Colloquium Correction for “Humoral immune response to native eukaryotic prion protein correlates with anti-prion protection,” by Magdalini Polymenidou, Frank L. Heppner, Erica C. Pellicioli, Eduard Urich, Gino Miele, Nathalie Braun, Franziska Wopfner, Hermann M. Schätzl, Burkhard Becher, and Adriano Aguzzi, which was first published August 3, 2004; 10.1073/pnas.0404772101 (Proc. Natl. Acad. Sci. U.S.A. 101, 14670–14676).
The authors note “It has recently come to our attention that there is an error in Fig. 2 of our manuscript. The experiment described in Fig. 2 consisted of 44 samples analyzed by fluorescence-activated cell sorting (FACS), including four biological replicates for each experimental group. The published Fig. 2 was compiled by a single, representative image from each experimental group, which was chosen randomly. The panel in the Upper Right corner of the published figure (Prnpo/o serum on tg33 cells) was accidentally duplicated in the Lower Left panel (6H4μ × Prnpo/o serum on Prnpo/o cells) during preparation of the figures.” The corrected Fig. 2 and its legend appear below.
Fig. 2.
Sera from 6H4μ transgenic mice react with PrPC on the surface of tg33 T cells. Scattergrams show representative FACS analyses of lymphocytes (tg33 or Prnpo/o) stained with mouse sera. 6H4μ transgenic sera react with PrPC overexpressing T cells (tg33; Upper), but not with Prnpo/o T cells (Lower). PrPC gene dosage in 6H4μ mice correlates negatively with the extent of binding to PrPC, suggesting the occurrence, to some extent, of clonal deletion of auto-reactive B cells.