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. 2020 May 18;15(12):1275–1288. doi: 10.1080/15592294.2020.1767373

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 3. — DNA methylation status of different genes in circulating CD33⁺HLA-DR^– cells and antigen-presenting cells

Representative plots show the DNA methylation status in PD-L1 (a), MMP9 (b), galectin-9 (denoted as Gal-9) (c), TGF-β1 (d), TIM-3 (e), ARG1 (f) and VISTA (g) in the sorted CD33⁺HLA-DR⁺ and CD33⁺HLA-DR^– cells as analysed by bisulphite sequencing of the gDNA. Methylation status of individual CpG motifs is shown in a colour-coded manner: white indicates unmethylation and grey indicates methylation. Bar plot shows the overall average methylation percentage of all genes investigated (h). Results obtained from 10 donors and expressed as mean ± SEM.