Changes in circulating stem cells and endothelial progenitor cells over a 12-month period after implantation of a continuous-flow left ventricular assist device

Abstract

Introduction

Changes in circulating CD34+CD45low stem cells (SC) and CD34+CD45low+KDR+ endothelial progenitor cells (EPC) may reflect pathological endothelial activation. Non-pulsatile/continuous-flow left ventricular assist devices (CF-LVAD) can enhance this process. The aim of this study was to analyse the impact of 12-month CF-LVAD treatment on SC and EPC.

Methods

We analysed changes in SC and EPC from the pre-implantation period up until 12 months after implantation over 3-month intervals in 14 patients. Data from 12 patients with heart failure (HF) and from 13 healthy volunteers were used as controls.

Results

Baseline EPC were significantly higher in CF-LVAD and HF patients than in healthy controls, substantially decreasing 3 months after CF-LVAD implantation and then returning to high baseline values at 12 months.

Conclusions

Changes in circulating SC and EPC may reflect pathological endothelial activation after CF-LVAD implantation.

Long-term use of ventricular assist devices (CF-LVAD) is an effective method for end-stage heart failure management [1, 2]. However, non-pulsatile flow patterns can activate the endothelium of the systemic vasculature, thus leading to clinical complications [3]. This process can be assessed by measuring circulating CD34+CD45low stem cells (SC) and CD34+CD45low+KDR+ endothelial progenitor cells (EPC) [4]. However, long-term studies of this phenomenon are scarce. Changes in SC and EPC levels have been observed under conditions associated with endothelial damage, including cardiovascular events. SC and EPC are also involved in general reparative processes [5–7]. Significantly, reduced levels of EPC have been found in advanced stages of heart failure [8].

In our previous work, we observed the biphasic response of SC and EPC 3 and 6 months after CF-LVAD implantation 9. The aim of the present study was to analyse the impact of CF-LVAD on SC and EC over a substantially longer period of 12 months.

Methods

Our single-centre prospective observational study was approved by the participating institute’s regulatory board and ethics committee before initiation. All patients provided their written informed consent to participate in the study.

Patients were implanted with a HeartMate II LVAD (St. Jude Medical, Pleasanton, CA), with clinical and laboratory data collected 24–48 h before and every 3 months ± 15 days up until 1 year after implantation. Samples were analysed immediately using flow cytometry to evaluate the expression of surface antigens, as reported previously [9]. Briefly, SC and EPC were determined by flow cytometry after staining with fluorescein isothiocyanate-conjugated anti-CD45, phycoerythrin-conjugated anti-CD34 and Alexa Fluor 647-conjugated anti- KDR markers. Analyses were performed using the Navios EX flow cytometer, with surface antigen expression determined based on at least 1 million cells of peripheral blood. SC and EPC were also measured in 13 healthy volunteers (5 women, mean age: 50.1 ±13.5 years) and in 12 patients with NYHA functional class III/IV heart failure (HF) (2 women, mean age: 52.2 ±7.2 years).

A generalised linear mixed model with random intercept and gamma regression was used to analyse longitudinal changes in EPC and SC. The results of these models are presented as the estimated marginal mean with a 95% confidence interval. Calculations were performed using SPSS 21 software (Armonk, NY, USA).

Results

Forty-five patients were originally enrolled in the study from March 2014 to March 2016. Over the 12-month period, 5 patients died and 13 were transplanted. A further 13 patients failed to complete follow-up either due to missed visit windows (n = 7) or technical difficulties precluding reliable measurements of SC and EPC (n = 6). There was no difference in baseline SC (p = 0.20) or EPC (p = 0.82) between patients (including deceased persons), with respect to the availability of complete or incomplete long-term follow-up data. Fourteen patients completed the 12-month follow-up (including 3 women, mean age: 54.2 ±12.6 years).

CF-LVAD and HF patients had significantly higher pre-implantation values of SC and EPC than healthy controls. No differences were found with respect to age, sex, aetiology of heart failure or prevalence of renal dysfunction between HF and CF-LVAD patients. As expected, the prevalence of smoking and diabetes was higher in both groups of patients than in controls (Table I).

Table I.

Population descriptive statistics

Parameter	Healthy controls (n = 13)	Type/effect (n = 12)
Age [years]	53.0 [41.0–58.0]	52.0 [47.0–57.5]
Sex (male), n (%)	8 (61.5%)	12 (100%)
Smoking, n (%)	1 (7.7%)	1 (8.3%)
Diabetes mellitus, n (%)	0 (0%)	6 (50%)*
Ischemic cardiomyopathy, n (%)	NA	5 (41.7%)
Creatinine [µmol/l]	NA	99 [86–125]
Urea [mmol/l]	NA	8.7 [6.8–11.2]
Haemoglobin [g/l]	NA	148.5 [136.0–161.0]
SC baseline [cells/ml]:	1889 (1005–3551)	3,061 (2,315–4,047)
3 months	NA	NA
6 months	NA	NA
9 months	NA	NA
12 months	NA	NA
EPC baseline [cells/ml]:	91 (60–136)	175 (115–268)*
3 months	NA	NA
6 months	NA	NA
9 months	NA	NA
12 months	NA	NA

Data are presented as estimated marginal mean (95% confidence interval), median [interquartile range], or frequency (percent). CF-LVAD – continuous flow left ventricular assist device, SC – CD34+CD45low stem cells, EPC – CD34+CD45low+KDR+ endothelial progenitor cells.

^*P < 0.05 vs. healthy controls.

After CF-LVAD implantation, SC had decreased at 3 months, increased at 9 months and moderately decreased at 12 months (Figure 1 A). The increase between 3 months and 9 months was statistically significant (p = 0.034). A moderately different pattern was observed for EPC (Figure 1 B), with a significant decrease at 3 months (p = 0.037) and an increase of borderline significance between 3 months and 9 months (p = 0.059). The above findings were independent of aetiology of heart failure (ischaemic vs. non-ischaemic), age and sex (p for interactions > 0.05). With regard to baseline values, we found no association between SC and EPC and major clinical events (death, stroke, pump thrombosis, non-surgical bleeding).

Figure 1.

Longitudinal changes of circulating CD34+CD45low stem cells (A) and CD34+CD45low+KDR+ endothelial progenitor cells (B)

Solid grey line – estimated marginal mean for healthy controls; solid black line – estimated marginal mean for advanced heart failure patients; dashed lines represent 95% confidence interval. SC – CD34+CD45low stem cells, EPC – CD34+CD45low+KDR+ endothelial progenitor cells.

Discussion

We observed substantial changes in circulating SC and EPC during the 12-month period following CF-LVAD implantation. SC and particularly EPC had decreased at 3 months before increasing at 6 months and 9 months. Both types of cells were moderately reduced at 12 months. We categorised changes over four periods to evaluate potential pathophysiological mechanisms. First, in relation to pre-implantation values, we found moderately higher numbers of SC and significantly higher numbers of EPC in pre-implant CF-LVAD and HF patients compared to healthy controls, potentially indicating an association between increased numbers of EPC and pathological endothelial activation caused by heart failure. Second, the reduction in both cell types 3 months after CF-LVAD implantation (values comparable to healthy controls) may not only have reflected transient endothelial deactivation (represented by EPC) but also overall improvement in tissue metabolism (represented by SC) due to dramatic improvements in haemodynamics. Third, the subsequent increase of both SC and EPC at 6 months and at 9 months may have reflected pathological endothelial reactivation caused by non-pulsatile flow, likely to have prevailed over haemodynamic stability. Fourth, the decrease of SC and EPC at 12 months may have reflected either endothelial adaptive processes or the exhaustion of endothelial regenerative capacity. It must be noted, however, that this final finding is the most speculative from a pathophysiological point of view.

The main limitations of our single-centre prospective study were the relatively low number of patients enrolled and the substantial dropout rate. Both factors impacted our ability to accurately evaluate the effects of our findings on clinical events, sex difference and other potential modifiers in this unique population. Another limitation of our study was the lack of analysis of other biomarkers associated with endothelial activation (von Willebrand factor) [10]. The main strength of our study is the employment of a long-term follow-up to assess parameters associated with vascular status in a rapidly expanding population of patients with heart failure treated with CF-LVAD. An association between these parameters and clinical events needs to be established by larger prospective studies.

In conclusion, circulating markers of vascular damage, particularly circulating CD34+CD45low stem cells and CD34+CD45low+KDR+ endothelial progenitor cells, can be a useful tool in assessing the effects of mechanical heart support on vasculature as well as on potential serious clinical events.