The authors wish to make the following changes to their paper [1].
We regret to have to inform the reader about a series of errors which relate to the control data set, which describes the basic observation of spontaneous ultraslow Na+ fluctuations in the hippocampus of neonates. This error arose because the authors originally split this data set of controls, but later on decided that the full set of data would be put into the manuscript, thereby increasing the number of control observations. Unfortunately, in the entire text as well as in Table 2 and Figure 1C, Figure 3B, and Figure 4A,B, the authors mistakenly reported the control values for one of the split data sets.
Table 2.
Neurons | Astrocytes | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cells (n) | % Cells (n) Active | Fluctuation (n) | mM Mean | mM SD | p Values | Min Mean | Min SD | Cells (n) | % Active | Fluctuation (n) | mM Mean | mM SD | p Values | Min Mean | Min SD | |
Control | 412 | 21.6 | 166 | 2.2 | 1.2 | 9.2 | 4.1 | 213 | 43 | 166 | 2.1 | 1.4 | 8.6 | 3.2 | ||
TTX | 94 | 7.4 | 7 | 0.7 | 0.3 | ** | 8.2 | 3.7 | 39 | 35.9 | 23 | 1 | 0.3 | *** | 8.7 | 4.1 |
BIC | 76 | 19.7 | 32 | 0.8 | 1 | *** | 6.6 | 2.3 | 22 | 59.1 | 20 | 2.7 | 1.9 | 0.06 | 8.1 | 2.3 |
CGP | 101 | 12.9 | 20 | 2 | 0.6 | 0.48 | 10.9 | 3.5 | 49 | 44.9 | 42 | 2.1 | 1.2 | 0.78 | 9.6 | 4.6 |
SNAP/NNC | 53 | 22.6 | 17 | 1.1 | 0.2 | *** | 10.2 | 3.1 | 86 | 34.9 | 33 | 3.2 | 2.0 | ** | 10.8 | 6.2 |
NPA | 70 | 15.7 | 20 | 1 | 0.3 | *** | 10.7 | 3.8 | 43 | 32.6 | 31 | 1.3 | 0.6 | ** | 9.4 | 3.6 |
Sarcosine | 74 | 20.3 | 20 | 1.5 | 0.5 | 0.02 | 11.3 | 3.7 | 62 | 21 | 25 | 2.6 | 1.7 | 0.06 | 8.5 | 3.5 |
TFB-TBOA | 36 | 27.8 | 10 | 1.9 | 1.7 | 0.69 | 8.5 | 3.7 | ||||||||
CNQX | 130 | 6.2 | 9 | 2.6 | 1.3 | 0.29 | 9.6 | 4.1 | 45 | 37.8 | 25 | 2.1 | 1.1 | 0.86 | 8.9 | 3.1 |
APV | 156 | 10.3 | 19 | 2.5 | 0.9 | 0.22 | 10.9 | 5.2 | 52 | 28.8 | 19 | 2.7 | 2.5 | 0.34 | 13.1 | 4.9 |
MPEP | 117 | 16.2 | 30 | 1.9 | 0.7 | 0.28 | 9.5 | 3.8 | 26 | 46.2 | 53 | 3.9 | 2.2 | 0.36 | 9.1 | 6.2 |
α-BT | 118 | 11.9 | 29 | 1.6 | 0.6 | 0.02 | 10.1 | 3.7 | 43 | 67.4 | 89 | 2 | 1.5 | 0.93 | 7.3 | 3.7 |
Atropine | 124 | 16.1 | 28 | 2.6 | 1.3 | 0.14 | 8.5 | 2.9 | 73 | 49.3 | 74 | 2.5 | 1.2 | 0.02 | 7.7 | 2.9 |
PPADs | 70 | 11.4 | 19 | 1.4 | 0.9 | 0.01 | 10.7 | 4.9 | 57 | 24.6 | 24 | 1.6 | 0.6 | 0.1 | 8 | 2.4 |
Caffeine | 142 | 22.5 | 75 | 1.9 | 0.7 | 0.05 | 9.4 | 4.8 | 32 | 62.5 | 33 | 2 | 0.9 | 0.84 | 8.8 | 3.2 |
Prazosin | 111 | 13.5 | 21 | 1.3 | 0.4 | ** | 9.7 | 3.7 | 81 | 40.7 | 50 | 1.7 | 0.8 | 0.18 | 10.4 | 4.6 |
Propranolol | 123 | 7.3 | 11 | 1.9 | 0.5 | 0.55 | 11.4 | 4.3 | 59 | 38.9 | 54 | 2.7 | 1.2 | 0.01 | 8.7 | 4.1 |
SEA0400 | 85 | 11.7 | 12 | 2 | 1.3 | 0.65 | 8.8 | 3.1 | 18 | 44.4 | 9 | 2.6 | 1 | 0.21 | 8 | 1.4 |
Bumet | 132 | 6.1 | 8 | 1.8 | 0.5 | 0.44 | 11 | 3.1 | 29 | 37.9 | 15 | 1.6 | 1.3 | 0.28 | 8.5 | 3.5 |
Amiloride | 162 | 11.1 | 31 | 1.8 | 0.6 | 0.11 | 8.7 | 2.7 | 50 | 28 | 20 | 1.7 | 0.5 | 0.34 | 8.8 | 3.5 |
La3+ | 144 | 5.2 | 8 | 1.8 | 0.6 | 0.43 | 11.9 | 5.3 | 84 | 40.5 | 74 | 2.0 | 1.2 | 0.71 | 8.4 | 3.4 |
C57Bl/6 WT | 460 | 11.1 | 66 | 1.5 | 0.8 | 10.8 | 4.9 | 548 | 51.1 | 620 | 2.1 | 1.5 | 8.7 | 4.0 | ||
Cx -/- | 77 | 18.2 | 30 | 1.6 | 0.6 | 0.65 | 11.4 | 5.6 | 46 | 60.1 | 58 | 2.2 | 1.5 | 0.77 | 8.7 | 4.3 |
Abbreviations, see Table 1. Bumet: bumetanide; a-BT: a-bungarotoxin. Red color indicates statistically significant difference as compared to the control condition, p-values indicated apply to the amplitudes; ** 0.001 ≤ p < 0.01, *** p < 0.001.
In the Abstract, “we found that 20% of pyramidal neurons and 44% of astrocytes” should be replaced with “…we found that 22% of pyramidal neurons and 43% of astrocytes…”.
In Section 3.1, in the first paragraph, “…were present in 44% of recorded astrocytes (n = 51/116 cells; Table 2) and in 20% of recorded neurons (n = 49/234 cells; Table 2, Figure 1C). The frequency of these fluctuations was very low, with the active astrocytes averaging at 1.8 ± 1.1 fluctuations/hour and the active neurons exhibiting 1.5 ± 0.9 fluctuations/hour.” should be replaced with “…were present in 43% of recorded astrocytes (n = 92/213 cells; Table 2) and in 22% of recorded neurons (n = 89/412 cells; Table 2, Figure 1C). The frequency of these fluctuations was very low, with the active astrocytes averaging at 1.8 ± 1.2 fluctuations/hour and the active neurons exhibiting 1.9 ± 1.3 fluctuations/hour”.
In the third paragraph, “…average duration of 8.7 ± 3.4 min in astrocytes (n = 91 fluctuations; Table 2) and 9.2 ± 4.2 min in neurons (n = 116 fluctuations; Table 2). The mean amplitude for fluctuations was 2.1 ± 1.3 mM and 2.2 ± 1.2 mM for astrocytes…” should be replaced with “…average duration of 8.6 ± 3.2 min in astrocytes (n = 166 fluctuations; Table 2) and 9.2 ± 4.1 min in neurons (n = 166 fluctuations; Table 2). The mean amplitude for fluctuations was 2.1 ± 1.4 mM and 2.2 ± 1.2 mM for astrocytes…”.
In Section 3.2, in the second paragraph, “, dropping in astrocytes from 44% (n = 116) in neonatal animals…” should be replaced with “…dropping in astrocytes from 43% (n = 213) in neonatal animals…”; “, starting at 20.1% in neonates (n = 234) and staying at” should be replaced with “, starting at 21.6% in neonates (n = 412) and staying at…”. In the third paragraph, “…mean frequency (signals/hour) of 1.8 ± 1.1, 1.7 ± 0.8, 1.4 ± 0.7, and 1.1 ± 0.3 for astrocytes and 1.5 ± 0.9, 2.6 ± 1.6…” should be replaced with “a mean frequency (signals/hour) of 1.8 ± 1.2, 1.7 ± 0.8, 1.4 ± 0.7, and 1.1 ± 0.3 for astrocytes and 1.9 ± 1.3, 2.6 ± 1.6”.
In Section 3.5, in the first paragraph, “…we observed an increase in astrocyte amplitudes (2.9 ± 1.8 mM; p < 0.001).” should be replaced with “we observed an increase in astrocyte amplitudes (2.9 ± 1.8 mM; p < 0.002)”.
Correspondingly, Figure 1 should be replaced with the following:
Table 2 should be replaced with the following:
Figure 3 should be replaced with the following:
Figure 4 should be replaced with the following:
Figure 5 should be replaced with the following:
The authors would like to apologize for any inconvenience caused to the readers by these changes. The changes do not affect the scientific results. The manuscript will be updated and the original will remain online on the article webpage, with a reference to this correction.
Footnotes
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References
- 1.Felix L., Ziemens D., Seifert G., Rose C.R. Spontaneous Ultraslow Na+ Fluctuations in the Neonatal Mouse Brain. Cells. 2020;9:102. doi: 10.3390/cells9010102. [DOI] [PMC free article] [PubMed] [Google Scholar]