Figure 2.

Relative gene expression of immune checkpoints, suppressive molecules, methyltransferases and demethylation enzymes in circulating CD33⁺HLA-DR^– cells and antigen-presenting cells of healthy donors

The mRNA expression levels for PD-L1, MMP9, galectin-9, TGF-β1, TIM-3, ARG1 and VISTA in the sorted CD33⁺HLA-DR⁺ and CD33⁺HLA-DR^– cells were determined by quantitative RT-PCR (a). The mRNA expression levels of DNMT3a, DNMT3b, TET1, TET2 and TET3 in CD33⁺HLA-DR⁺ and CD33⁺HLA-DR^– cells were determined by quantitative RT-PCR (b). The relative gene expression was normalized to β-actin. Results obtained from 10 donors per myeloid cell subset, and expressed as mean ± SEM. N.D; not detected.