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. 2020 Oct 30;10(26):12223–12240. doi: 10.7150/thno.50992

Figure 1.

Figure 1

The phosphorylation level of p38 is increased in both HCV-infected patient tissues and PHHs. (A-C) Upregulation of the phosphorylated p38 (P-p38) in HCV-infected human liver tissues. Liver cancer tissue sections from HCV-infected (HCV (+)) and HCV-uninfected (HCV (-)) patients were stained with anti-p38 and anti-P-p38 antibodies. IgG antibody was used as the primary antibody in the negative control. (A). Relative expression levels of phosphorylated p38 and total p38 in the liver tissues of HCV (-) and HCV (+) patients were quantitatively analyzed by Image-Pro Plus v6.0 software (B), and the statistics were shown (C). (D, E) Upregulation of P-p38 in PBMCs isolated from HCV-infected (HCV (+)) patients. PBMCs were isolated from representative HCV-infected patients and healthy donors. The phosphorylation of p38 (P-p38) and total p38 were detected by western blotting (D), and the ratio of phosphorylated p38 relative to total p38 was quantified by ImageJ software (E). (F, G) Upregulation of P-p38 in HCV-infected PHHs. PHHs were infected with JFH1 and the levels of total and phosphorylated p38 were then detected by western blotting (F). The fold change in phosphorylated p38 level relative to the total p38 level was quantified by ImageJ software (G).