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. 2020 Nov 15;47(12):9301–9311. doi: 10.1007/s11033-020-05990-7

Regulation of chlorogenic acid, flavonoid, and iridoid biosynthesis by histone H3K4 and H3K9 methylation in Lonicera japonica

Tianrui Liu 1,#, Jian Yang 1,#, Shuang Liu 1,#, Yuyang Zhao 1, Junhui Zhou 1, Yan Jin 1, Luqi Huang 1, Yuan Yuan 1,✉
PMCID: PMC7666716  PMID: 33190199

Abstract

Lonicera japonica is used in Chinese herbal medicines with a wide spectrum of pharmacological properties associated with chlorogenic acid, flavonoid and iridoid. The biosynthesis of these compounds could be affected by genetic inheritance and epigenetic modification. However, the mechanisms that regulate the expression of genes involved in the biosynthesis of these compounds are rarely known. The results of qRT-PCR showed that the biosynthesis gene expression of these compounds was related to histone H3K4 and H3K9 methylation levels. These active compounds content of L. japonica were measured by UPLC-MS/MS. H3K4me3 showed a positive correlation with chlorogenic acid and loganic acid content, and H3K9me positively correlated with luteolin content. The correlation between histone methylation levels and the levels of luteolin and loganic acid in L. japonica from different producing areas validate the regulatory role of histone methylation in biosynthesis of bioactive compounds. Our study demonstrated a potential regulatory network of H3K9/H3K4 methylation to gene expression and content of secondary metabolites, and provided a basis for understanding the mechanism underlying the variation of major bioactive compounds in L. japonica.

Electronic supplementary material

The online version of this article (10.1007/s11033-020-05990-7) contains supplementary material, which is available to authorized users.

Keyword: Bioactive compounds, Different producing areas, Histone methylation, Lonicera japonica

Introduction

Lonicera japonica is an important medicinal herb and its buds are used in the preparation of herbal tea in East-Asia including China, Japan, and Korea [1]. L. japonica buds are widely used to treat pancreatic cancer [2], H1N1 influenza infection [3], severe acute respiratory syndrome, and hand-foot-and-mouth disease [4].

The major bioactive components of L. japonica are chlorogenic acids, flavonoids and iridoids [5–7]. Chlorogenic acids possess anti-oxidative, anti-hypoxic, antibacterial, antiviral, and anti-inflammatory properties [8–15]. Luteoloside inhibits the risk of cardiovascular disease, severe acute respiratory syndrome [16], and hepatitis, and ameliorates intervertebral disk degeneration [17]. Other pharmaceutically active metabolites include iridoids and secoiridoids, and over 30 iridoids have been identified in L. japonica in the past decades [18, 19]. Loganin possesses anti-inflammatory and anti-shock effects [20–23].

Phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H), and 4-coumarate CoA ligase (4CL) are essential enzymes required for the initial steps of chlorogenic acid and luteoloside biosynthesis. Hydroxycinnamoyl-CoA quinate transferase (HQT) is a key enzyme acting downstream of the chlorogenic acid metabolic pathway [24]. Chalcone isomerase (CHI) is required for the conversion of naringenin chalcone to naringenin and is considered an essential enzyme for the biosynthesis of flavonoids [25] (Fig. S1A). Isopentenyl pyrophosphate (IPP) and dimethylallyl diphosphate triammonium salt (DMAPP) were produced by the mevalonate (MVA) and methylerythritol 4-phosphate (MEP) pathways. IPP and DMAPP condense to form geranyl pyrophosphate (GPP) in a reaction catalyzed by GPP synthase (GPPS). Geraniol synthase (GES) catalyzes the formation of geraniol from GPP. Geraniol is converted to 10-hydroxygeraniol by the geraniol-10-hydroxylase (G10H), and 10-hydroxygeraniol is converted to 10-oxogeranial by 8-hydroxygeraniol dehydrogenase (10HGO). Finally, 10-oxogeranial is converted to epi-iridodial by iridoid synthase (IS) [26] (Fig. S1B).

Lonicera japonica plantation accounts for more than 360,000 hectares of land in China, and the production of L. japonica flower buds is about 26,000 tons per year. In 2010, the domestic demand for L. japonica flower buds exceeded 80,000 tons, and the demand has increased annually since then. Analysis of gene transcript levels have shown that a variation in active compound content changes the pharmacological activities and medicinal qualities owing to artificial selection in germplasm, tissue, different growth stages, and pruning [27]. The synthesis and accumulation of chlorogenic acids and luteoloside at different growth stages and different tissues [28–30] in L. japonica also correlated with variations in the activities of enzymes involved in the metabolism of phenylalanine, which included PAL, C4H, and 4CL. The synthesis and accumulation of chlorogenic acid was directly affected by variations in HQT activity, and the synthesis of luteoloside was directly affected by variations in CHI activity. Pruning decreased HQT and CHI gene expression, which led to a decrease in chlorogenic acid and luteoloside content in the flower bud of L. japonica.

However, plant introduction is the most important factor affecting the variation of active compounds during cultivation. The north of China is a traditional L. japonica producing area, and seedlings from this region have been introduced to many places with different ecological characteristics. There is a crucial need to improve the chemical quality of L. japonica for population growth, and the change in active compounds after plant introduction is largely regulated by epigenetic modifications. There is growing evidence indicating that plants employ sophisticated epigenetic mechanisms to fine-tune their responses to environmental stress following plant introduction. Epigenetic processes and elements, including DNA methylation, histone modification, chromatin remodeling, and noncoding RNAs, are involved in plant responses to abiotic and biotic stresses [31]. A previous report has revealed the presence of several miRNAs involved in the regulation of fatty acid and flavonoid biosynthesis in different varieties of L. japonica from different regions [32]. However, epigenetic variations in the bud of L. japonica after plant introduction and their correlation with accumulation of chlorogenic acids, luteoloside, and iridoids remain largely unknown.

Histone modification, including histone methylation and demethylation, is one of the most common types of epigenetic regulation and has been widely studied. Histone modification regulates development and responses to biotic and abiotic stresses in plants [33]. The majority of histone methylation takes place on the lysine residue of histone H3 in which H3K4 and H3K9 are highly conserved epigenetic markers for gene activation [34, 35]. Histone methylation is dynamically regulated by histone methyltransferases (HMTs) and histone demethylases (HDMs) [31, 36]. In this study, we reported for the first time the potential mechanism of bioactive compound biosynthesis regulated by H3K4 and H3K9 methylation in L. japonica, and elucidated the correlation among transcriptional levels of HMTs and HDTs, histone methylation levels, and bioactive compounds of L. japonica. This work will provide a basis for understanding the epigenetic mechanism of bioactive compound synthesis and gene expression regulated by H3K4 and H3K9 methylation in L. japonica.

Material and methods

Plant materials

Fresh buds of L. japonica were collected from Yunnan, Beijing, Hebei, and Hubei provinces of China, and the seedlings in Yunnan, Hebei, and Hubei were introduced from Beijing. Fresh leaves of L. japonica were treated with 80 μM 5-azacytidine (5-azaC) for 48 h, while untreated leaves served as control. The treated and untreated leaves were from the same trees, and the leaves were stored at − 80 °C until use. All experiments were performed in triplicate.

Methods

RNA isolation, cDNA synthesis, and cloning

Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). An aliquot of 1 µg of total RNA was used to synthesize the first strand of cDNA using PrimeScript 1st Strand cDNA Synthesis Kit (Takara Bio, Dalian, China), according to the manufacturer’s protocol. Each cDNA was cloned using PrimeSTAR DNA polymerase (Takara Bio). Full-length cDNAs of HMTs and HDTs were cloned using PrimeSTAR® HS DNA polymerase (TaKaRa Biotechnology Co., Dalian, China) and ORF-PCR primers (Table 1).

Table 1.

PCR primers used for cloning full-length cDNAs of HMTs and HDTs

Primer name Primer sequence F (5′ → 3′) Primer sequence R (5′ → 3′)
HMT1 ATGATTTACGATTCCCTTAGGGTT TTAGTTACAAATGGGAAGCTTCCC
HMT2 ATGGGGAGCTCAGCTAACGGCTTC TCATTCTATGTAGTACTTGCTTTTG
HMT3 ATGGAGGTTCCTCAAGTTCAAAAGCA TCAATGGAGCTCAGCACCCGAACC
HMT4 ATGGAACAAGGAGTGGGTTCGGACT CTAGTAAAAACAGCCCCTGCACTTC
HDT1 ATGGAAGTACTAATTGCAAAGCAA TCATTGCCAGGTAGACGCCAGAGC
HDT2 ATGACTTTCAGCGGCGAAGGAAGGG TCATTGCCAGGTAGACGCCAGAGC
HDT3 ATGGGAATGGAACGCATGGGAACTTG TCATTGCCAGGTAGACGCCAGAGC
HDT4 ATGACAGGCGATAACGGAGTTGTTG TCAGCTTATGCTGGCCCTACTGGATC
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Gene cloning and bioinformatics analysis

The cDNA sequences of HMTs and HDTs were obtained from the L. japonica transcriptome database [28]. The open reading frames (ORFs) and deduced amino acid sequences of HMTs and HDTs were analyzed using ORF Finder (https://www.ncbi.nlm.nih.gov/gorf/gorf.html). The structural and functional domains were predicted by NCBI’s conserved domain database (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi). The neighbor-joining method was used to construct phylogenetic trees of HMTs and HDTs using MEGA 6.06 software. The phylogenetic tree was constructed based on the amino acid sequences of HMTs and HDTs from L. japonica and other species (Table S1).

Total histone extraction and concentration determination

Total core histone proteins (H2A, H2B, H3, and H4) of L. japonica were extracted using EpiQuik™ Total Histone Extraction Kit (Epigentek, USA), according to the kit manufacturer’s instructions. The total core histones were extracted by treatment with pre-lysis, lysis, and balance buffers. Post-translational modifications were kept intact and did not affect histone modification status or levels. The total histone concentration was determined using Modified Bradford Protein Assay Kit (Sangon Biotech, Shanghai, China), and histone concentration was calculated from the histone concentration curve.

Analysis of H3K4 and H3K9 methylation levels

Bioinformatics analysis showed that HMTs and HDTs were involved in H3K4 and H3K9 methylation. Therefore, methylation levels of histones H3K4 and H3K9 were measured using the EpiQuik™ Global Pan-Methyl Histone H3K4 Quantification Kit (Fluorometric, Epigentek, USA) and the EpiQuik™ Global Pan-Methyl Histone H3K9 Quantification Kit (Fluorometric, Epigentek, USA), respectively, following the kit manufacturer’s instructions.

Subsequently, H3K4 and H3K9 methylation curves were established. The standard curves included: (1) protein concentration vs. absorbance value, Y = 0.02X + 0.3124 (r = 0.9895); (2) H3K4 methylation level vs. fluorescence value, Y = 87.532X + 0.1914 (r = 0.9903); and (3) H3K9 methylation level vs. fluorescence value, Y = 93.662X + 0.1691 (r = 0.9882).

Based on the fluorescence value of 530EX/590EM nm, H3K4 and H3K9 methylation levels were calculated from the H3K4 and H3K9 methylation curves. The concrete formula used for calculation of relative methylation level was: Amount of protein (ng/mg) = RFU (sample − blank) × 1000 / protein (μg) × slope.

Gene expression analysis

Transcriptional levels of HMTs, HDTs, and key genes involved in active compound biosynthesis were analyzed using quantitative real-time polymerase chain reaction (qRT-PCR). The specificity of amplification was assessed using melting curve analysis, and the relative expression of genes was determined using the comparative Ct method. qRT-PCR analysis was performed using LightCycler®480 and SYBR® Premix Ex Taq™ (TaKaRa Biotechnology Co., Dalian, China), according to the manufacturer’s protocol. The house-keeping gene Lj18S was used as an internal control for each reaction. Primers for HMTs, HDTs, key genes involved in active compound biosynthesis, and housekeeping gene were designed using Primer Premier 5.0 (Table S2). The relative gene expression level compared to that of control was calculated using the 2−ΔCt method.

UPLC–MS/MS analysis and quantification

Chromatographic analysis was performed using Acquity UPLC I-Class system (Waters, Milford, MA, USA). The column used was Acquity UPLC BEH C18 column (2.1 mm × 100 mm, 1.8 μm), and the column temperature was maintained at 40 °C. The binary gradient consisted of solvent system A (formic acid/water, 0.1:99.9, v/v) and solvent system B (formic acid/acetonitrile, 0.1:99.9, v/v). The chromatographic conditions were as follows: 0 min, 5% B; 5.5 min, 30% B. The injection volume was 1.0 μL, and the flow rate was 0.50 mL·min−1.

Tandem mass spectrometry (MS/MS) was performed using QTRAP 6500 system (AB SCIEX, Los Angeles, CA, USA) equipped with an electrostatic ionization (ESI) source (AB SCIEX). MS analysis was carried out in negative ionization mode by monitoring the protonated molecular ions under the following operating conditions: ion source voltage, − 4500 V; and turbo spray temperature (TEM), 550 °C. The MS parameters for chlorogenic acids, flavonoids, and iridoids were manually optimized. Quantification was performed using multiple reaction monitoring (MRM) mode for the above transitions. Data were acquired using Analyst Software 1.6.2 (AB SCIEX, Los Angeles, CA, USA) and analyzed using MultiQuant Software 3.0 (AB SCIEX, Los Angeles, CA, USA).

To investigate the active compounds of L. japonica treated with 5-azaC, ultra performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) was performed. The optimized mass spectrum conditions of UPLC–MS/MS for chlorogenic acids, flavonoids, and iridoids are given in Table S3. Linear relation tests revealed that these compounds had a good linear relationship (r > 0.995) in their linearity ranges (Table S4).

Results

Sequence analyses of HMTs and HDTs

A preliminary BLASTx search was performed using the transcriptome sequencing dataset of L. japonica. The sequences of four HMTs and four HDTs of L. japonica were screened out and submitted to GenBank (accession numbers: KX812448–KX812455). Details of the HMTs and HDTs are shown in Table 2.

Table 2.

Sequences of four HMTs and four HDTs of L. japonica

Name Accession number Sequence length (bp) Amino acids number (aa) pI MW/KDa Conserved domains
HMT1 KX812448 1485 494 6.18 55.84 SET
HMT2 KX812449 1140 379 5.31 42.75 AdoMet-MTases
HMT3 KX812450 1614 537 5.43 59.89 AdoMet-MTases, PRMT5
HMT4 KX812451 2187 728 8.55 80.05 SAD-SRA, Pre-SET, SET
HDT1 KX812452 3567 1188 6.82 136.34 JmjC
HDT2 KX812453 3018 1005 5.57 113.98 JmjC
HDT3 KX812454 2226 741 7.88 84.93 JmjC, JmjN
HDT4 KX812455 1578 525 5.35 57.78 FAD/NAD, Amino oxidase, SWIRM
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The HMT1 gene was 1485 bp in length, and it encoded 494 amino acids. The isoelectric point (pI) value and the molecular weight (MW) of the HMT1 protein were calculated using the Compute pI/Mw tool of the ExPASy online server and found to be 6.18 and 55.84 kDa, respectively, and it contained one functional conserved domain i.e., the SET domain. The HMT4 gene was 2187 bp in length, and it encoded 728 amino acids. The pI value and MW of the HMT4 protein were 8.55 and 80.05 kDa, respectively, and it contained three conserved domains, including SAD-SRA, Pre-SET, and SET.

The HDT2 gene was 3018 bp in length, and it encoded 1005 amino acids. The pI value and MW of the HDT2 protein were 5.57 and 113.98 kDa, respectively, and it contained the JmjC conserved domain.

In order to better understand the functional details of HMTs and HDTs, phylogenetic trees were constructed with the amino acid sequences of histone methyltransferases and demethylases of other species using the neighbor-joining method. The bootstrap condition was 1000 times. Description of different types of histone methyltransferases and demethylases used in construction of phylogenetic trees are listed in Table S1.

Based on sequence similarity, the identified HMTs were clustered into two subgroups. It was observed that HMT1 and HMT4 of L. japonica and histone lysine methyltransferases were clustered in one clade (Fig. 1a). Therefore, HMT1 and HMT4 of L. japonica containing the SET domain were considered to be histone lysine transferases, combined with phylogenetic analysis, suggested that HMT1 and HMT4 belong to histone H3K9 methyltransferases group. Moreover, it was observed that HMT4 of L. japonica was located close to histone methyltransferase H3 lysine-9 specific SUVH1 of A. thaliana, and SUVH1 was found to maintain H3K4 methylation levels. Similarly, HDT2 were closely related to lysine-specific demethylase JMJ25 (Fig. 1b), which removes H3K4 methyl markings in gene bodies.

Fig. 1.

Fig. 1

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Phylogenetic tree of HMTs and HDTs. a Phylogenetic tree of HMTs; and b phylogenetic tree of HDTs. The bootstrap was 1000

Dynamic changes in the levels of HMTs/HDTs and H3K4/H3K9 methylation levels in 5-azaC-treated samples

Expression levels of HMTs and HDTs were analyzed using qRT-PCR, and it was observed that 5-azaC treatment significantly decreased the expression levels of HMT1 and HMT4 (p < 0.05) and increased the expression levels of HMT2, HMT3, and HDT2 (p < 0.05) (Fig. 2).

Fig. 2.

Fig. 2

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Relative gene expression levels of HMTs and HDTs in L. japonica. a Gene expression level of HMTs; and b gene expression level of HDTs. Control group, untreated leaves; 5-azaC group, leaves treated with 5-azaC; *compared with the control group, p < 0.05

Our results (Fig. 3) suggest that histone H3K9 methylation level increased after 5-azaC treatment because HMT1, HMT4, and HDT2 were all related to histone H3K9. Analysis of H3K4 and H3K9 methylation levels further showed that 5-azaC treatment significantly decreased H3K4me3 methylation level and significantly increased H3K9me1, H3K9me2, and H3K9me3 methylation levels.

Fig. 3.

Fig. 3

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Histone methylation levels in untreated and 5-azaC-treated leaves of L. japonica. a H3K4 methylation levels; and b H3K9 methylation levels. Control group, untreated leaves; 5-azaC group, leaves treated with 5-azaC; *compared with the control group, p < 0.05, **compared with the control group, p < 0.01

Chlorogenic acid, flavonoid, and iridoid content in 5-azaC-treated samples

In L. japonica treated with 80 μM 5-azaC for 48 h, it was observed that the levels of chlorogenic acids (chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, isochlorogenic acid A, and isochlorogenic acid C) and iridoids (loganic acid, loganin, secoxyloganin, and 7-epi-loganin) were significantly decreased (p < 0.05), whereas the level of flavonoids (luteolin and luteoloside) was significantly increased as compared to the control group (Table 3).

Table 3.

Chlorogenic acid, flavonoid, and iridoid content of L. japonica treated with 5-azaC

Category Analyte Control (μg/g) 5-azaC (μg/g)
Chlorogenic acids Chlorogenic acid 1903.44 ± 133.24 576.82 ± 51.91*
Neochlorogenic acid 74.67 ± 1.99 25.51 ± 2.52*
Cryptochlorogenic acid 14.43 ± 0.58 6.01 ± 0.20*
Isochlorogenic acid A 123.80 ± 8.58 31.48 ± 2.20*
Isochlorogenic acid C 13.53 ± 0.77 5.25 ± 0.37*
Flavonoids Luteolin 75.95 ± 5.32 427.01 ± 24.89**
Luteoloside 994.33 ± 33.81 1368.20 ± 46.52*
Iridoids Loganic acid 66.58 ± 4.29 32.46 ± 2.27*
Loganin 168.30 ± 16.32 72.26 ± 5.06*
7-epi-loganin 2536.57 ± 177.56 1037.17 ± 48.23*
Secoxyloganin 571.71 ± 40.02 196.32 ± 13.74*
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*Compared with the control group, p < 0.05, **Compared with the control group, p < 0.01

The results of qRT-PCR showed that the gene expression levels of HQT and 8HD1 decreased, while the expression levels of PAL, CHS, CHI, and FNSII increased significantly after 5-azaC treatment as compared to control. As the number of cycles in the logarithmic phase was greater than 35, it was considered that (3S)-linalool/(E)-nerolidol synthase (3LS), (E)-beta-ocimene/myrcene synthase (MOS), iridoid oxidase (IO), 7-deoxyloganic acid 7-hydroxylase (7D7H), ecologanin synthase (SS), and (E)-beta-ocimene/myrcene synthase (LAM) genes were not expressed or expressed at very low levels (Fig. 4). These results were in accordance with the content of bioactive compounds in L. japonica.

Fig. 4.

Fig. 4

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Expression levels of genes involved in active compound biosynthesis in L. japonica. Control group, untreated leaves; 5-azaC group, leaves treated with 5-azaC. PAL phenylalanine ammonia-lyase, C4H cinnamate 4-hydroxylase, 4CL 4-coumarate CoA ligase, HQT hydroxycinnamoyl-CoA quinate transferase, CHS chalcone synthase, CHI chalcone isomerase, FNSII flavone synthase II, 3LS (3S)-linalool/(E)-nerolidol synthase, IO iridoid oxidase, 7D7H 7-deoxyloganic acid 7-hydroxylase, 8HD 8-hydroxygeraniol dehydrogenase, LAM (E)-beta-ocimene/myrcene synthase. *p < 0.05; ***p < 0.001

Loganic acid and chlorogenic acid content showed significant positive correlation with H3K4me3 methylation level (p < 0.05); whereas, H3K9 methylation level showed significant positive correlation with luteolin content (p < 0.05) (Table S5).

Histone methylation levels and bioactive compounds in L. japonica from different producing areas

To further validate the relationship between H3K4/H3K9 methylation level and the content of bioactive compounds in L. japonica, we analyzed H3K4 and H3K9 methylation levels in L. japonica from different producing areas after plant introduction. The results indicated higher levels of H3K4me2 and H3K4me3 in buds obtained from Beijing than in buds obtained from Yunnan (Fig. 5a). Meanwhile, a significant difference (p < 0.05) in H3K9me3 level was also observed between buds from Yunnan and Beijing as well as between buds from Yunnan and Hebei (Fig. 5b).

Fig. 5.

Fig. 5

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Histone methylation levels and active compound content in L. japonica from different producing areas (Yunnan, Beijing, Hubei, and Hebei). a Methylation levels of H3K4; b methylation levels of H3K9; and c content of active compounds. *p < 0.05, **p < 0.01, ##p < 0.01, #p < 0.05

The relative content of chlorogenic acids was almost the same in the four producing areas. Luteolin level was significantly lower in buds from Hebei as compared to buds from Yunnan (p < 0.05). The content of loganic acid was also significantly different between buds from Yunnan and Hebei (p < 0.05) (Fig. 5c).

Discussion

Analysis of histone methylase in L. japonica

Epigenetic regulatory mechanisms involve DNA methylation, noncoding RNAs, and histone modification [37]. Results of protein domain analysis and BLAST suggest that HMT1 belongs to the SVUH9 subgroup of SU(VAR)3–9 family and HMT4 belongs to the SUVH1 subgroup of SU(VAR)3–9 family. SVUH9 from Arabidopsis thaliana silence transcribed genes through heterochromatin condensation [38], which is related to the methylation of H3K9. Therefore, we speculated that HMT1 is related to the methylation of histone H3K9. The SRA domain of the histone methyltransferase SUVH family directly binds to methylated DNA, and simultaneously demethylates histone H3K9 [39]. Studies have shown that SUVH1 is related to H3K9 methylation, but unlike SUVH9, it acts downstream of DNA methylation in the promoter region with anti-gene silencing effect, which is related to H3K4me3 [40]. Thus, we considered that HMT4 is both related to the methylation of histone H3K9me and H3K4me3.

Histone lysine demethylase can be classified into two major categories: lysine-specific demethylase 1 (LSD1) and Jmjc domain-containing histone demethylases (JHDM) [41]. HDT2 belonged to the JHDM group, and their sequences were similar to that of JMJ25/JMJ25-like H3K9 demethylase [42]. Lysine demethylases containing the JmjC domain could demethylate multiple sites including H3K4 and H3K9 [43]. Therefore, we concluded that HDT2 is related to the methylation of histone H3K9.

Effects of H3K4 and H3K9 methylation levels on active compound content and the expression levels of biosynthetic genes

Bioinformatic analysis of histone methyltransferase and demethylase genes from L. japonica transcriptome data showed that these genes are mainly associated with H3K4 and H3K9 methylation. Among them, H3K4me3 and H3K9me1/2/3 play a key role in histone methylation in L. japonica. The H3K4me3 region is mainly distributed in the euchromatin region, which is related to gene expression and promotes gene transcription [44]. Meanwhile, H3K9me is regarded as a hallmark of heterochromatin and gene silencing.

5-AzaC is a nucleotide analog that decreases DNA methyltransferase activity [45]. Recent studies have shown that 5-azaC influences histone H3K9me3 and H3K27me3 methylation levels [46]. Therefore, we attempted to illustrate the relationship among histone methylation, active compounds, and relative gene expression with the help of 5-azaC.

Histone methylation modification regulate the expression of key enzyme genes in the biosynthetic pathway of secondary metabolites in a variety of ways, which affects the accumulation of secondary metabolites to improve stress resistance in plants [47, 48]. The loss of ATX1 cause the levels of H3K4me3, NCED3 and ABA reduced in atx1 mutant [49, 50]. SDG8 and SDG25 contribute to plant immunity through histone methylation and regulating expression of plant immunity genes, accumulation of lipids, biosynthesis of carotenoids, and maintenance of cuticle integrity [51]. Combined with the results, we speculated that the significant changes in the content of active compounds and expression levels of related genes were due to variations in H3K9 and H3K4me3 levels. H3K9 methylation level negatively correlated with luteolin content, while H3K4me3 level positively correlated with chlorogenic acid and loganic acid content (Fig. 6). Therefore, histone methylation might influence secondary metabolite biosynthesis in L. japonica by transcriptionally regulating the expression of biosynthetic genes.

Fig. 6.

Fig. 6

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Schematic diagram of regulation of active compound biosynthesis by histone methylation. The red arrows indicate increased methylation levels, gene expression levels and content; and the green arrows indicate the opposite

Histone methylation levels and bioactive compounds in L. japonica from different producing areas

Chromatin remodeling affects a range of life processes, including flowering time, stress memory, and secondary metabolism [52]. It has been reported that regulation of histone methylation in plant defense and flowering is related to secondary metabolism [53]. However, it is unclear whether a similar link exists between active compound biosynthesis and histone methylation in medicinal plants from different regions. Fengqiu in Henan, Pingyi in Shandong, and Julu in Hebei are the three main L. japonica producing areas [54]. The phenotype of L. japonica and the methylation level of genes for key enzymes are closely related to the place of origin and are easily affected by biological and abiotic factors in the environment. DNA methylation and miRNAs play vital regulatory roles in phenotype of L. japonica [34, 55].

In order to exclude the impact of germplasm on histone methylation, similar varieties were selected from Beijing and introduced to three other geographical locations. The results showed that H3K4me2/3 methylation levels in L. japonica from Yunnan were significantly different from that in Beijing, while H3K9me3 methylation levels were significantly different between L. japonica from Yunnan and Hebei. Metabolomic analysis has revealed obvious differences in the chemical composition of L. japonica from different producing areas [56]. In this study, we found that luteolin content was different between L. japonica from Yunnan and Hebei. The main components of L. japonica including chlorogenic acid and loganic acid were not significantly different among L. japonica from the four producing areas. A comprehensive analysis of L. Japonica from four producing areas showed that the expression of genes involved in active compound biosynthetic pathway was also related to histone H3K4 and H3K9 methylation levels. Therefore, H3K4 methylation may regulate the chlorogenic acid and iridoid biosynthetic pathway in L. japonica. Thus, variation of histone methylation level in L. japonica indicates that the regulation of epigenetic modifications of active compounds is closely related to the environment.

Conclusion

In conclusion, our study is the first to demonstrate a potential regulatory network of H3K9/H3K4 methylation, transcription of secondary biosynthetic genes, content of secondary metabolites in L. japonica, and is also the first report revealing the regulatory mechanism of histone methylation in medicinal plants. Our findings on the role of histone H3K4 and H3K9 methylation in secondary metabolism of L. japonica open an interesting perspective for understanding the possible molecular mechanism of regulation of bioactive compound synthesis in functional plants. Moreover, our results demonstrate that the levels of histone H3K4me3 and H3K9me3 can be used as molecular markers for evaluating the quality of chlorogenic acids, flavonoids, and iridoids in L. japonica.

Electronic supplementary material

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11033_2020_5990_MOESM1_ESM.docx (1,006.3KB, docx)

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Acknowledgements

This research was financially supported by National Natural Science Foundation of China (NSFC) (81891013/81891010), the Fundamental Research Funds for the Central Public Welfare Research Institutes (ZZ10-008) and Key Project at Central Government Level for the Ability Establishment of Sustainable Use for Valuable Chinese Medicine Resources (2060302).

Abbreviations

PAL

Phenylalanine ammonia-lyase

C4H

Cinnamate 4-hydroxylase

4CL

4-Coumarate CoA ligase

HQT

Hydroxycinnamoyl-CoA quinate transferase

CHI

Chalcone isomerase

CHS

Chalcone synthase

IPP

Isopentenyl pyrophosphate

DMAPP

Dimethylallyl diphosphate triammonium salt

GPP

Geranyl pyrophosphate

GPPS

GPP synthase

GES

Geraniol synthase

G10H

Geraniol-10-hydroxylase

10HGO

10-Hydroxygeraniol

IS

Iridoid synthase

5-azaC

5-Azacytidine

ORFs

Open reading frames

HMT

Histone methyltransferase

HDT

Histone demethylase

H3K4me1/2/3

Monomethylation/dimethylation/trimethylation of histone H3 at lysine 4

H3K9me1/2/3

Monomethylation/dimethylation/trimethylation of histone H3 at lysine 9

3LS

(3S)-linalool/(E)-nerolidol synthase

IO

Iridoid oxidase

7H7D

7-Deoxyloganic acid 7-hydroxylase

8HD

8-Hydroxygeraniol dehydrogenase

LAM

(E)-beta-ocimene/myrcene synthase

MOS

(E)-beta-ocimene/myrcene synthase

SS

Ecologanin synthase

Author contributions

YY designed experiments; JY and SL performed the experiments; TL analyzed the data and wrote the original draft paper; YZ, JZ and YJ supervised the study; LH revised the manuscript. All authors have read and agreed to the published version of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Research involving human participants and/or animals

No human participants participated in this study.

Footnotes

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Tianrui Liu, Jian Yang, and Shuang Liu contributed equally to this work.

Contributor Information

Tianrui Liu, Email: raymond50@live.cn.

Jian Yang, Email: yangchem2012@163.com.

Shuang Liu, Email: scarlett0706@163.com.

Yuyang Zhao, Email: zhaoyuyang0618@163.com.

Junhui Zhou, Email: jh-zhou@outlook.com.

Yan Jin, Email: jy20047@163.com.

Luqi Huang, Email: huangluqi01@126.com.

Yuan Yuan, Email: y_yuan0732@163.com.

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