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. 2020 Nov 18;8(11):513. doi: 10.3390/biomedicines8110513

Table 1.

RT-PCR primer sequences for tested genes.

Gene Forward Primer Sequence
(5′→3′)		 Reverse Primer Sequence
(5′→3′)
GAPDH 5′-CTTTTGCAGACCACAGTCCATG-3′
(22 bases)		 5′-TTTTCTAGACGGCAGGTCAGG-3′
(21 bases)
iNOS 5′-GGAGCAACGTTGAGGAAATAAGACT-3′
(25 bases)		 5′-AAGAGCCAGAAGCGCTATCAC-3′
(21 bases)
CD206 5′-GGAGGATTCCATGTATTTGTGAGC-3′
(24 bases)		 5′-AAATGAGTGAAGTGAAATCAGTTACCT-3′
(27 bases)
TNF-α 5′-TCTCCCTCAAGGACTCAGCTTTCTG-3′
(25 bases)		 5′-TGAGAGGAAGAGAACCTGCCTGG-3′
(23 bases)
IL-6 5′-CAGGAGAAGATTCCAAAGATGTAGCC-3′
(26 bases)		 5′-TGCTCTAGAACCCAGCAAAGAC-3′
(22 bases)
IL-10 5′-ATGTCTAGTTCAGGCAGTCCCA-3′
(22 bases)		 5′-GGGCTTGCTCTTGCAAAACC-3′
(20 bases)