Figure 6.

Pf4 phage variant infection leads to an AlgU-mediated cell envelope stress response in H103 strain. (A). Relative mRNA expression of algU, algD, algR, cmaX, mucR and mreB in H103 (green bars) and Pf4*-T (violet bars) conditions as determined by RT-qPCR experiments. (B). Sequence of the DNA region upstream of mreB showing a predicted AlgU-dependent promoter and σ⁷⁰-dependant promoter. (C). Relative mRNA expression levels of mreB in P. aeruginosa H103 (green bar) and ΔalgU (yellow bar) as determined by RT-qPCR experiments. Each experiment was assayed at least four times independently. Statistics were achieved by paired (two samples) two-tailed t-test. ^NS p > 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.