Table 2.
RT-qPCR primer sequences (5ʹ to 3ʹ).
| Transcript | Forward Primer | Reverse Primer | Annealing Temp. (°C) |
|---|---|---|---|
| IL-1β | TCATTGTGGCTGTGGAGAAG | GGTGTGCCGTCTTTCATTAC | 53.8 |
| TNF-α | CTCAGATCATCTTCTCAA | CAGAGCAATGACTCCAAA | 55.1 |
| TGF-β | GAAAGCCCTGTATTCCGTCTCCTT | CAACAATTCCTGGCGTTACCTTGG | 53.8 |
| IFN-γ | AGCGGCTGACTGAACTCAGATTGTA | GTCACAGTTTTCAGCTGTATAGGG | 61.2 |
| IL-6 | TTCCCTACTTCACAAGTC | GGTTTGCCGAGTAGATCT | 52.9 |
| IL-23 | CAAGCAGAACTGGCTGTTGTC | GCACCAGCGGGACATATGAA | 60.2 |
| MMP-9 | CACAACCGACGACGACGAGTTGTG | CTGTGGTGAGGCCGAATAG | 65.0 |
| β-Actin | TTGTTACCAACTGGGACGACATGG | GATCTTGATCTTCATGGTGCTAGG | 59.2 |
| GAPDH | ATGGTGAAGGTCGGTGTG | ACCAGTGGATGCAGGGAT- | 58.0 |
IL-1β, interleukin 1 beta; TNF-α, Tumor necrosis factor alpha; TGF-β, Transforming growth factor beta; IFN-γ, Interferon gamma; IL-6, interleukin 6; IL-23, interleukin 23; MMP-9, Matrix metallopeptidase 9.