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. 2020 Nov 18;25(22):5392. doi: 10.3390/molecules25225392

Table 1.

Representative purification of DPP4 from Sf9 cells.

Volume (mL) Protein (mg/mL) Total Protein (mg) Total Activity (U) Specific Activity (U/mg) Fold-Purification * Yield (%) ** Step-Wise DPP4 Loss (%) # Step-Wise Total Protein Loss (%) ##
Cell culture supernatant 1000 7.3 7300 144 0.020 1 100 0 0
35% AS 1046 3.9 4079 109 0.027 1.4 76 24 44
80% AS 82 7.2 589 85 0.14 7.0 59 22 86
Phenyl Sepharose 93 4.1 381 77 0.20 10 53 9.4 35
Ni Sepharose 41 0.034 1.4 64 46 2300 44 16 99.6
DEAE Sepharose 4.4 0.23 1.0 42 42 2100 29 35 29

* Fold-purification is specific activity after the step/specific activity of the starting material (cell culture supernatant). ** Yield is Units of DPP4 as a % of DPP4 Units in the starting material. # Step-wise DPP4 loss (%) is 100 × (total activity after the previous step − total activity after this step)/total activity after the previous step. ## Step-wise total protein loss (%) is 100 × (total protein after previous step − total protein after this step)/total protein after the previous step.