Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Fmr1 | PubMed | 14265 | |
| Strain, strain background (Mus musculus, male) |
Fmr1 KO: FVB.129P2-Pde6b+
Tyrc-chFmr1tm1Cgr/J |
The Jackson Laboratory |
JAX: 004624 RRID:IMSR_JAX:004624 |
|
| Strain, strain background (Mus musculus, male) |
Ai9: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J |
The Jackson Laboratory |
JAX: 007909 RRID:IMSR_JAX:007909 |
|
| Strain, strain background (Mus musculus, male) |
conditional Fmr1 KO | Zhong et al., 2018 | N/A | |
| Antibody | Mouse monoclonal anti-FMRP |
DSHB | Cat#2F5-1, RRID:AB_10805421 |
(1:2.5) |
| Antibody | Rabbit polyclonal anti-c-FOS |
Millipore | Cat#ABE457, RRID:AB_2631318 |
(1:500) |
| Antibody | Mouse monoclonal anti-CaMKII |
Abcam | Cat# ab22609, RRID:AB_447192 |
(1:500) |
| Antibody | Goat polyclonal anti-rabbit IgG secondary antibody |
Thermo Fisher Scientific | Cat#A-11034, RRID:AB_2576217 |
(1:200) |
| Antibody | Goat polyclonal anti-mouse secondary antibody (Cy3-conjugated) |
Jackson ImmunoResearch Laboratories |
Cat#115-165-146, RRID:AB_2338690 |
(1:100) |
| Antibody | Goat polyclonal anti-mouse secondary antibody (Cy2-conjugated) |
Jackson ImmunoResearch Laboratories |
Cat#115-225-146, RRID:AB_2307343 |
(1:200) |
| Other | AAV-DJ-Syn-mCre-GFP | Hsu et al., 2019 | N/A | AAV virus expressing mCre-GFP |
| Other | AAV-DJ-Syn-Cre-GFP | Hsu et al., 2019 | N/A | AAV virus expressing Cre-GFP |
| Other | AAV8-Fos-ERT2- Cre-ERT2-PEST |
Gift from Karl Deisseroth lab Ye et al., 2016 |
N/A | AAV-Fos-TRAP |
| Other | AAV-DJ-EF1a-DIO- hM4D(Gi)-mCherry |
Stanford Virus Core | Stock ID: AAV-129, Lot#5289 |
AAV virus expressing Cre-dependent hM4D(Gi)-mCherry |
| Other | AAV-DJ-EF1a-DIO-mCherry | Stanford Virus Core | Stock ID: AAV-14, Lot#5020 |
AAV virus expressing Cre-dependent mCherry |
| Chemical compound, drug |
4-Hydroxytamoxifen (4-OHT) |
Sigma-Aldrich | Cat#H6278 | 10 mg/ml |
| Chemical compound, drug |
Clozapine N-oxide (CNO) | Tocris | Cat#4936 | 5 mg/kg |
| Chemical compound, drug |
Corn oil | Sigma-Aldrich | Cat#C8267 | |
| Software, algorithm | Freezeview | Coulbourn Instruments | https://www.coulbourn.com/ | |
| Software, algorithm | FreezeFrame 4 | Coulbourn Instruments | RRID:SCR_014429
https://www.coulbourn.com/ product_p/act-100a.htm |
|
| Software, algorithm | Viewer III tracking system | Biobserve | RRID:SCR_014337
http://www.biobserve.com/ behavioralresearch/ products/viewer/ |
|
| Software, algorithm | MED-PC IV | Med Associates | RRID:SCR_014296
https://www.med-associates.com/ product/deluxe-shuttle-box- avoidance-chamber- package-for-mouse/ |
|
| Software, algorithm | Nikon Elements | Nikon | RRID:SCR_014329
https://www.microscope. healthcare.nikon.com/products/ software/nis-elements/nis- elements-advanced-research |
|
| Software, algorithm | Prism 6 | GraphPad | RRID:SCR_002798
https://www.graphpad.com/ scientific-software/prism/ |