Abstract
Silurus asotus is a widely distributed species, and mainly lives in the middle and lower layers of rivers, lakes, and reservoirs. In this study, we report the complete mitochondrial genome of S. asotus, which was 17,385 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region. Phylogenetic analyses indicated that S. asotus has a close relationship with S. soldatovi. This research work will provide some useful information on further molecular evolution studies of Siluridae species.
Keywords: Silurus asotus, Siluridae, mitochondrial genome, phylogenetic
Silurus asotus is a worldwide distributed fish species. It usually inhabits in the bottom of the water, which grass clumps and water flowing slowly. Silurus asotus is a carnivorous benthic fish that feeds on small fish, shellfish, and frogs. Although, already many studies about Siluridae fishes are available (Wang et al. 2015; Xia et al. 2018), it is necessary to make more studies of this important economic species. Here, we report the complete mitogenome of S. asotus and its phylogenetic relationship.
The specimens of S. asotus were collected in Mudong of Chongqing (106°50′E, 29°34′N) and preserved in 100% ethanol in the museum of Chongqing Normal University until DNA extraction. The complete mitogenome sequence was 17,385 bp (GenBank: MK895951) and consist of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region. Most genes were encoded on the H-strand, except ND6 and seven tRNA genes. The overall base composition of the mitogenome was 31.3% A, 26.2% T, 27% C, and 15.5% G. The total length of 13 PCGs was 11,408 bp. Similar to other vertebrates, all of the 13 PCGs in the mitogenome of S. asotus began with ATG, except COI gene with GTG. Incomplete stop codon is usual in vertebrate mitogenome and could be completed by posttranscriptional polyadenylation (Liu et al. 2013; Bai et al. 2018). There are seven genes (ND2, COII, COIII, ATP6, ND3, ND4, and Cyt b) that used two incomplete codons, T–– and TA–. Other five genes (ND1, COI, ATP8, ND4L, and ND5) used complete stop codon TAA, whereas ND6 used TAG.
To verify the phylogenetic position of S. asotus in family Siluridae, we utilized other nine Siluridae fishes and two outgroup species (Loricaria cataphracta, Plotosus japonicus). Maximum likelihood (ML) phylogenetic analysis was performed using MEGA 7 (Kumar et al. 2016) based on the concatenated 13 PCGs of these all species. Topological structure of the tree showed that S. asotus was clustered with S. soldatovi (BP = 100) (Figure 1). It is obvious that the phylogenetic position of S. asotus was closer to S. soldatovi.
Figure 1.
Maximum likelihood (ML) tree showing the phylogenetic position of Silurus asotus among Siluridae species based on a dataset of 13 PCG sequences. Only bootstrap support (BP) greater than 50% are shown above the lines.
Disclosure statement
No potential conflict of interest was reported by the authors.
References
- Bai J, Xu S, Nie Z, Wang Y, Zhu C, Wang Y, Min W, Cai Y, Zou J, Zhou X. 2018. The complete mitochondrial genome of Huananpotamon lichuanense (Decapoda: Brachyura) with phylogenetic implications for freshwater crabs. Gene. 646:217–226. [DOI] [PubMed] [Google Scholar]
- Kumar S, Stecher G, Tamura K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 33:1870–1874. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liu T, Jin X, Wang R, Xu T. 2013. Complete sequence of the mitochondrial genome of Odontamblyopus rubicundus (Perciformes: Gobiidae): genome characterization and phylogenetic analysis. J Genet. 92:423–432. [DOI] [PubMed] [Google Scholar]
- Wang QR, Xu C, Xu CR, Wang RJ. 2015. Complete mitochondrial genome of the Southern catfish (Silurus meridionalis Chen) and Chinese catfish (S. asotus Linnaeus): structure, phylogeny, and intraspecific variation. Genet Mol Res. 14:18198–18209. [DOI] [PubMed] [Google Scholar]
- Xia H, Liu L, Han Q, Yang P, Luo W, Wang W, Wu X, Chen N, Chen F, Zhou Y. 2018. Comparative analysis of non-specific immunity of Clarias fuscus, Silurus asotus, and Silurus meridionalis. ArXiv. 70:1–8. [Google Scholar]