Abstract
The P2Y12 receptor is an adenosine diphosphate responsive G protein-coupled receptor expressed on the surface of platelets and is the pharmacologic target of several anti-thrombotic agents. In this study, we use liver samples from mice with cirrhosis and hepatocellular carcinoma to show that P2Y12 is expressed by macrophages in the liver. Using in vitro methods, we show that inhibition of P2Y12 with ticagrelor enhances tumor cell phagocytosis by macrophages and induces an anti-tumoral phenotype. Treatment with ticagrelor also increases the expression of several actors of the endoplasmic reticulum (ER) stress pathways, suggesting activation of the unfolded protein response (UPR). Inhibiting the UPR with tauroursodeoxycholic acid (Tudca) diminishes the pro-phagocytotic effect of ticagrelor, thereby indicating that P2Y12 mediates macrophage function through activation of ER stress pathways. This could be relevant in the pathogenesis of chronic liver disease and cancer, as macrophages are considered key players in these inflammation-driven pathologies.
Keywords: inflammation, purinergic receptors, macrophages, liver disease, cancer
1. Introduction
Hepatocellular carcinoma (HCC) is a primary liver cancer that usually develops in a background of inflammation and chronic liver disease. Liver macrophages are phagocytotic immune cells that form the cornerstones in the inflammation-driven pathogenesis of liver cirrhosis and HCC [1]. Due to their location in the sinusoids and their ability to rapidly respond to a seemingly infinite variety of activating signals, they are the primary cells to react in case of liver injury [2]. Macrophages are heterogeneous by nature, as they play an active role in both inducing and resolving the inflammatory response [2,3]. The ability of macrophages to be reprogrammed or repolarized is an active area of research, especially in HCC, where macrophages have been reported to play a dual role depending on their polarization state [4]. Firstly, classically activated macrophages, often called M1 macrophages, exert a pro-inflammatory T helper type 1 (Th1) immune response and induce a tumoricidal activity [5]. Secondly, alternatively activated macrophages, often referred to as M2 macrophages or pro-tumoral macrophages, are associated with the anti-inflammatory T helper 2 (Th2) immune response and exert a pro-tumoral activity [6,7]. The balance between these anti-tumoral (M1) and pro-tumoral (M2) macrophages plays an important role in the development and progression of liver cancer. One of the factors involved in the switch between an anti- and pro-tumoral response of macrophages is the activation of the endoplasmic reticulum (ER) stress pathways. Endoplasmic reticulum stress is a cellular stress response that occurs when unfolded or misfolded proteins accumulate in the lumen of the ER, which leads to the induction of the unfolded protein response (UPR). This is sensed via three ER transmembrane proteins, namely, protein kinase R-like ER kinase (PERK), inositol-requiring enzyme 1 (IRE1α) and activating transcription factor 6 (ATF6), which become activated after dissociation from the ER chaperone binding immunoglobulin protein (BiP) [8]. Actors of the ER stress pathways are involved in numerous inflammatory processes, including the regulation of macrophage function and polarization [9,10,11]. It is therefore not surprising that ER stress pathways are crucial players in the pathogenesis of chronic liver diseases and HCC [11,12,13,14,15].
The P2Y12 receptor is an adenosine diphosphate (ADP) responsive G protein–coupled receptor expressed on the surface of platelets and is the pharmacologic target of several anti-thrombotic agents [16]. While the function of P2Y12 on platelets has been described extensively, less is known about the role of these receptors on other cell types [17]. However, extracellular ADP and related nucleotides promote a wide range of pathophysiological responses via activation of cell surface G-protein coupled purinergic receptors, while almost every cell type expresses P2 receptors. While ADP is predominantly known for its central role in the cellular energy metabolism, it is also widely distributed outside the cell where it influences diverse biological processes, including the inflammatory cell response. Platelets and tumor cells are known to release a great amount of adenosine triphosphate (ATP) and ADP in the tumor microenvironment, which could influence the inflammatory response through activation of P2Y12 [17]. Extracellular ATP has been shown to induce a strong pro-inflammatory response, whereas ADP generally serves as a negative feedback mechanism to promote an anti-inflammatory (M2) response and aid in the resolution of inflammation [18]. Even though not much is known about the role of P2Y12 in inflammatory cells, studies have reported that it is functionally present in microglial cells, the resident immune cells of the brain, where it plays a role in their activation [19,20]. In addition, other studies have shown a high expression of P2Y12 receptors in lymphocytes, monocytes and megakaryocytes [21], as well as cancer cells [22] and vascular smooth muscle cells [23]. However, the role of P2Y12 in these immune cells remains largely unknown. In this study, we show that P2Y12 is expressed by hepatic macrophages and provide evidence that this receptor plays a role in mediating macrophage polarization and function by inducing ER stress. Macrophages are considered key players in inflammatory pathologies, and altering their activation state and function could be relevant for patients with inflammation-driven cirrhosis and HCC.
2. Results
2.1. The P2Y12 Receptor Is Expressed in Mouse Models for Liver Cirrhosis and Hepatocellular Carcinoma
Liver tissue from healthy mice, mice with carbon tetrachloride (CCL4)-induced liver cirrhosis and mice with diethylnitrosamine (DEN)-induced HCC were immunohistochemically stained with P2Y12 antibodies (Figure 1A). This revealed a significant increase in the expression of P2Y12 in cirrhotic and HCC livers (Figure 1B), which was mainly distributed in the peri-vascular areas (Figure 1A). Co-staining of P2Y12 antibodies with F4/80 antibodies showed that P2Y12 expression colocalized with F4/80-positive macrophages (Figure 1C). This was further confirmed in vitro, as THP1-differentiated macrophages and the murine macrophage cell line RAW264.7 expressed the P2Y12 protein, shown by immunohistochemical staining (Figure 1D) and Western blot (Figure 1F). To assess whether P2Y12 levels would differ between pro-tumoral or anti-tumoral macrophages, P2Y12 protein levels were determined in interferon gamma and lipopolysaccharide-stimulated anti-tumoral (M1) macrophages and interleukin (IL)-13 and IL-4-stimulated pro-tumoral (M2) macrophages using Western blot and real-time qPCR (Figure 1F,G). No significant differences were seen in P2Y12 mRNA (Figure 1F) and protein expression (Figure 1G,H) between pro-tumoral (M2) and anti-tumoral (M1) macrophages.
Real-time qPCR was performed to determine the mRNA expression of P2ry12 in liver tissue from HCC, cirrhotic and healthy mice. In contrast to the results from the quantification of the immunohistochemical staining, we found that the mRNA expression was significantly decreased in the livers from mice with liver cirrhosis and liver cancer, compared to mRNA levels from healthy controls (Figure 1D). This discrepancy between protein levels and mRNA expression of P2ry12, has also been observed in other studies [24]. Thrombin stimulation has been shown to induce a modest and transient increase in mRNA expression of P2ry12 in vascular smooth muscle cells, while protein and cell surface expression of P2Y12 remain markedly increased over prolonged periods of time [24].
2.2. Expression of P2Y12 Is Located in the Stroma of Patients with Hepatocellular Carcinoma
To assess whether P2Y12 is expressed in patients with liver cancer, micrographs from liver biopsies were obtained from the Human Protein Atlas (Figure 2A) [25,26]. Histopathological evaluation of these liver biopsies stained with P2Y12 antibodies showed that the expression was mainly localized in the peritumoral areas and the fibrotic stroma (Figure 2B). Little to no staining was observed within malignant hepatocytes inside the tumor area (Figure 2C). There was no significant difference (p = 0.06) in survival between patients with high or low expression of P2Y12 (Figure 2D). No significant differences of P2Y12 expression were seen between the different tumor grades of HCC, (Figure 2E). Survival data and P2Y12 expression in different stages of HCC was obtained from publicly available data from the Human Protein Atlas [27].
2.3. Inhibition of the P2Y12 Receptor Decreases Viability of Macrophages
To study the effect of P2Y12 in vitro, we used the reversibly binding P2Y12 receptor antagonist ticagrelor. To determine if ticagrelor affected cell viability of macrophages and tumor cells, THP1-differentiated macrophages, RAW264.7 and HepG2 cells were exposed to 0–10 μM ticagrelor for 24 h. Inhibition of the P2Y12 receptor with ticagrelor decreased cell viability of both macrophage cell lines in a dose-dependent manner (Figure 3A). The THP1-differntiated macrophages were markedly more sensitive to ticagrelor treatment, as the concentrations that induced a 50% decrease in cell viability (IC-50 values) were 1.7 μM for THP1 macrophages and 4.4 μM for RAW264.7 cells. Since RAW264.7 cells are more differentiated and more heterogeneous by nature, resulting in the possible selection of separate subclones which could affect results [28], THP1-differentiated macrophages were used as a model system for our subsequent experiments [29]. To assess whether ticagrelor would have a different effect in pro-tumoral or anti-tumoral macrophages, the cell number of interferon gamma and lipopolysaccharide-stimulated anti-tumoral (M1) macrophages and IL-13 and IL-4-stimulated pro-tumoral (M2) macrophages was measured via a resazurin reduction assay. No significant differences were seen, thus suggesting that P2Y12 inhibition does not selectively affect survival of a specific macrophage phenotype (Figure 3B). Ticagrelor also decreased tumor cell viability, with a notable decrease in cell viability in concentrations higher than 2 μM (Figure 3C).
2.4. Ticagrelor Enhances Phagocytosis of Tumor Cells
To assess whether ticagrelor would affect macrophage function, we looked at phagocytosis of tumor cells by fluorescently labelling macrophages (CellTracker Red CMTPX) and tumor cells (CellTracker Green CMFDA) in a co-culture (Figure 4A,E). Macrophages that phagocytosed tumor cells were then identified by the occurrence of both green and red staining within the same cell. Treatment with ticagrelor lead to a significant increase in the number of macrophages that phagocytosed tumor cells (Figure 4B) and also increased the percentage of tumor cells that were removed by macrophages (Figure 4C). Ticagrelor significantly increased migration of macrophages (Figure 4D), as measured by quantifying displacement of the cells in 10-min time-lapse videos (Figure 4E and Supplementary Materials 1 and 2).
To see whether this difference in macrophage function was related to their polarization state, we stained macrophages with antibodies against human leukocyte antigen–DR-isotype (HLA-DR) (a pro-inflammatory M1 macrophage marker) and arginase-1 (a pro-tumoral M2 marker). Ticagrelor significantly increased expression of the pro-inflammatory macrophage marker HLA-DR and decreased expression of the pro-tumoral macrophage marker arginase-1 in THP1 macrophages stained with antibodies against HLA-DR and arginase-1, respectively (Figure 5A–C). The increased expression of HLA-DR was also confirmed using flow cytometry (Figure 5D,E). These results indicate that P2Y12 could play a role in mediating macrophage polarization and function.
2.5. Expression of ER Stress Genes Is Increased after P2Y12 Inhibition with Ticagrelor
One important mediator of the phenotypic shift between anti-tumoral and pro-tumoral macrophages is the activation of the UPR through the ER stress pathways. To assess whether actors of the ER stress pathways were affected by P2Y12 inhibition, we measured mRNA expression of markers from the three arms of the UPR, namely IRE1α, ATF6 and PERK. The most profound effect was through actors of the PERK branch, as CHOP and PERK mRNA levels were significantly increased after treatment with ticagrelor (Figure 6A,B). Activation of the IRE1α branch was also observed, since there was a significant increase of spliced XBP1 (XBP1-s) (Figure 6A,C), while levels of unspliced XBP1 (XBP1-u) remained unchanged (Figure 6A). The ER chaperone BiP—which can activate all three ER stress branches—showed a non-significant increase after ticagrelor treatment (Figure 6A,D) and treatment with ticagrelor did not affect the levels of ATF6 (Figure 6A). To evaluate if there was a dose-dependent effect on the expression of ER stress markers, THP1-differentiated macrophages were exposed to 0 µM, 0.5 µM and 1 µM ticagrelor. Notably, 1 µM ticagrelor lead to a 20-fold increase in the expression of CHOP (Figure 6B), which could have contributed to the toxicity of ticagrelor at higher concentrations, as CHOP is an important mediator in ER stress-induced apoptosis [30].
To assess whether inhibition of ER stress would affect phagocytosis in ticagrelor-treated macrophages, the ER stress inhibitor tauroursodeoxycholic acid (Tudca) was added to the phagocytosis assay before and during ticagrelor treatment (Figure 7A). Indeed, treatment with Tudca significantly decreased the number of macrophages phagocytosing tumor cells in the ticagrelor-treated macrophages (Figure 7B), as well as the number of tumor cells removed by macrophages (Figure 7C) and the overall movement of macrophages (Figure 7D). While a significant decrease in the number of HepG2’s was observed after 75 min of co-culture in the ticagrelor-treated group, treatment with Tudca seemed to restore this to similar levels as controls. These findings suggest that P2Y12 inhibition affects macrophage function in an ER stress dependent manner.
To assess whether the increased phagocytosis was due to a toxic effect of ticagrelor on the HepG2 cells (as macrophages would then rapidly clear the dead cells), we performed a live/dead cell assay in HepG2 cell co-cultured with THP1 macrophages. When measuring the percentage of live and dead HepG2 cells in the co-cultures, we observed no significant differences between control, ticagrelor, Tudca or the combination of ticagrelor and Tudca, suggesting that the observed effect on phagocytosis is not a result of a direct toxicity of any of the compounds used in this study (Figure 7F).
3. Discussion
Hepatocellular carcinoma is a clear example of an inflammation-related cancer, as more than 90% of HCC cases arise in the context of hepatic injury and inflammation. Chronic unresolved inflammation is associated with persistent hepatic injury and hepatocyte regeneration, leading to the development of fibrosis, cirrhosis, and eventually HCC. The perpetuated wound-healing response activated by parenchymal cell death and the resulting inflammatory cascade actively contributes to the hepatocarcinogenesis. Given the limited therapeutic efficacy in advanced HCC, prevention of HCC development could be an effective strategy for improving prognosis. Several pre-clinical and clinical studies have provided evidence that anti-platelet therapies, such as the P2Y12 antagonists clopidogrel and ticagrelor, slow down the progression of HCC by suppressing the intra-hepatic inflammatory response [31,32,33]. This has mainly been attributed to the effect on platelets, which drive tumorigenesis by acting on tumor cells and on different cells in the hepatic stroma [34]. In this study, we show an alternative route that can contribute to the therapeutic success of P2Y12 inhibitors. Using different animal models and cell lines, we show that P2Y12 is expressed by hepatic macrophages and provide evidence that this receptor plays a role in mediating macrophage polarization and function.
A similar role for P2Y12 has been described in microglial cells—the resident macrophages of the central nervous system—where the P2Y12 receptor is known to be involved in chemotaxis and mediates phagocytosis in case of nerve injury [19,20,35,36]. In our study, we show for the first time that hepatic macrophages also express this purinergic receptor and that it could be involved in mediating macrophage function. In vitro treatment of macrophage cell lines with a P2Y12 antagonist increased tumor cell phagocytosis and increased the expression of the anti-tumoral macrophage marker HLA-DR, while simultaneously reducing the expression of the pro-tumoral macrophage marker arginase-1. This is in line with the study of Moore et al., which shows that P2Y12 expression is increased in alternatively activated M2 microglia and P2Y12 antagonism decreases their anti-inflammatory function [20]. P2Y12 serves as a receptor for extracellular ADP, and adenosines are known to promote an anti-inflammatory phenotype as part of the resolution phase of an inflammatory response [18]. Other studies have also shown that the macrophage phenotype and function highly depend on ADP/ATP homeostasis, and higher ADP levels are found in M2 macrophages [37].
In our study, we observed an increased expression of several actors of the ER stress pathway after treatment with the P2Y12 antagonist ticagrelor, and blocking the ER stress pathway with Tudca, a well-known ER stress inhibitor, diminished the ticagrelor-induced pro-phagocytotic effect. Endoplasmic reticulum stress could therefore have played a major role in mediating macrophage function. Studies have shown that activation of the ER stress pathways is associated with an increased anti-tumoral (M1) phenotype, mainly through activation of PERK [9] and CHOP [38], which were also the ER stress actors that were highest in our mRNA analyses. However, the exact mechanism on how P2Y12 inhibition induces ER stress and how this contributes to the phenotypic switch in macrophages, warrants further research. A possible explanation is that ticagrelor activates the mitogen-activated protein kinase (MAPK) pathway. Studies on gastric epithelial cells have shown that clopidogrel—another P2Y12 antagonist—induces ER stress through activation of p38-MAPK [39]. In addition, the P2Y12 receptor has been shown to act on the MAPK pathway in microglial cells during nerve injury [40]. The MAPK pathway is a critical factor in the polarization of tumor-associated macrophages, as p38-MAPK and ERK1/2 activation push M2 macrophages towards the pro-inflammatory M1 phenotype [41]. However, studies have also shown that increased expression of UPR actors, such as BiP, IRE1, spliced XBP1 and PERK in macrophages, correlates with a pro-tumoral (M2) macrophage phenotype and function [42,43,44]. For instance, targeting PERK has been shown to directly promote M1-like anti-tumoral activity and clearance of tumor cells [44]. Important to note, however, is that UPR signaling is highly dependent on the stimuli that cause ER stress, as well as the cell type and the microenvironment that are affected. This could in part explain the contradicting findings on the role of ER stress in macrophages, as different environmental cues and possibly different macrophage origins could affect the threshold at which ER stress is induced, and which UPR arms will consequently be activated. Therefore, further research is needed to explore the exact role of UPR signaling in directing macrophage polarization, function and tumor-killing capacity.
The well-characterized P2Y12 antagonist ticagrelor was used to inhibit the P2Y12 receptor. Ticagrelor inhibits ADP signaling by inducing a conformational change in P2Y12 and thereby locking the receptor in an inactive state [45]. ADP can therefore still bind at its binding site, and the degree of receptor inhibition (and inhibition of ADP-induced signaling) is dependent on the concentration of ticagrelor [46]. It has been shown that ticagrelor has a 50% inhibitory concentration on P2Y12 of 0.074 ± 0.038 μM after 1 min and reaches equilibrium within 15 min [45]. It also decreases MeSADP receptor activation at a 50% inhibitory concentration of 0.059 ± 0.03 μM [45]. In our studies, we used 0.5 μM to 1 μM ticagrelor and longer incubation times than 1 min, indicating that the concentration and time used in our studies are likely sufficient to block the P2Y12 receptor. As we have not used any additional silencing techniques, due to the low viability after nucleofection of THP1 macrophages [47], it is important to note that our observations could be caused by an off-target effect. Ticagrelor is unique in having only one well-documented additional target of inhibition, namely a weak binding to the equilibrative nucleoside transporter 1 [48]. Other studies have shown that the inhibitory effect is weak, and functional relevance is limited to concentrations higher than 10 µM [49,50], due to ticagrelor´s high affinity to the P2Y12 receptor [45]. We can indeed not exclude that this off-target effect contributed to the results in our study, especially since the equilibrative nucleoside transporter 1 mediates the cellular uptake of nucleosides from the surrounding medium. However, it is important to note that we are using a dose that is far below the previously described threshold in order to lower even more the probability of an off-target effect.
To conclude, in this study we show that P2Y12 is expressed by hepatic macrophages in mice with liver cirrhosis and hepatocellular carcinoma. In vitro inhibition of P2Y12 with ticagrelor promoted an anti-tumoral phenotype and increased the expression of several actors of the ER stress pathways. More research is needed to elucidate the mechanism behind the P2Y12-dependent increase of ER stress and how this contributed to the phenotypic switch in macrophages.
4. Materials and Methods
4.1. Animal Models
Male C57Bl/6 (Crl) or sv129 mice were obtained from Scanbur (Denmark). Mice were housed in standard conditions with a normal dark–light cycle. Animals were acclimatized to their environment for 1 week and given ad libitum access to water and food throughout the experiments. For the cirrhotic mouse model, mice were injected twice per week with CCl4 (Sigma) (1:1 dissolved in olive oil; 1 mg/kg), and 5% alcohol was added to drinking water [51]. For the HCC mouse model, a DEN-induced model was used as previously described [52,53]. Five-week-old male Sv129 mice received intraperitoneal injections once per week with DEN (35 mg/kg bodyweight) diluted in saline. During experiments, mice were weighted twice per week, and their health was evaluated using the Uppsala University health monitoring template. This method was approved by the local ethical committee for animal experimentation (C95/14) and conforms to the Animal Research: Reporting of In Vivo Experiments guidelines developed by the National Centre for the Replacement, Refinement and Reduction of Animals in Research.
4.2. Cell Culture and Reagents
The HCC cell line HepG2 was cultured in Dulbecco modified Eagle medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco, ThermoFisher). THP1 monocytes were cultured as suspension cells in RPMI medium. Raw264.7 cells were grown in DMEM supplemented with 10% heat-inactivated FBS (Gibco). Misidentification of the cell lines was checked at the Register of Misidentified Cell Lines, and none of the chosen cell lines were on the list. Extracted DNA from all our cell lines are sent yearly to Eurofins Genomics (Ebersberg, Germany) for cell line authentication using DNA and short tandem repeat profiles. Authentication confirmed the correct identity of each cell line.
4.3. Differentiation of THP1 Cells
The monocyte cell line THP-1 was differentiated to a macrophage phenotype by treatment with phorbol-12-myristate-13-acetate and then further primed towards a pro-inflammatory activation state (M1) by treatment with interferon gamma and lipopolysaccharide or an anti-inflammatory state (M2) by treatment with IL13 and IL4, as previously described [47].
4.4. Proliferation
Cell proliferation was monitored via a resazurin reduction assay, as previously described [54]. A 1% solution of resazurin sodium salt (Sigma-Aldrich, Darmstadt, Germany) in phosphate-buffered saline (PBS) was filtered through a 0.22 μM filter. The filtered resazurin solution was added in 1/80 dilution to the cells and incubated for 24 h, after which fluorescent signal was measured with a 540/35 excitation filter and a 590/20 emission filter on a Fluostar Omega plate reader.
4.5. Phagocytosis Assay
THP1-differentiated macrophages were seeded on 8-well LabTek chambers, primed to a pro-inflammatory phenotype and incubated with CellTracker Red CMTPX dye (C34552, ThermoFisher, Uppsala, Sweden). HepG2 cells were incubated with CellTracker Green CMFDA dye (C7025, ThermoFisher, Uppsala, Sweden) and added to the THP1-differentiated macrophages at a concentration of 30,000 cells per well. Simultaneously, 0.5 µM ticagrelor and/or 100 µM Tudca was added to the co-culture, followed by 30 min incubation at 37 °C to allow HepG2 cells to reach the macrophage mono-layer. In the conditions where Tudca was added, macrophages had also been pre-incubated with 100 µM Tudca, prior to adding HepG2 cells. This concentration has previously been shown to effectively inhibit ER stress in different cell types [55,56]
Cell interactions were observed and imaged with an inverted confocal microscope (LSM 700, Zeiss, Stockholm, Sweden), using Plan-Apochromat 20× objectives and the Zen 2009 software (Zeiss, Stockholm, Sweden). Quantification of the phagocytosis rate was conducted by counting the number of cells that emitted both a red and green signal using ImageJ by Fiji. The following formulas were used to determine the percentage of macrophages phagocytosing tumor cells (Equation (1)) and the percentage of tumor cells phagocytosed by macrophages (Equation (2)).
Equation (1): Percentage of macrophages phagocytosing tumor cells
(1) |
Equation (2): Percentage of tumor cells phagocytosed by macrophages
(2) |
4.6. Immunohistochemistry
Tissue samples were fixed in 4% paraformaldehyde for 24 h and subsequently embedded in paraffin. Paraffin embedded tissue samples were cut at 5 μm and dried on SuperFrost plus glass slides overnight. Sections were de-paraffinized and rehydrated prior to staining. Antigen retrieval was performed by incubation at 37 °C with 0.05% trypsin and 0.1% CaCl2 solution in water. Blocking was carried out with TNB-blocking reagent (FP1020, Perkin-Elmer, Hägersten, Sweden) for 45 min and followed by an overnight incubation at 4 °C with primary antibodies against F4/80 (14-4801-82, ThermoFisher, Uppsala, Sweden) and P2Y12 (702516, ThermoFisher, Uppsala, Sweden). A 40-min incubation was used for the secondary antibody (Alexa 555 goat anti-rat (A-21434) and Alexa 488 donkey anti-rabbit (A-32790) from ThermoFisher, Uppsala, Sweden), and cell nuclei were stained with Hoechst for 8 min.
Cells were fixed for 10 min in 4% paraformaldehyde and subsequently washed with PBS. Prior to incubation with the primary antibodies against HLA-DR (PA5-22113, ThermoFisher, Uppsala, Sweden), arginase-1 (PA5-29645, ThermoFisher, Uppsala, Sweden) and P2Y12 (702516, ThermoFisher, Uppsala, Sweden) antigens were retrieved via heat-induced incubation with citrate buffer and blocking was done with TNB-blocking reagents. A 40-min incubation was used for the secondary antibody, Alexa 488 donkey anti-rabbit, (A-32790, ThermoFisher, Uppsala, Sweden) and cell nuclei were stained with Hoechst for 8 min.
Images were taken using an inverted confocal microscope (LSM 700, Zeiss, Stockholm, Sweden) using Plan-Apochromat 20× objectives and the Zen 2009 software (Zeiss, Stockholm, Sweden). The different channels of immunofluorescent images were merged using Fiji ImageJ software. Quantifications were performed blindly with ImageJ software by conversion to binary images for each channel and automated detection of staining on thresholded images using a macro. Movement of cells was measured using the TrackMate plugin in Fiji [57] on time-lapse videos that were obtained at 30 s intervals during a 10-min period. Cell movement is expressed as displacement over their entire trajectory during 20 frames.
4.7. SDS-PAGE and Western Blot
Protein lysates in lysis buffer were mixed with 2× Laemmli buffer and heated to 95 °C for 5 min before being loaded onto a Precast Mini-Protean® TGX™ gels (456-9034, Biorad, Solna, Sweden). After separation, proteins were transferred to an Immobilon®-Fl membrane (IPFL0010, Millipore, Solna, Sweden). The membrane was blocked using the Intercept® (Tris Buffered Saline) blocking buffer (927-60001, Li-Cor, Bad Homburg, Germany) diluted 1:4 in PBS, and sequentially incubated with P2Y12 antibodies and goat-anti-rabbit Alexa 680 (A21088, ThermoFisher, Uppsala, Sweden) PBS + 0.1% Tween®20 or blocking buffer with 0.1% Tween®20 and 0.01% SDS, respectively. Beta-actin antibodies were used as loading control (ab6276, Abcam, Cambridge, UK) and incubated with IRDye 800CW Goat anti-mouse (Li-Cor, Bad Homburg, Germany,). All incubations were carried out at room temperature for 1 h or overnight at 4 °C. The membranes were scanned using an Odyssey scanner (Li-Cor, Bad Homburg, Germany).
4.8. Flow Cytometry
THP1 cells were seeded into 6-well plates at 3 × 105 cells/well and differentiated to a pro-inflammatory (M1) phenotype as previously described [47]. Differentiated macrophages were then treated with 0.5 µM ticagrelor for 24 h, followed by flow cytometry to measure HLA-DR protein expression. Briefly, cells were harvested via a 15-min heat-incubation with accutase (00-4555-56, ThermoFisher, Uppsala, Sweden), pelleted, and washed with PBS. Cells were then incubated with APC anti-human HLA-DR antibody (clone L243, Biolegend, Taby, Sweden) and diluted 1:25 in cell staining buffer (420201, Biolegend, Taby, Sweden) for 45 min in the dark, at room temperature. Cells were subsequently washed three times with cell staining buffer, and fluorescence was measured using the BD Accuri C6 Plus flow cytometer. Results are shown as percentage of mean APC-HLA-DR fluorescence intensity of the HLA-DR+ control condition.
4.9. RT-qPCR
RNA was isolated from tissue or cell culture using the EZNA® RNA isolation Kit II (R6934-02, VWR, Spånga, Sweden). RNA concentration and purity were assessed using Nanodrop, after which 500 ng of mRNA was reverse transcribed with an iScript select cDNA synthesis kit (1708897, Bio-Rad, Solna, Sweden). Amplifications were conducted using the following primers (Table 1). mRNA expression was normalized to 18S, GAPDH and/or TBP1. Fold change was calculated via the delta–delta CT method, by using the average CT value of 3 technical replicates.
Table 1.
Target (Species) | Forward | Reverse |
---|---|---|
P2ry12 (mouse) | ACGAGTTTGTGAAGGCACGA | AGACTCACGACTTTCTTCTTTGGA |
P2ry12 (human) | TTTGCCCGAATTCCTTACAC | ATTGGGGCACTTCAGCATAC |
18S (human) | AGTCCCTGCCCTTTGTACACA | GATCCGAGGGCCTCACTAAAC |
CHOP (human) | CATCACCACACCTGAAAGCA | TCAGCTGCCATCTCTGCA |
XBP1-spliced (human) | AGACAGCGCTTGGGGATGGAT | CCTGCACCTGCTGCGGACTC |
BIP (human) | GAAAGAAGGTTACCCATGCAGT | CAGGCCATAAGCAATAGCAGC |
GAPDH (human) | GGAGCGAGATCCCTCCAAAAT | GGCTGTTGTCATACTTCTCATGG |
GAPDH (mouse) | AATGAAGGGGTCGTTGATG | GGTGAAGGTCGGTGTGAAC |
TBP1 (human) | AGTGACCCAGCATCACTGTTT | GGCAAACCAGAAACCCTTGC |
ATF4 (human) | TCAGACAGTACCAACGCTTATGC | GTTGTACCACAGTAGGCTGAGA |
ATF6 (human) | GTTGTACCACAGTAGGCTGAGA | TGGCCAAGCACTTCAAACCT |
IRE1 (human) | TAGTCAGTTCTGCGTCCGCT | TTCCAAAAATCCCGAGGCCG |
PERK (human) | ACGATGAGACAGAGTTGCGA | GCGCGTAAACAAGTTGCCT |
4.10. Human Protein Atlas
Micrographs from biopsies from HCC patients stained with antibodies against P2Y12 were obtained through the Human Protein Atlas and are available through http://www.proteinatlas.org [25,26]. Patient survival was correlated to expression levels of P2Y12, using publicly available data from the Human Protein Atlas [25,27]. Based on the fragments per kilobase of exon per million reads (FPKM) value of each gene, patients were classified into two expression groups, and the correlation between expression level and patient survival was examined using GraphPad Prism 8. Based on the data from the Human Protein Atlas, a cut-off of 0.03 FPKM was used to determine high or low expression. This value corresponded to the best expression cut-off level, meaning the FPKM value that would yield maximum difference in regard to survival between the two groups at the lowest log-rank p-value. Expression of P2Y12 in different tumor stages of HCC was derived from the Human Protein Atlas [25,27] using RNA sequence data from the Cancer Genome Atlas [58].
4.11. Live/Dead Cell Double Staining Kit
The percentage of live and dead HepG2 cells in co-cultures was measured by using a Live/Dead Cell Double Staining Kit (04511-1KT-F, Sigma-Aldrich, Darmstadt, Germany) following the manufacturer´s guidelines. Briefly, HepG2 cells were incubated with calcein-AM and propidium iodide solutions. Stained HepG2 cells were washed and then added to the THP1-differentiated macrophages. Percentages of live and dead cells were monitored using an inverted confocal microscope (LSM 700, Zeiss, Stockholm, Sweden) using Plan-Apochromat 20× objectives and the Zen 2009 software (Zeiss, Stockholm, Sweden). As living cells emit a strong green fluorescence (λex 490 nm, λem 515 nm) and dead cells emit red fluorescence (λex 535 nm, λem 617 nm) after excitation with a 488-laser, the number of fluorescently green and fluorescently red cells was simultaneously quantified to measure the percentage of live and dead cells amongst the HepG2 population.
4.12. Statistics
Data are presented as mean ± standard error of the mean. Statistical significance was determined using an unpaired, two-tailed Student’s T-test, and p-values < 0.05 were considered statistically significant. In vitro experiments were conducted in at least 3 biological replicates, which we define as parallel measurements of biologically distinct samples. We define technical replicates as loading of the same sample multiple times on the final assay. The in vivo experiments were performed on at least 5 independent animals.
Acknowledgments
The authors would like to thank Dick Wågsäter for donating the THP1 cells, visiting students Linus Edlund, Celine Notredame and Krister Gustafsson for their technical assistance during experiments, and Paul O´Callaghan for his valuable input on our project.
Abbreviations
ADP | Adenosine diphosphate |
ATF6 | Activating transcription factor 6 |
ATP | Adenosine triphosphate |
BIP | Binding immunoglobulin protein |
CCL4 | Carbon tetrachloride |
DEN | Diethylnitrosamine |
DMEM | Dulbecco modified eagle medium |
FPKM | Fragments Per Kilobase of exon per Million |
HCC | Hepatocellular carcinoma |
IRE1α | Inositol-requiring enzyme alpha |
IL | Interleukin |
MAPK | Mitogen-activated protein kinase |
PBS | Phosphate buffered saline |
PERK | Protein kinase R-like endoplasmic reticulum kinase |
Tudca | Tauroursodeoxycholic acid |
UPR | Unfolded protein response |
Supplementary Materials
The following are available online at https://www.mdpi.com/1422-0067/21/21/8177/s1.
Author Contributions
Conceptualization, F.H. and P.G.; methodology, F.H., N.P. and M.K.; software, F.H., N.P., and M.K.; validation, N.P. and M.K., formal analysis, F.H. and N.P.; investigation, N.P. and M.K.; resources, F.H. and P.G.; data curation, N.P. and M.K.; writing—original draft preparation, F.H.; writing—review and editing, F.H., N.P., M.K. and P.G. visualization, F.H.; supervision, F.H. and P.G.; project administration, F.H., funding acquisition, F.H. and P.G. All authors have read and agreed to the published version of the manuscript.
Funding
This research was funded by the Swedish Cancer Foundation (Cancerfonden, CAN2017/518 and CAN2013/1273), the Swedish society for medical research (SSMF, S17-0092), the O.E. och Edla Johanssons stiftelse and Olga Jönssons stiftelse.
Conflicts of Interest
The authors declare no conflict of interest.
Footnotes
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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