Table 1.
Assessment of tissue homogenates, pellet at 10,000g, sucrose fraction III, IV, V, VI and VII separated from human brain tissue by SG-UC method
| Tissue homogenates | Pellet at 10,000g | Fraction III | Fraction IV | Fraction V | Fraction VI | Fraction VII | |
|---|---|---|---|---|---|---|---|
| Mode size (nm)a | 113 | 139 | 172 | 148 | 138 | 139 | 119 |
| Particle numbera,b | 1.31E+12 | 3.21E+11 | 5.09E+10 | 1.42E+11 | 1.52E+11 | 6.77E+10 | 1.12E+10 |
| Total protein (μg)b,c | 3672 | 352.1 | 25.6 | 56.1 | 80.3 | 85.1 | 24.3 |
| Particles/proteins (μg) | 3.57E+08 | 9.12E+08 | 1.99E+09 | 2.53E+09 | 1.89E+09 | 7.96E+08 | 4.61E+08 |
a
Particle number and size of separated EVs were measured by Nanoparticle Tracking Analysis (NTA)
b
The starting material is 0.5g of human brain cortex grey matter
c
The total protein levels were measured by BCA assay.