Table 2.
Potential viral and host taxonomy for circular viral Uncultivated Viral Genomes (UViGs) and integrated prophages identified from SAS2A metagenomes. Putative host rank was identified by ViPTree proteomic analyses for circular genomes through closely related genomes (within the same clade) (Supplementary Figure S2) and rRNA 16S extracted genes for prophages. Viral taxonomy was inferred from ViPTree. For prophages, BLASTx hits are presented for similar hits, when available. UViGs are identified as either circular (C) or prophages (P), length is given in base pairs (bp) and CDS are protein-coding features. Normalized abundance was averaged across triplicates within a season.
| Genome Type | UViG | Contig ID | Length (bp) | Number of CDS | Summer Average Abundance | Winter Average Abundance | Putative Host Rank | Putative Viral Group |
|---|---|---|---|---|---|---|---|---|
| C | UViG3 | Ga0256681_10553986 | 63,992 | 103 | 2.18 | 0 | Firmicutes | Siphoviridae, Myoviridae (Lactococcus, Clostridium, Geobacillus phages) |
| C | UViG4 | Ga0256681_10542696 | 34,037 | 36 | 7.61 | 0 | ||
| C | UViG5 | Ga0256681_10559173 | 36,339 | 61 | 13.3 | 0 | Gammaproteobacteria | Podoviridae (Rhodoferax, Vibrio, Thalassomonas phages) |
| C | UViG6 | Ga0256681_11263128 | 62,403 | 87 | 4.54 | 0 | Gammaproteobacteria | Podoviridae, Sophoviridae (Pseudomonas, Xanthomonas phages) |
| C | UViG7 | Ga0256681_10579994 | 45,748 | 55 | 1.65 | 0 | ||
| C | UViG8 | Ga0256681_10547172 | 42,853 | 61 | 8.45 | 0 | Gammaproteobacteria | Podoviridae (Acetinobacter, Aeromonas, Edwardsiella phages) |
| C | UViG9 | Ga0256681_10567549 | 36,908 | 45 | 6.1 | 0 | Gammaproteobacteria | Podoviridae, Siphoviridae (Pseudoalteromonas, Marinomonas, Escherichia viruses) |
| C | UViG10 | Ga0256681_10572110 | 37,103 | 56 | 1.63 | 0 | Alphaproteobacteria | Siphoviridae (Caulobacter viruses) |
| C | UViG11 | Ga0256681_10539383 | 83,527 | 107 | 5.85 | 2.16 | Unknown | Unknown (Natrinema, Haloarcula viruses) |
| P | vOTU12 | Ga0256681_11878260 | 12,576 | 13 | 0.37 | 0 | Gammaproteobacteria | Myoviridae (Pseudomonas, Stenotrophomonas, Xanthomonas phage) |
| P | vOTU13 | Ga0256681_12578680 | 29,945 | 13 | 9.71 | 0 | (Bacteria, Archaea) | Unknown |
| P | vOTU14 | Ga0256681_12584168 | 15,432 | 17 | 1.2 | 0 | Unknown | Unknown |
| P | vOTU15 | Ga0256681_12612509 | 13,421 | 11 | 2.46 | 0 | (Bacteria) | Unknown |
| P | vOTU16 | Ga0256681_10168136 | 15,698 | 15 | 0.89 | 0 | Betaproteobacteria (Polaromonas) | Unknown |
| P | vOTU17 | Ga0256681_10683670 | 11,399 | 18 | 3.47 | 0 | Unknown | Unknown |
| P | vOTU18 | Ga0256681_11428677 | 14,176 | 18 | 1.33 | 0 | (Bacteria, Archaea) | Unknown |
| P | vOTU21 | Ga0256681_10088446 | 10,785 | 11 | 9.7 | 0 | (Bacteria, Archaea) | Unknown |