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. 2020 Oct 30;8(11):1700. doi: 10.3390/microorganisms8111700

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Pf4 phage variant infection triggers a strong SigX-mediated cell envelope stress response in H103 strain. (A). Relative mRNA expression levels of sigX, cfrX, cmpX, fabY, PA1181, PA2072 and gcbA in H103 (green bars) and Pf4*-T (violet bars) conditions as determined by RT-qPCR experiments. (B). Membrane fluidity assessment by fluorescence anisotropy using 1,6-diphenyl-1,3,5-hexatriene (DPH) probe in H103 (green bar) and Pf4*-T (violet bar) conditions. Each experiment was assayed at least four times independently. Statistics were achieved by paired (two samples) two-tailed t-test. ^NS p > 0.05; * p < 0.05; *** p < 0.001; **** p < 0.0001.