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. 2020 Nov 19;10(11):1574. doi: 10.3390/biom10111574

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Determination of total polyphenols content (TPC) and its stability in evening primrose extracts (EPE) determined by the Folin–Ciocalteu method. (A) EPEs obtained in continuous Soxhlet extraction procedures performed for 6, 12, 24, and 48 h (EPE6, EPE12, EPE24, and EPE48, respectively). (B) Stability of EPE was determined by comparing the content immediately after extraction, as well as 1 and 3 years after extraction. TPC expressed in mg of GA per gram of dried extract. Data are means of three independent experiments ± SD. * p < 0.05 compared to control.