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. Author manuscript; available in PMC: 2021 Mar 10.
Published in final edited form as: Nature. 2020 Sep 10;587(7835):673–677. doi: 10.1038/s41586-020-2749-z

Extended data Figure 5. Cryo-EM analysis of mcGAS domain in complex with nucleosome purified from HEK 293T cells.

Extended data Figure 5.

a. Representative micrograph of mcGAS-nucleosome complex in vitrified ice. The scale bar denotes 50 nm.

b. 2D class averages of mcGAS-nucleosome complex particles. The scale bar denotes 10 nm.

c. Flowchart of data processing; see Methods for details.

d. Angular distribution of particles included in the final reconstruction.

e. Final 3D reconstruction, colored according to the local resolution.

f. Corrected Gold-standard Fourier shell correlation curves for the 3D electron microscopy reconstruction.

g. Density map (contoured at 3 σ) and structural model of cGAS-nucleosome (1:1) complex.