Video 2. Phase contrast time-lapse of the regulatory volume decrease of Lrrc8a knockout (KO) bone-marrow-derived macrophages (BMDMs).

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Lrrc8a KO BMDMs were incubated in an isotonic buffer (340 mOsm kg⁻¹) for 5 min before dilution to a hypotonic solution (117 mOsm kg⁻¹) for the duration of the recording. Images were captured every minute (n = 3, Scale = 20 µm).