Table 2.
Bioactive properties of bioaccessible compounds from Tannat grape skin hydro-alcoholic-acid extract (digest).
| Digest | Assays | ||
|---|---|---|---|
| (µg/mL) | Antioxidant Properties | ||
| Intracellular ROS Formation in CCD-18Co Cells (%) | |||
| Physiological Conditions | Induced by t-BOOH (1 mM) | ||
| Prevention | Prevention with Co-Administration | ||
| 0 | 100.0 ± 6.3 a,A | 151.0 ± 12.7 a,b,B | 178.0 ± 7.0 b,B |
| 100 | 152.5 ± 39.2 b,A | 146.4 ± 30.5 a,b,A | 117.9 ± 30.0 a,A |
| 250 | 125.8 ± 13.7 a,b,A | 174.7 ± 57.2 b,A | 128.6 ± 21.2 a,A |
| 500 | 144.1 ± 10.4 b,A | 122.8 ± 13.8 b,A | 138.8 ± 32.0 a,A |
| 1000 | 135.1 ± 24.8 a,b,A | 192.4 ± 19.6 b,B | 185.3 ± 19.0 b,B |
| Intracellular ROS Formation in RAW 264.7 Cells (%) | |||
| Physiological Conditions | Induced by t-BOOH (1 mM) | ||
| Prevention | Prevention with Co-Administration | ||
| 0 | 100.0 ± 8.3 a,A | 211.4 ± 44.4 b,B | 211.4 ± 44.4 b,B |
| 100 | 98.8 ± 20.0 a,A | 136.6 ± 15.8 a,B | 150.1 ± 39.3 a,B |
| 250 | 143.2 ± 39.1 b,A | 132.2 ± 10.9 a,A | 110.8 ± 21.4 a,A |
| 500 | 99.3 ± 24.3 a,A | 150.2 ± 21.1 a,B | 124.8 ± 16.6 a,A,B |
| 1000 | 116.3 ± 44.8 a,b,A | 99.4 ± 27.9 a,A | 129.9 ± 46.4 a,A |
| Anti-inflammatory Properties | |||
| (µg/mL of NO Formation in RAW 264.7 Cells Induced by LPS 1 µg/mL) | |||
| Prevention | Prevention with Co-Administration | ||
| 0 | 9.9 ± 0.8 c,A | 9.9 ± 0.8 c,A | |
| 100 | 5.3 ± 0.7 b,A | 5.6 ± 0.9 b,A | |
| 250 | 4.1 ± 0.9 a,A | 5.4 ± 1.0 b,B | |
| 500 | 3.3 ± 0.6 a,A | 4.9 ± 0.7 a,b,B | |
| 1000 | 3.1 ± 1.1 a,A | 4.0 ± 1.2 a,A | |
Results are expressed as mean values ± SD (n = 3). Different letters indicate significant differences (Tukey test, p < 0.05) between values in the same column (in lower case) or in the same row (capital letters). All determinations were performed in triplicate in three different cell passages.