FIG 1.

Heterologous overexpression of the cysteine-rich proteins CdeA, CdeC, and CdeM in soluble (S) and insoluble (I) fractions of E. coli lysates. Recombinant proteins were expressed in E. coli BL21(DE3) pRIL carrying plasmids pARR22, pARR10, and pARR21, respectively, and induced with 0.5 mM IPTG for 16 h at 37°C. The cells were disrupted in soluble or insoluble lysis buffer and electrophoresed in 15% SDS-PAGE gels; His-tagged immunoreactive proteins were detected by Western blotting as described in Materials and Methods. Each lane was loaded with 2 μg of protein lysate. Molecular mass (kDa) markers are indicated on the left.