Abstract
Purpose:
To assess whether hypermetabolically-defined regions of interest (ROIs) on 3,4-dihydroxy-6-[18F]-fluoro-L-phenylalanine (FDOPA) positron emission tomography (PET) could be used to evaluate physiological features and whether there are measurable differences between molecular subtypes and tumor grades.
Methods:
Sixty-eight treatment-naïve glioma patients who underwent FDOPA PET and magnetic resonance imaging (MRI) were retrospectively included. Fluid-attenuated inversion recovery hyperintense regions (FLAIRROI) were segmented. FDOPA hypermetabolic regions (FDOPAROI, tumor-to-striatum ratios > 1) within FLAIRROI were extracted. Normalized maximum standardized uptake value (nSUVmax), volume of each ROI, and median relative cerebral blood volume (rCBV) and apparent diffusion coefficient (ADC) within FLAIRROI or FDOPAROI were calculated. Imaging metrics were compared using Students t or Mann-Whitney U tests. Area under the curve (AUC) of receiver-operating characteristic curves were used to determine whether imaging metrics within FLAIRROI or FDOPAROI can discriminate different molecular statuses or grades.
Results:
Using either FLAIRROI or FDOPAROI, the nSUVmax and rCBV were significantly higher and the ADC was lower in isocitrate dehydrogenase [IDH] wild-type than mutant gliomas, and in higher-grade gliomas (HGGs) than lower-grade gliomas (LGGs). The FDOPAROI volume was significantly higher in 1p19q codeleted than non-codeleted gliomas, and in HGGs than LGGs. Although not significant, imaging metrics extracted by FDOPAROI discriminated molecular status and tumor grade more accurately than those extracted by FLAIRROI (AUC of IDH status, 0.87 vs. 0.82; 1p19q status, 0.78 vs. 0.73; grade, 0.87 vs. 0.76).
Conclusion:
FDOPA hypermetabolic ROI may extract useful imaging features of gliomas, which can illuminate biological differences between different molecular status or tumor grades.
Keywords: FDOPA PET, hypermetabolic ROI, glioma, MRI
Introduction
Recently, various advanced magnetic resonance imaging (MRI) sequences, including perfusion imaging, diffusion weighted imaging (DWI), spectroscopy, and functional MRI, can easily be acquired during a clinical examination to diagnose primary gliomas and evaluate their physiological features. Positron emission tomography (PET) using radiolabeled amino acids, including 3,4-dihydroxy-6-[18F]-fluoro-L-phenylalanine (FDOPA), O-(2-[18F] fluoroethyl)-L-tyrosine (FET), and [11C] methyl-L-methionine, is often employed in neuro-oncological practice to identify metabolically active glioma tissue. FDOPA and FET PET have improved distribution and efficiency owing to the relatively long half-lives of fluorinated tracers (110 minutes) compared to carbon tracers (20 minutes). The use of FDOPA has grown rapidly especially in the United States, while that of FET has grown in Western Europe. Physiological MRI and amino acid PET can provide complementary metabolic information of gliomas [1].
For evaluation of MRI metrics, regions of interest (ROIs) of contrast-enhanced areas on T1-weighted images or hyperintense areas on T2-weighted/fluid-attenuated inversion recovery (FLAIR) images, referred to as anatomical ROIs, are most widely used for extracting representative features of physiological images. These anatomical delineation methods are also implemented in Response Assessment in Neuro-Oncology criteria for assessing tumor response to therapy [2, 3]. For evaluating amino acid PET metrics, the maximum standardized uptake value (SUVmax) is most commonly calculated using anatomical ROIs or visually placed spherical ROIs. Biological tumor volume (BTV), corresponding to hypermetabolic areas, and values of time-to-peak on dynamic PET studies are also extracted [4]. These multiparametric MRI and PET features were then compared between different tumor subtypes or World Health Organization (WHO) grades, integrated in regression analyses for differentiating groups or predicting prognoses [5, 6], and used as biomarkers for treatment responses [7].
Standard methods of creating ROIs have some limitations. Since not all gliomas, especially lower-grade gliomas (LGGs), show contrast enhancement, contrast-enhanced ROIs are generally applied to only images with higher-grade gliomas (HGGs), thus neglecting hypermetabolic areas of LGGs. Treatment-related changes, such as pseudoprogression and pseudoresponse, also affect the segmentation of contrast-enhanced ROIs. ROIs of FLAIR hyperintense regions can cover the whole tumors; however, these ROIs may include edema or hypoactive regions within gliomas, which may lead to misunderstanding of the biological activities of gliomas. Spherical ROIs can be manually overlaid on hypermetabolic areas on PET images; however, they potentially have a risk of selection bias, which may reduce reproducibility, and generally include a limited small area. Hence, there is a demand for developing a novel method to create unbiased but critical ROIs to extract specific imaging features of gliomas.
We hypothesized that extracting imaging metrics within FDOPA hypermetabolic ROIs may reflect unbiased physiological features within metabolically active gliomas. This method, to the best of our knowledge, has never been used for extracting MRI features. Although the molecular subtype and grade of gliomas were generally determined by resection or biopsy, clarifying imaging features of each subtype or grade of gliomas within metabolically active regions may help monitor therapeutic-related changes and predict prognosis later on. The objective of the current study was to assess whether hypermetabolically-defined ROIs on FDOPA PET could be used to evaluate tumor metabolism and whether there were measurable differences between molecular subtypes and tumor grades using this approach.
Materials and methods
Patient Selection
Overall, 68 patients with treatment-naïve and histologically confirmed gliomas who underwent FDOPA PET and MRI scans at our institution between 2007 and 2019 were retrospectively included. All patients were diagnosed with WHO grade II, III, or IV diffuse gliomas by surgical resection or biopsy according to the 2007 or 2016 WHO classification of the central nervous system tumors [8, 9]. The MRI scans were performed within 2 months of the corresponding PET scans. Patients were classified by isocitrate dehydrogenase (IDH) mutation status and 1p19q codeletion status, detected by conventional techniques [10]. When available, O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation status and epidermal growth factor receptor (EGFR) amplification status were obtained. No patients underwent stereotactic biopsy prior to FDOPA PET or MRI. The study has been approved by the institutional review board, and all subjects signed an informed consent form. The same dataset was used in a previous study [11], which evaluated voxel-wise correlation between FDOPA and MRI.
FDOPA PET Image Acquisition
FDOPA PET images were acquired with a high-resolution full-ring PET/CT scanner (ECAT-HR; CTI/MIMVista; Siemens, Knoxville, TN, USA) after the subjects fasted for more than 4 hours. Using previously reported procedures, FDOPA was synthesized and injected intravenously [12, 13]. CT scans were performed prior to the PET scans for attenuation correction. Three-dimensional FDOPA emission data were acquired for a total of 30 minutes. The data were integrated between 10–30 minutes following the injection to obtain 20-minute static FDOPA images after reconstruction. FDOPA PET images were reconstructed using an ordered-subset expectation maximization iterative reconstruction algorithm, consisting of six iterations with eight subsets [14, 15]. Then a Gaussian filter with a full width at half maximum of 4 mm was applied, resulting in voxel sizes of 1.34 × 1.34 × 3 mm. SUV maps of FDOPA were calculated based on the radioactive activity divided by the decay-corrected injected dose per body mass [16]. The resulting SUV maps were normalized (nSUV) relative to the median value of the contralateral healthy striatum [10, 17].
Magnetic Resonance Image Acquisition
Anatomical MRI consisted at least of standard T1-weighted pre- and post-contrast images at 2D axial turbo spin echo with 3 mm slice thickness and no interslice gap or 3D inversion prepared gradient echo images with 1–1.5 mm isotropic voxel size. FLAIR images were acquired at 3 mm slice thickness with no interslice gap using a 1.5-T or 3-T clinical MRI scanner.
For dynamic susceptibility contrast (DSC) perfusion MRI, a total dose of 0.1 mmol/kg of Gd-DTPA or Gd-BTDO3A (Magnevist or Gadavist; Bayer Healthcare Pharmaceuticals, Wayne, NJ, USA) was administered with 0.025 mmol/kg for the preload dosage to mitigate T1-based leakage contamination. The remaining 0.075 mmol/kg was used for dynamic bolus administration as previously described [18]. A two-minute gap was placed between the preload dose and the initiation of the baseline DSC-MRI. The DSC-MRI was acquired with slice thickness = 5 mm with no interslice gap, number of baseline acquisitions before contrast agent injection = 10–25, and number of timepoints =120. First, dynamic time-series images were motion-corrected using FSL software (mcflirt; FMRIB, Oxford, UK; http://www.fmrib.ox.ac.uk/fsl/). Second, relative cerebral blood volume (rCBV) maps were calculated using a bidirectional contrast agent leakage-correction algorithm to model contrast flux into and out of the vasculature [19]. Finally, a normalized rCBV map was computed by dividing the rCBV map by the median rCBV value of the contralateral normal-appearing white matter.
DWI was performed prior to injection of contrast agent using a single-shot echo-planar imaging sequence in the axial plane for nine patients, and was acquired with slice thickness of 3 mm with no interslice gap. ADC maps were calculated from the acquired DWI with b = 1000 s/mm2 and b = 0 s/mm2 images, and expressed in units of 10−6 mm2/sec. Diffusion tensor imaging (DTI) data was collected for 54 patients for whom conventional DWI was not obtained, and mean diffusivity maps were used as estimates of ADC values after motion-correction using FSL software (eddy and dtifit). The parameter of the DTI consisted of 12–64 equidistant diffusion-sensitizing directions with b = 1000 s/mm2, along with a single b = 0 s/mm2 image with slice thickness of 2–3 mm with no interslice gap.
Postprocessing and ROI Analysis
All MRI and PET images were registered to the post-contrast T1-weighted images for each patient using a six-degree of freedom rigid transformation and a mutual information cost function using FSL software (flirt) or Freesurfer software (tkregister2; Massachusetts General Hospital, Harvard Medical School, MA, USA; https://surfer.nmr.mgh.harvard.edu). To register the ADC maps, we rigidly aligned b = 0 s/mm2 images acquired during the DWI or DTI sequence to the post-contrast T1-weighted images and applied the transform matrix to the ADC maps. A single ROI was segmented based on the region of hyperintensity on T2-weighted FLAIR as FLAIRROI by a board-certificated neuroradiologist (H.T. with 13 years of clinical experience) with Analysis of Functional Neuroimages software (AFNI; NIMH Scientific and Statistical Computing Core; Bethesda, MD, USA; https://afni.nimh.nih.gov) using a semi-automatic procedure as previously described [20, 21]. Maximum nSUV (nSUVmax) was quantified within FLAIRROI. Also, a FDOPA hypermetabolic area (voxels with tumor-to-striatum ratios > 1) within FLAIRROI was extracted as FDOPAROI, which is equivalent to BTV. This cut-off of one was determined according to the previous suggestion [17, 22]. The volumes of FLAIRROI and FDOPAROI were measured in milliliters. Median rCBV and median ADC within FLAIRROI or FDOPAROI were also calculated separately. Figure 1 illustrates an example of segmentations of FLAIRROI and FDOPAROI in a 74-year-old male glioblastoma patient.
Fig. 1.
An example of post-processing on a 74-year-old male patient with a WHO grade IV, IDH wild-type, MGMT-unmethylated, and EGFR amplification-negative glioblastoma. After registration of all images to post-contrast T1-weighted images, an ROI of FLAIR hyperintensity is segmented (FLAIRROI: red area), and applied to FDOPA PET images. FDOPA hypermetabolic region (FDOPAROI: blue area) higher than the striatum within FLAIRROI is extracted. The nSUVmax within FLAIRROI and volume of each ROI are calculated. FLAIRROI and FDOPAROI are applied to rCBV and ADC maps, and median rCBV and ADC within each ROI are calculated
Statistical Analyses
The Shapiro-Wilk test was used to test for normality. Student’s t-tests and Mann-Whitney U tests were performed for normally and non-normally distributed data, respectively.
The following comparisons were performed between different IDH status (IDH wild-type [IDHwt] vs. IDH mutant [IDHm]), 1p19q codeletion status (non-codeleted vs. codeleted for IDHm gliomas), MGMT methylation status (unmethylated vs. methylated), EGFR amplification status (negative vs. positive), and tumor grade (LGGs [grade II] vs. HGGs [grade III and IV]). For evaluating MRI and PET metrics, a total of seven imaging metrics including nSUVmax, FLAIRROI volume, FDOPAROI volume, median rCBV within FLAIRROI and FDOPAROI, and median ADC within FLAIRROI and FDOPAROI were compared between different glioma groups.
For evaluating the discriminatory potential, a multiple logistic regression model was used to differentiate molecular statuses and tumor grades. The variables extracted by FLAIRROI or FDOPAROI were separately integrated into the regression models. Imaging variables calculated by FLAIRROI included FLAIRROI volume, median rCBV within FLAIRROI, and median ADC within FLAIRROI. Imaging variable calculated by FDOPAROI included FDOPAROI volume, median rCBV within FDOPAROI, and median ADC within FDOPAROI. The nSUVmax was additionally integrated as a single variable or to the imaging variables extracted by FDOPAROI. Receiver-operating characteristic (ROC) curves were used to determine whether each method could discriminate molecular statuses and tumor grades. The area under the curve (AUC) of ROC curve and the sensitivity and specificity of discrimination were evaluated as measures of model performance. Comparison between the ROC curves calculated by FLAIRROI and those calculated by FDOPAROI was also performed.
Statistical analyses were performed using R software (version 3.5.2; http://www.r-project.org/) and GraphPad Prism (Version 8.3; GraphPad Software, La Jolla, CA). Statistical significance was defined as P < 0.05.
Results
Overall, 68 treatment-naïve glioma patients (n = 26 female) with a mean age of 51.7 (standard deviation, 14.9) years at the time of PET examination were included (TABLE 1 and Supplemental TABLE 1). Perfusion imaging and DWI for 61 and 63 patients with clinically useful quality were obtained. All patients were evaluated using FLAIRROI, while 53 of the 68 included patients were evaluated using FDOPAROI, because the nSUVmax of gliomas in 15 patients (IDHwt: n = 2 grade II, n = 3 grade III, n = 1 grade IV; IDHm non-codeleted: n = 4 grade II, n = 2 grade III; IDHm codeleted: n = 3 grade II) were less than the threshold of one.
Table 1.
Patient demographics and molecular information
No. of patients | 68 | ||||
Female | 26 (38%) | ||||
Age ± standard deviation (year) | 51.7 ± 14.9 | ||||
WHO grade | II 29 (43%) |
III 25 (37%) |
IV 14 (21%) |
||
IDH mutation and 1p19q codeletion status | Wild-type | 36 (52%) | 7 | 15 | 14 |
Mutant 1p19q non-codeleted | 16 (24%) | 10 | 6 | 0 | |
Mutant 1p19q codeleted | 16 (24%) | 12 | 4 | 0 | |
MGMT promoter methylation status | Unmethylated | 27 (40%) | 9 | 11 | 7 |
Methylated | 27 (40%) | 11 | 10 | 6 | |
Unknown | 14 (21%) | 9 | 4 | 1 | |
EGFR amplification status | Negative | 38 (56%) | 16 | 14 | 8 |
Positive | 11 (16%) | 1 | 6 | 4 | |
Unknown | 19 (28%) | 12 | 5 | 2 |
IDH, isocitrate dehydrogenase; MGMT, O6-methylguani DNA methyltransferase; EGFR, epidermal growth factor receptor.
Figure 2 demonstrates the results comparing IDHwt (n = 36) and IDHm gliomas (n = 32). The nSUVmax was significantly higher in IDHwt than in IDHm gliomas (P = 0.034). The rCBV within both FLAIRROI and FDOPAROI were significantly higher (P = 0.007 and < 0.001, respectively) and ADC within FLAIRROI and FDOPAROI were significantly lower (both Ps < 0.001) in IDHwt than in IDHm gliomas. The imaging metrics calculated by FDOPAROI showed better discrimination ability of IDH status (AUC, 0.87; sensitivity, 100%; specificity, 65%) compared with those calculated by FLAIRROI (AUC, 0.82; sensitivity, 89%; specificity, 66%). When nSUVmax was included, discrimination was further improved (AUC, 0.91; sensitivity, 90%; specificity, 87%).
Fig. 2.
Imaging metrics of FDOPA PET and MRI, and the ROC curve in IDHwt (n = 36) and IDHm (n = 32). a) The nSUVmax is significantly higher in IDHwt than IDHm gliomas. b) The volume of FLAIRROI and FDOPAROI do not significantly differ. c) The rCBV within FLAIRROI and FDOPAROI are significantly higher in IDHwt than in IDHm gliomas. d) The ADC within FLAIRROI and FDOPAROI are significantly lower in IDHwt than in IDHm gliomas. e) ROC curves show the best AUC to differentiate IDH status when using FDOPAROI and nSUVmax (AUC 0.91, sensitivity 84%, specificity 87%). *, **, and *** mean P < 0.05, < 0.01, and < 0.001, respectively
Figure 3 summarizes the results comparing IDHm 1p19q non-codeleted (n = 16) and IDHm 1p19q codeleted gliomas (n = 16). The FDOPAROI volume was significantly higher in codeleted than non-codeleted gliomas (P = 0.022). The rCBV within FLAIRROI was significantly higher in codeleted than non-codeleted gliomas (P = 0.044). The imaging metrics calculated by FDOPAROI (AUC, 0.78; sensitivity, 56%; specificity, 100%) showed better discrimination ability of 1p19q codeletion status compared with those calculated by FLAIRROI (AUC, 0.73; sensitivity, 64%; specificity, 81%). When nSUVmax was included, discrimination was further improved (AUC, 0.83; sensitivity, 67%; specificity, 100%).
Fig. 3.
Imaging metrics of FDOPA PET and MRI, and ROC curves in IDHm 1p19q codeleted (n = 16) and non-codeleted gliomas (n = 16). a) The nSUVmax, b) volume of FLAIRROI and FDOPAROI, c) rCBV, and d) ADC are shown for different 1p19q codeletion statuses. Only FDOPA volume and rCBV within FLAIRROI are significantly higher in 1p19q codel than non-codel gliomas. e) ROC curves show the best AUC to differentiate 1p19q codeletion status when using FDOPAROI with nSUVmax (AUC, 0.83; sensitivity, 67%; specificity, 90%). * means P < 0.05
The results of MGMT and EGFR status are described in Supplemental Figures 1 and 2.
Figure 4 summarizes comparisons between LGGs (n = 29 grade II) and HGGs (n = 39 grade III and IV). The nSUVmax and FDOPAROI volume were significantly higher in HGGs than LGGs (P < 0.001 and 0.003, respectively). The rCBV within both FLAIRROI and FDOPAROI were significantly higher (P = 0.049 and < 0.001, respectively) and ADC within FLAIRROI and FDOPAROI were significantly lower (P = 0.001 and < 0.001, respectively) in HGGs than LGGs. The imaging metrics calculated by FDOPAROI (AUC, 0.87; sensitivity, 76%; specificity, 88%) exhibited a better ability to discriminate tumor grade compared with those calculated by FLAIRROI (AUC, 0.76; sensitivity, 69%; specificity, 83%). When nSUVmax was included, discrimination was further improved (AUC, 0.89; sensitivity, 76%; specificity, 94%).
Fig. 4.
Imaging metrics of FDOPA PET and MRI, and ROC curves in LGGs (n = 29 grade II) and HGGs (n = 39 grade III and IV). a) The nSUVmax and b) the FDOPAROI volume are significantly higher in HGGs than in LGGs. c) The rCBV within FLAIRROI and FDOPAROI are significantly higher in HGGs than in LGGs. d) The ADC within FLAIRROI and FDOPAROI are significantly lower in HGGs than in LGGs. e) ROC curves show the best AUC to differentiate IDH status when using FDOPAROI with nSUVmax (AUC, 0.89; sensitivity, 76%; specificity, 94%). *, **, and *** mean P < 0.05, < 0.01, and < 0.001, respectively
No ROC curve pairs calculated by FLAIRROI and FDOPAROI showed significant differences in the differentiation of IDH status, 1p19q status, nor tumor grade. The prediction abilities of each group for independent imaging metrics are shown in Supplemental Figure 3. When comparing the ROC curves of independent imaging metrics, only rCBV within FDOPAROI had a significantly better discrimination ability for tumor grade than rCBV within FLAIRROI (AUC: 0.85 vs. 0.65; P = 0.030).
Discussion
In this study we found that imaging metrics calculated by FDOPAROI exhibited improved discrimination between different molecular statuses and tumor grades than those calculated by FLAIRROI. These findings suggested that imaging metrics within metabolically active regions may capture more useful features of gliomas than those within anatomical ROIs.
FDOPA hypermetabolic areas referred to as BTV were used as ROIs for calculating physiological MRI metrics in this study. The threshold of FDOPA hypermetabolism was defined as the median value of the basal ganglia, which has been empirically used for FDOPA examinations [17, 22]. PET-derived delineation of a tumor is useful for evaluating responses to molecular targeted therapy and chemotherapy [7, 23, 24], and for predicting prognosis after chemoradiotherapy [25], regardless of contrast enhancement. Prior to this study, BTV had never been used as a ROI for calculating other imaging metrics, such as those from physiologic MRI. We hypothesized that our method using hypermetabolic ROIs could focus on hypermetabolic areas with high reproducibility, and mitigate confounding factors such as necrosis, bleeding, and edema within tumors, resulting in more accurate characterization of biologically active areas of gliomas [26]. However, the limitation of hypermetabolic ROIs is that the nSUVmax must be higher than the threshold. In line with a previous study reporting that about 30% of newly diagnosed gliomas were PET-negative against the background of amino acid PET [27], we chose to exclude 15 of the 68 (22%) gliomas for the evaluations using FDOPAROI due to their hypometabolism. Meanwhile, hypermetabolic ROIs may be useful when gliomas are suspected following standard MRI, and thereafter revealed to be amino acid PET-positive. In such cases, our new technique may extract more useful features of gliomas than the widely used standard technique using FLAIRROI. The hypermetabolic ROI used in this study may also be applicable to other PET tracers; hence, validation in other tracers is desired.
Diffuse gliomas were previously classified into astrocytomas, oligodendrogliomas, and oligoastrocytomas, ranging from WHO grade II to grade IV according to their histologic features. In 2016, the WHO classification of Tumors of the Central Nervous System reclassified gliomas by integrating molecular status, such as IDH gene mutation and chromosomal 1p19q co-deletion, as well as histologic characteristics [9]. Thereafter, for the treatment of gliomas, main interests related to the management and prognostication reside in IDH mutation status. Patients with IDHm gliomas often exhibit relatively favorable outcomes, while IDHwt gliomas often lead to poor prognosis [28]. In this study, IDHwt gliomas showed higher nSUVmax, hypermetabolic volume, and rCBV and lower ADC than IDHm gliomas for both FLAIRROI and FDOPAROI. Although a previous study comparing LGGs with IDHwt and IDHm showed higher nSUVmax in IDHm gliomas [29], this study showed higher nSUVmax in IDHwt gliomas, which may be partly due to that all grade IV gliomas in this study were IDHwt. Glioma grading is important in prognostication and in making treatment decisions. Increased FDOPA uptake predicted not only higher tumor grade, but worse outcomes [5]. This study revealed higher nSUVmax, hypermetabolic volumes, and rCBV, and lower ADC in HGGs than LGGs for both FLAIRROI and FDOPAROI. These results between different tumor grades are consistent with those from the previous study [4]. When evaluating the diagnostic performance, imaging metrics extracted by FDOPAROI improved discrimination compared with those extracted by FLAIRROI, suggesting that imaging metrics extracted by hypermetabolic ROIs may illuminate biological differences between different subtypes or tumor grades.
This study was subject to some limitations. First, although hypermetabolic ROI used in this study may be useful to extract imaging features within the hypermetabolic region with high reproducibility, amino acid PET uptake values within the ROIs must be higher than the thresholds. Second, although this study showed better AUC to differentiate IDH status, 1p19q status, and tumor grade by using FDOPAROI rather than FLAIRROI, the comparison of ROC curves using different methods did not significantly differ. Further validation in a larger population is required. Third, the patient age and sex may affect the FDOPA uptake in the background of the brain structures [30]; however, this study did not adjust for these factors. Fourth, due to the retrospective nature, the acquisition parameters and scanners of MRI were not identical across patients, and perfusion imaging and DWI were not acquired for all patients. A prospective study is needed to fully elucidate the potential of our novel technique in management of gliomas. Lastly, this study did not use contrast-enhanced regions as ROIs nor for volumetry because only one third of the gliomas in this study showed contrast enhancement (IDHwt, 16/36; IDHm-non-codel, 4/16; IDHm-codel, 2/16; HGGs, 18/39; LGGs, 4/29). However, evaluation of the associations or differences between contrast-enhanced ROIs and FDOPA hypermetabolic ROIs may reveal new aspects of gliomas, especially for IDHwt gliomas and HGGs.
Conclusion
FDOPA hypermetabolic ROI may extract useful, unbiased imaging features of gliomas, which can be used to illuminate biological differences between different molecular status and tumor grades, and lead to the discovery of novel imaging biomarkers.
Supplementary Material
Acknowledgments
Funding
Grant from the Society of Nuclear Medicine and Molecular Imaging (SNMMI) (Tatekawa); American Cancer Society (ACS) Research Scholar Grant (RSG-15-003-01-CCE) (Ellingson); American Brain Tumor Association (ABTA) Research Collaborators Grant (ARC1700002) (Ellingson); National Brain Tumor Society (NBTS) Research Grant (Ellingson, Cloughesy); NIH/NCI UCLA Brain Tumor SPORE (1P50CA211015-01A1) (Ellingson, Lai, Cloughesy, Nghiemphu); NIH/NCI 1R21CA223757-01 (Ellingson)
Footnotes
Conflicts of interest
Ellingson—Advisory Board— Hoffman La-Roche; Siemens; Nativis; Medicenna; MedQIA; Bristol-Myers Squibb; Imaging Endpoints; Agios. Paid Consultant—Nativis; MedQIA; Siemens; Hoffman La-Roche; Imaging Endpoints; Medicenna; Agios. Grant Funding—Hoffman La-Roche; Siemens; Agios; Janssen. Ellingson also holds a patent on this technology (US Patent #15/577,664; International PCT/US2016/034886). Cloughesy—Advisory Board—Roche/ Genentech, Amgen, Tocagen, NewGen, LPath, Proximagen, Celgene, Vascular Biogenics Ltd, Insys, Agios, Cortice Bioscience, Pfizer, Human Longevity, BMS, Merck, Notable Lab, MedQIA.
Ethics approval
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Written informed consent was obtained from all individual participants to have their imaging, clinical, and molecular data included in our research database (IRB IRB#10-000655).
Publisher's Disclaimer: This Author Accepted Manuscript is a PDF file of an unedited peer-reviewed manuscript that has been accepted for publication but has not been copyedited or corrected. The official version of record that is published in the journal is kept up to date and so may therefore differ from this version.
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