Olig2 expression persists in later-born OPCs and antagonizes the expression of MN-related genes. The mechanism underlying the differential expression of Olig2 in MNs and oligodendrocytes remains unknown. Here, we report that activation of WNT/β-catenin signaling in pMN lineage cells abolished Olig2 expression coupled with a dramatic increase of Ngn2 expression. Luciferase reporter assay showed that Ngn2 inhibited Olig2 promoter activity. Overexpression of Ngn2-EnR transcription repressor blocked the expression of Olig2 in ovo. Our results suggest that down-regulation of WNT-Ngn2 signaling contributes to oligodendrogenesis from the pMN domain and the persistent Olig2 expression in OPCs.

Olig2 is the key transcription factor that not only maintains the neural progenitor cells (NPCs) of pMN domain, but also regulates the sequential specification of NPCs into motor neurons (MNs) and OPCs [1–5]. Since persistent expression of Olig2 is inhibitory to post-mitotic MN genes [6], the expression of Olig2 rapidly declines in newly generated MNs, but remains high in later-born cells of oligodendrocyte lineage [2–6]. The mechanism of down-regulation of Olig2 expression in MNs remains elusive. WNT signaling is known to regulate the balance between the proliferation and differentiation of NPCs during neurogenesis [7]. It is interesting that endogenous WNT/β-catenin signaling is activated in newly generated MNs [8]. Activation of WNT/β-catenin signaling has been reported to inhibit the specification of OPCs and astrocytes from NPCs during early stages of gliogenesis [9–11]. However, the mechanism underlying the inhibition of OPC specification from pMN NPCs by WNT/β-catenin signaling remains to be determined.

Here, we utilized the Olig1^Cre/+;Ctnnb1^ΔEx3/+ transgenic mice to activate WNT signaling in the pMN domain. At embryonic day 12.5 (E12.5) when oligodendrogenesis commences, expression of Olig1 and Olig2 remains high in the pMN neural progenitor cells from which Pdgfra+ OPCs arise (Fig. 1a). Strikingly, in Olig1^Cre/+;Ctnnb1^ΔEx3/+ transgenic mice, activation of WNT signaling totally abolished the expression of Olig1, Olig2 and Pdgfra (Fig. 1a), indicating a complete inhibition of oligodendrogenesis. By contrast, the number of ISL1-positive MNs was only decreased slightly in Olig1^Cre/+;Ctnnb1^ΔEx3/+ mice (Additional file 1: Fig. S1), consistent with the previous finding that Olig1 is intermittently expressed in pMN NPCs and only weakly expressed during neurogenesis stage [12]. Although Olig1^Cre was also transcribed in P3 domain at early stages [13, 14], expression of P3 domain marker NKX2-2 was not suppressed in Olig1^Cre/+;Ctnnb1^ΔEx3/+ mice (Additional file 1: Fig. S1). However, the number of Ngn2-positive cells was dramatically increased within the ventral ventricular region in Olig1^Cre/+;Ctnnb1^ΔEx3/+ mice (Fig. 1a), demonstrating that WNT activation promotes Ngn2 expression. In support of this notion, overexpression of Ctnnb1^ΔEx3 in embryonic chicken spinal cord also caused an increase of Ngn2 expression, coupled with a reduced expression of Olig2 (Fig. 1d). At E18.5, although a few dorsally-derived [15–17] OPCs were generated from Olig1^Cre/+;Ctnnb1^ΔEx3/+ mice, Plp1-positive mature oligodendrocytes were still undetectable (Additional file 1: Fig. S2) since dorsal OPCs differentiate only after birth [14]. Together, these results strongly suggest that Ngn2 is the candidate gene that mediates the suppression of oligodendrogenesis from pMN NPCs by WNT signaling.

Fig. 1.

WNT signaling inhibit Olig2 expression through upregulation of Ngn2 expression. a Transverse sections of spinal cord at E12.5 from control and WNT signaling activated (Olig1^Cre/+;Ctnnb1^ΔEx3/+) mice were subjected to IF with anti-OLIG2 antibody or ISH with Olig1, Pdgfrα and Ngn2 riboprobes. The cells positive for OLIG2, Olig1 and Pdgfrα are absent in the spinal cord from Olig1^Cre/+;Ctnnb1^ΔEx3/+ mice, whereas Ngn2 is upregulated. Inset highlights the expression of OLIG2 in pMN domain, note that vascular development was abnormal in the spinal cord of Olig1^Cre/+;Ctnnb1^ΔEx3/+ mice. b There are putative Ngn2 binding sequences in the promoter regions of Ngn2 from human, rat and mouse. c Luciferase report assay revealed that Ngn2 but not its DNA binding deficient mutant AQ-Ngn2 inhibit the promoter activity of mouse Olig2. *p < 0.05, t-test. d Over-expression of Ngn2-EnR mimics the phenotype caused by over-expression of Ctnnb1-ΔEx3. Both expression of Ctnnb1-ΔEx3 and Ngn2-EnR suppressed the expression of OLIG2 in ovo. Arrowhead indicates induced expression of chick Ngn2 (cNgn2). Arrows represent reduced expression of endogenous genes. e OLIG2 maintains proliferation of pMN domain neural progenitor cells. High level of WNT signaling upregulates Ngn2 expression, NGN2 in turn coordinate with OLIG2 to promote motor neurons specification and suppress Olig2 expression in newly generated motor neurons. OPCs were specified OLIG2+ cells when WNT signaling is declined at the gliogenesis stage

In line with this concept, two NGN2 recognition sequences are identified in the upstream promoter of the Olig2 gene in human, rat and mouse (Fig. 1b). Luciferase reporter assay revealed that Ngn2 but not its DNA binding defective mutant AQ-Ngn2 can inhibit the promoter activity of mouse Olig2 (Fig. 1c), demonstrating that Ngn2 can bind to the promoter of Olig2 and repress its expression. To confirm that Ngn2 mediates WNT inhibition of oligodendrogenesis, we overexpressed Ngn2 in embryonic chicken spinal cord by in ovo electroporation and found a significant decrease of Olig2 and Pdgfra expression in the electroporated side at cE7 (Additional file 1: Fig. S3). Since Ngn2 can function either as a transcriptional activator or a repressor, we next investigated whether the inhibition of Olig2 expression is mediated by the transcriptional repressor activity of Ngn2. RCAS-Ngn2-EnR (DNA binding domain of NGN2 fused with EnR transcription repressor) was employed as a repressor-only NGN2 chimeric protein. It was found that overexpression of this chimeric repressor caused a significant reduction of Olig2 expression (Fig. 1d), mimicking the effect of full-length Ngn2 protein. This finding demonstrated that Ngn2 inhibits Olig2 expression by its transcriptional repressor activity.

In conclusion, our results suggest that WNT signaling up-regulates the expression of Ngn2, and Ngn2 in turn inhibits Olig2 expression and oligodendrogenesis during MN specification (Fig. 1e).

Authors’ contributions

ZMD conceived the project. MJ, DY, BX, HH, WL and ZMD performed the experiments. MJ, DY, BX, HH, MQ, WL and ZMD analyzed the data. MQ and ZMD supervised the project. MJ, MQ and ZMD wrote the paper with input from the other authors. All authors read and approved the final manuscript.

Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 31871480, 81771028, 31771621); the Natural Science Foundation of Zhejiang Province (Grant No. LY18C090009, LQ15C070001, LQ18C090005).

Availability of data and materials

All date generated during this study are included in this article.

Ethics approval and consent to participate

The use of animals was approved by the Committee on Laboratory Animals, Hangzhou Normal University.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no conflict of interest.

Supplementary information

Supplementary information accompanies this paper at 10.1186/s13041-020-00696-0.