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. 2020 Oct 28;10(11):1488. doi: 10.3390/biom10111488

Table 1.

Primers sequence used for real-time PCR of toll-like receptor (TLR) pathway gene expression.

TLR Pathway Mediators Primer Sequence (5′ to 3′)
Forward Primer Reverse Primers
TLR4 AGTGTATCGGTGGTCAGTGTGCT AAACTCCAGCCACACATTCC
MyD88 GAGATCCGCGAGTTTGAGAC CTGTTTCTGCTGGTTGCGTA
TRIF TCAGCCATTCTCCGTCCTCTTC GGTCAGCAGAAGGATAAGGAA
Bax AGACACCTGAGCTGACCTTGGA CGCTCAGCTTCTTGGTGGAT
Bcl2 GGGATGCCTTTGTGGAACTATATG CAGCCAGGAGAAATCAAACAGA
HMGB-1 AGGCTGACAAGGCTCGTTATG TGTCATCCGCAGCAGTGTTG
β-Actin CACGATGGAGGGGCCGGACTCATC TAAAGACCTCTATGCCAACACAGT