FIG 6.

Exogenous PQS rescues ΔpqsR dispersion defect. PA14 wild-type and ΔpqsR biofilms were grown in semibatch cultures in 24-well plates for 4 days. For the following 2 days, the medium was exchanged every 12 h with fresh medium containing 40 μM PQS (+ PQS) or an equivalent amount of methanol (+ MeOH, vehicle control). Dispersion efficiency was then quantified for the strains under each condition. Error bars represent the standard deviations calculated from at least three biological replicates. Statistical significance was analyzed by ANOVA followed by Tukey’s post hoc test. n.s., P > 0.5; *, P < 0.05.