Abstract
Protein AMPylation is a prevalent protein post-translational modification in human cells involved in endoplasmic reticulum stress regulation and neural development. In this article we describe the design, synthesis and application of a pronucleotide probe suitable for in situ fluorescence imaging and chemical protemics profiling of AMPylated proteins. Our probe utilizes straightforward strain-promoted azidealkyne click reaction for fluorescence labeling in living cells.
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Footnotes
Funding note
Open Access funding enabled and organized by Projekt DEAL.
Tobias Becker 2013–2017 Bachelorstudium Chemie und Biochemie an der LMU München. 2017–2019 Masterstudium Biochemie an der TU München. Seit 2019 Promotion an der LMU München in der Nachwuchsgruppe von Dr. Kielkowski.
Pavel Kielkowski 2007–2009 Masterstudium Chemie der Naturstoffe am Institute of Chemical Technology, Prag, Tschechien. 2009–2014 Promotion Organische Chemie am Institute of Organic Chemistry and Biochemistry AS CR (IOCB), Prag und an der Charles University in Prag. 2014–2019 Postdoktorand an der TU München in der Gruppe von Prof. Dr. S. A. Sieber. Seit 2019 Nachwuchsgruppenleiter an der LMU München.
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