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. 2020 Nov 13;6(11):e05468. doi: 10.1016/j.heliyon.2020.e05468

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© 2020 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Effects of different TDZ concentrations on in vitro shoot organogenesis in ‘Delite’ rabbiteye blueberry using WPM culture medium. Different views of explants and shoots under a stereomicroscope (a–f) and digital camera (g–j). (a) TDZ 0 μM, showing an oxidized leaf explant. (b) TDZ 0.5 μM with many small (black arrow), medium (gray arrow), and large (white arrow) shoots. (c) TDZ 1.0 μM, with small (black arrow), medium (gray arrow), and large (white arrow) shoots. (d) TDZ 1.5 μM, with small (black arrow), medium (gray arrow), and large (white arrow) shoots. (e) TDZ 2.0 μM, with small (black arrow), medium (gray arrow), and large (white arrow) shoots. (f) TDZ 2.5 μM, with small (black arrow), medium (gray arrow), and large (white arrow) shoots. (g–j) Development of the shoots at 20 weeks after the first evaluation in fresh culture medium with 2.5 μM zeatin. Details of in vitro rooting in (h) and (j). Abbreviations: Ex, explant; R, roots; TDZ, thidiazuron; WPM, woody plant medium.