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. 2020 Dec 1;15(12):e0243089. doi: 10.1371/journal.pone.0243089

Demography and productivity during the recovery time sequence of a wild edible bamboo after large-scale anthropogenic disturbance

Noboru Katayama 1,¤a,*, Osamu Kishida 1,¤b, Chikako Miyoshi 1, Shintaro Hayakashi 1, Kinya Ito 1, Rei Sakai 1, Aiko Naniwa 1, Hiroyuki Takahashi 1, Kentaro Takagi 1
Editor: Xiao Guo2
PMCID: PMC7707573  PMID: 33259530

Abstract

Anthropogenic disturbances in forest management practices can affect wild edible plants. Soil scarification is a large-scale disturbance that may cause long-term reduction in productivity of edible dwarf bamboo, Sasa kurilensis, in northern Japan. For their effective and sustainable use, we need to understand the recovery process after such disturbances. At 14 study sites in the Teshio Experimental Forest of Hokkaido University where soil scarification had been conducted between 2 and 44 years prior, the number and stem diameter of old and young (newly emerged, edible) culms was recorded. At sites that were within 11 years of soil scarification, the proportion of old culms (<11%) was lower than in the control area where soil scarification had never been conducted. At sites where more than 15 years had passed since soil scarification, the relative number of old culms was nearly equal to that in control area. Additionally, the number of young culms increased with an increasing number of old culms. These results suggest that recovery of productivity (in term of number) of edible culms may take a few decades. In contrast, the culm diameter of young culms increased linearly with time since soil scarification, but the 95% confidence interval in this relationship suggests that dwarf bamboo can produce thick edible culms soon after soil scarification. These findings will provide useful insights into how to obtain high quality bamboo culms following anthropogenic disturbances in future.

Introduction

Humans obtain a variety of ecosystem services from ecological environments, e.g., supply and regulation of water, retention and accumulation of soil, and carbon and other elemental storage [1]. Ecosystem services of stably maintained ecological environments can be sustainably utilized; however, natural and anthropogenic disturbances to ecological environments alter the stability of these environments. Intense impact disturbances that exceed the ecological resilience sometimes cause critical damage to ecosystem functioning, which leads to a dramatic decline in ecosystem services [2, 3]. In particular, large-scale human-induced impacts to forests have intensified worldwide in recent decades, and there is increasing concern about the influence of anthropogenic disturbances on forest ecosystems [4, 5]. As humans have come to depend on forest resources through the ages, understanding the impacts of anthropogenic disturbances on ecosystem services in forests is a critical issue.

Provisioning services are one of the major fundamental services in forest ecosystems [6, 7]. Forests provide not only timber products, but also non-timber forest products (NTFPs) such as ecological medicines (i.e., wild medical herbs) and foodstuff (i.e., wild edible plants). NTFPs have been primary medicines and essential foods for humans since ancient times and remain as an important source of income in many nations [8, 9]. While NTFPs principally grow without management by humans, their productivity is greatly influenced by human activities [10, 11] and also depends on environmental conditions [12, 13].

A number of NTFPs are known to be disturbance-dependent species, which are favored to appear in habitats following natural disturbances [6]. Additionally, NTFPs with high compensatory growth can show increased productivity following harvest by humans [14]. Nevertheless, large-scale anthropogenic disturbances, such as over-utilization and/or forest practices, can drastically decrease the abundance and habitat of NTFPs. For sustainable utilization of NTFP resources, we need to understand how large-scale anthropogenic disturbances influence the demography and productivity of NTFPs over time. In this study, we examine the long-term effects of large-scale anthropogenic disturbances on edible dwarf bamboo, Sasa kurilensis, based on demography and productivity of the bamboo.

Dwarf bamboos (genus Sasa) are prominent understory species in East Asian forests, including Japan [15–17]. Eating newly emerged bamboo culms is part of Asian food customs, and new culms of S. kurilensis (but not S. senanensis) are one of the most popular wild edible plants in northern Japan. In Hokkaido, the northernmost island of Japan, S. kurilensis and S. senanensis are the dominant dwarf bamboo species and cover over 89% of the forest area [18]. Sasa quickly expands its range widely over forest areas by extending vegetative culms (ramets) [19]. The culms can reach over 2 m in height, completely covering the understory in some cases, and dwarf bamboos have been targeted for eradication because thick stands of dwarf bamboos strongly inhibit establishment and growth of seedlings of commercial trees [20, 21].

Soil scarification has been conducted in Hokkaido, Japan since the mid-20th century to remove understory vegetation together with dwarf bamboo. Soil scarification is a forestry practice of peeling off the soil surface using civil engineering machinery [22, 23], and in most cases, this process leads to the establishment of secondary forests dominated by birches, Betula spp., after exposing mineral soil [22, 24]. While there is a growing body of evidence that soil scarification leads to the successful establishment of secondary forests [22, 23, 25, 26], knowledge about the demographic patterns of dwarf bamboo after soil scarification remains limited. Moreover, little is known about how long it takes to recover productivity of edible culms after soil scarification.

The objective of this study is to demonstrate the recovery process of the demography and productivity of S. kurilensis after soil scarification. We carried out a field survey in the Teshio Experimental Forest of Hokkaido University, Japan, where soil scarification had been conducted since the 1970s with a record of when and where it had been conducted. In this survey, we selected 14 study sites (disturbed areas) where soil scarification had been conducted between 2 and 44 years previously, and we measured the number and culm diameter (the stem diameter) of both old and young (newly emerged, edible) bamboo culms as demographic and productivity indexes. Because environmental factors influence the demography and productivity of bamboo, we cannot directly evaluate the time sequence of the recovery process. Thus, we also investigated the demography and productivity of bamboo in neighboring control areas where scarification had not been conducted. Based on analysis of the paired study areas (i.e., disturbed areas vs. control areas), we estimated the duration of recovery for the bamboo population.

Materials and methods

Materials

S. kurilensis grows in the northern mountainous regions of Japan. Old culms reach a height of about 1.5 to 3 m, and individual culms (ramet) have a lifespan of approximately 8 years, and the individual dwarf bamboo (genet) may have a lifespan of more than 60 years [27, 28]. In early June in northern Japan, several new culms, which are edible, emerge around the old culms. These are known as 'Hime-takenoko' or 'Nemagari-take' and are commercially traded wild vegetables in Japan.

Study sites

Study sites were selected in the Teshio Experimental Forest of Hokkaido University (44°54’–45°06’N, 141°56’–142°10’E; area: 22,517 ha), which is located in the northeast forest zone of Japan where the mean annual temperature is 5°C (max. 35°C, min. −35°C) and the annual precipitation is approximately 1000 mm [29]. While the forest overstory mainly consists of deciduous (e.g., Betula ermanii) and conifer trees (e.g., Picea jezoensis), the understory includes stands of dwarf bamboos (S. kurilensis and S. senanensis). The stands of S. kurilensis and S. senanensis are usually monocultures, and they are not comingled in our study sites (N. Katayama, personal observation). Multiple forest fires and various forestry practices, such as soil scarification, had been conducted during the last and current centuries at these sites.

In this study, we chose 14 study sites (1–14) in the Teshio Experimental Forest based on GPS-linked data documenting where and when soil scarifications (anthropogenic disturbance) had been conducted from 1972 to 2014 (S1 Table and S1A Fig). Field surveys were conducted in 2016, which was 2 to 44 years after soil scarifications. Disturbed areas at these sites were essentially untouched after scarification without forestry practice intervention, harvest of old and young bamboo culms or forest fires. Ecological succession in each disturbed area resulted in establishment of stands of birch trees with S. kurilensis (S1B–S1E Fig).

Field surveys

We investigated demography and changes in productivity of S. kurilensis, using the 14 study sites (S1 Table and S1A Fig). In July 2016, we set four survey plots (1 × 1 m2) at 10 m inside the boundary of the disturbed areas at study sites 1–9 where more than 11 years had passed since scarification. The plots were separated by at least 5 m. Because the density of bamboo culms was extremely low at study sites 10–14 where less than 6 years had passed after soil scarification (see Results), we set 10 survey plots (1 × 1 m2) in the disturbed areas at these sites in order to obtain sufficient observations of bamboo culms for statistical analyses. Then, we counted the number of bamboo culms in each plot. In this survey, we classified bamboo culms into 'old' (emerged before the current year) and 'young' (emerged in the current year). The number of old culms was considered to indicate the potential for the bamboos to produce edible culms because the density of old culms positively correlated with the number of edible culms [14] (and see Results). Young culms were regarded as the equivalent of edible culms produced in the current year. In this survey, we measured the stem diameter of all young culms to estimate the quality (culm diameter) of edible culms as culms with greater diameter are generally classified as being more highly qualified as food items. Further, to examine the relationship between the culm diameters of old and young culms, we also measured the stem diameter of 4 old culms, which were randomly selected in each plot (16 culms per area). If there were fewer than 4 old culms in the plot, the stem diameter was measured for all old culms.

To evaluate the demography and productivity of bamboo culms after minimizing any confounding effects due to environmental conditions, we conducted the same survey in 'control areas' at study sites (14 control areas, in total) located within 100 m from the edge of each disturbed area. Based on the GPS-linked data, we chose areas with 'no records' of anthropogenic disturbance including soil scarification and bamboo-harvesting, set four survey plots in each control area, and measured the number and stem diameter of old and young culms by the same method mentioned above. The "original" conditions of old (mother) bamboos would not be different between treatment groups (i.e., disturbed areas vs. control areas) because the number and diameter of old culms, which strongly reflect differences in environmental conditions (N. Katayama, unpublished data), did not differ between treatment groups at more than 24 years after soil scarification (see Results).

Statistical analysis

To examine changes in the number and stem diameter of old and young culms after soil scarification, we applied general linear mixed models (GLMMs) with an assumption of normal distribution using treatments (control vs. disturbance) and time after the soil scarification as fixed factors. In these models, site identity was used as a random factor. Because we detected significant interactions between time and treatment (see Results), we individually conducted GLMMs to examine the time sequence process on each independent factor (i.e., the numbers and culm diameter of culms) in each treatment (i.e., control and disturbance).

To examine the recovery process of the bamboo after soil scarification in more detail, we calculated the log response ratio (ln[treatment/control]) using numbers and diameters of both old and young culms in the time sequence. The log response ratio is widely used to compare the effect magnitude in manipulation experiments [30], and in our study, to correct for the effects of study site locations (i.e., unknown environmental factors derived from the site locations) on each independent factor. A log response ratio of <0 is taken as a negative effect relative to the control, and conversely, a log response ratio of >0 indicates a positive effect. To calculate the mean and 95% confidence interval (CI) of the log response ratio of the numbers of old and young culms, separate bootstrap models were used with 1000 resampling iterations at each study site. In these models, the number of culms in the disturbed plots (scarification plots) was used as the treatment value and that in the corresponding control plots was taken as the control value. To avoid 0 in the denominator and numerator, 0.1 was added to each value. In addition, we calculated mean and 95% CI of log response ratios of culm diameter using similar separate bootstrap models. In the bootstrap models, data of individual culms instead of the plot mean data were used because there were no or few culms in some plots. In this survey, we used stem diameter data of a total of 379 old and 229 young culms, but we could not carry out bootstrap models of diameter of young culms in disturbed areas at sites 10 and 14 due to too few young culms. Then, general additive models (GAMs) assuming normal distribution were constructed to demonstrate the non-linear relationships between the log response ratios and time since soil scarification using mean log response ratios and years as dependent and independent values, respectively.

To examine the relationships between the number of old and young culms per plot and between the culm diameter per plot, we conducted GLMMs using the site identity as a random factor.

The GLMMs and GAMs were carried out using JMP ver. 14 (SAS Institute Inc., Cary, NC, USA) and the R 'mgcv' package ver. 1.8.17 [31], respectively.

Results

While the number and diameter of both old and young culms increased significantly after soil scarification (S2 Table and S2 Fig), there were significant interactions between time and treatment (S2 Table). Based on the results of subsequent GLMMs separately analyzed for each treatment, we found that none of the dependent variables were affected by time in the control plots (S3 Table). However, values for each dependent variable in the disturbed plots were significantly increased with time (S3 Table).

In analyses of log response ratios, the GAM demonstrates that the fit smoothing spline (i.e., non-linear relationship) between the relative (i.e., log response ratio) number of old culms and years after soil scarification is a saturation curve (F2.2,2.7 = 41.07, P < 0.0001; Fig 1A), but the average values of the log response ratios were extremely low at sites within 11 years following soil scarification (<11%, compared to control area). The upper ranges of 95% CI of the log response ratios at these sites were lower than the neutral level (ratio = 0). On the other hand, the log response ratios did not significantly differ from 0 at sites surveyed more than 14 years after soil scarification (Fig 1A).

Fig 1. Log response ratios of number and culm diameter of old and young bamboo culms at 2 to 44 years after soil scarification.

Fig 1

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Error bars denote 95% CI from 1000 resampling iterations by bootstrap models, and solid curves indicate non-linear regressions by GAMs. Dotted lines indicate a neutral level of the effects of soil scarification (‘log response ratio’ = 0). The upper panels show plots of mean data, and the lower panels show individual culm data.

In addition, the relative number of young culms is related to time after soil scarification by a saturation curve (GAM, F2.7,3.3 = 15.29, P = 0.0001; Fig 1B). At sites surveyed within 6 years after soil scarification, the log response ratios were significantly lower than 0 except for at one site (site 11, 5 years after soil scarification), but the log response ratios were not significantly different from 0 at other sites (more than 11 years after soil scarification).

The relationship between the log response ratio of the diameter of old culms and years was also a saturation curve (GAM, F2.0,2.4 = 18.27, P < 0.0001; Fig 1C), and the log response ratios were significantly lower than 0 within 6 years after soil scarification, except at one study site (site 11) and were not different from 0 at sites more than 11 years after soil scarification. On the other hand, the log response ratio of the diameter of young culms gradually increased with time after soil scarification (Fig 1D), and fitting of the smoothing spline (GAM, F1,1 = 8.83, P = 0.0136) was approximately linear. Based on the range of 95% CI of the log response ratios, there was no significant effect of soil scarification on relative culm diameter, except at two sites: study site 9 at 11 years after soil scarification and study site 14 at 2 years after soil scarification.

There was a positive relationship between the number of old and young culms per plot (GLMM, F1,61.7 = 121.02, P < 0.0001; Fig 2A), as well as between the average diameter of old and young culms per plot (GLMM, F1,52.7 = 83.63, P < 0.0001; Fig 2B).

Fig 2.

Fig 2

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Relationships (a) between numbers of young and old culms and (b) between the diameter of young and old culms. Solid lines and grey zones denote linear regression and 95% CI, respectively. The dotted line on the lower figure has a slope of 1, suggesting no change across years. These figures plot the mean data. Note that several points in the upper figure overlap.

Discussion

Wild edible plants growing in natural fields are strongly influenced by anthropogenic disturbances, such as human harvesting [14, 32] and forest management practices [33–35]. For instance, removing the edible dwarf bamboo, S. kurilensis, during forest management practices (soil scarification) in northern Japan represents a large-scale disturbance that can subsequently decrease its productivity of edible culms for a long time. In this study, we evaluated the long-term impacts of soil scarification on the demography and productivity of S. kurilensis through a field survey conducted in a northern Japanese forest.

Our analysis of the log response ratios demonstrated that the recovery process of the number of old culms after soil scarification follows a saturation curve (Fig 1A), suggesting that soil scarification depresses the density of old culms for about 10 years. However, the density of old culms rapidly recovers after some point (by 14 years after soil scarification in our analysis). The density of young culms showed a similar pattern (saturation curve) but the trend was slightly weaker than the response for the density of old culms. To explain this difference in response, we should note that this result is partly a by-product in our statistical analysis; namely, since there was generally a greater number of old culms than young culms in control sites, the log response ratios of old culms tended to be more negative than that for young culms for some period after soil scarification. In any case, the productivity of young (edible) culms was related to the recovery in density of old culms (Fig 1A and 1B and S2A and S2B Fig), which is also indicated by the positive relationship between the number of old and young culms (Fig 2A). Because young bamboo culms emerge from the base of old culms [19, 28], the local density of old culms is a useful index of the productivity and recovery of edible culms in the site. In summary, dwarf bamboo can recover productivity (in terms of 'number' of young culms) within a few decades after soil scarification (Fig 1A and 1B).

A similar trend (saturation curve) was observed in the relative diameter of old culms (Fig 1C). On the other hand, the trend in relative diameter of young culms was a little different; the recovery pattern of the diameter of young culms was an approximately linear relationship (Fig 1D), and there were few sites where soil scarification had a significantly negative impact on the diameter of young culms (except at site 10 and site 14). These results suggest that dwarf bamboo could produce thick edible culms within a short period of time after soil scarification. Based on the positive relationship between the diameter of old and young culms per plot (Fig 2B), thicker old culms could produce thicker young culms. In addition, in term of 95% CI in this relationship, we can see that thin old culms (especially those with a diameter <10 mm) tended to produce slightly thicker young culms. In this process, bamboo stands with thin old culms would gradually establish stands with thicker young culms. Since the diameter of young culms is an important index of the quality of edible culms, the process of recovering the diameter of bamboo culms after soil scarification is essential for utilizing available ecosystem services from the dwarf bamboo.

While demographic patterns (population size and/or culm diameter) of S. senanensis are also likely to change (increase) after soil scarification, populations of S. senanensis and S. kurilensis are not comingled (i.e., they form monoculture stands) in our study sites. For this reason, we think that the demography of S. senanensis may have little effect on that of S. kurilensis, and vice versa.

Despite their importance, ecosystem services are rapidly degraded by multiple disturbances [36, 37]. In particular, large-scale anthropogenic disturbances can cause an unexpected change in the status and functioning of ecosystems and have a significant impact on ecosystem services over time [4]. Therefore, we need to understand the exact mechanisms by which disturbances influence the spatial and temporal variation of ecosystem services. In this study, we examined the demography and productivity during the recovery time sequence of an edible bamboo after soil scarification, which is an anthropogenic disturbance, and we demonstrated that recovery of the population size (density) of the dwarf bamboo culms lagged behind in the recovery of culm diameter, indicating that productivity of valuable edible culms may be low for some time after soil scarification.

The abundance of S. kurilensis is not declining at present. However, it is uncertain whether this abundance can be maintained in the future. If we look at NTFPs, sudden decline and extinction can occur after large-scale anthropogenic disturbances. Indeed, many NTFPs that were once dominant species have now become endangered due to anthropogenic disturbances (e.g., [7, 38, 39]). For better management, we should examine the potential impacts of anthropogenic disturbances on as many NTFPs (including S. kurilensis) as possible. Therefore, even at present, it is worthwhile to examine how S. kurilensis recovers following soil scarifications. In addition, our results may be considered as one model case for examining the effect of large-scale anthropogenic disturbances on the productivity of NTFPs that have similar properties (e.g., clonal reproduction via rhizomes) to S. kurilensis. The findings of this study can also serve as comparative data for NTFPs with properties that are different from S. kurilensis. The findings in this study not only expand our fundamental knowledge of S. kurilensis growth but may also make an important contribution toward future forest management efforts, including considerations for management of NTFPs.

Supporting information

S1 Fig. Map and photos of disturbed areas in each study site in the Teshio Experimental Forest of Hokkaido University.

(a) Disturbed areas in each study site in the Teshio Experimental Forest of Hokkaido University. Solid zones and lines in the above map indicate site locations and woodland paths, respectively. Numbers on the above map indicate the site ID numbers. (b-e) Photos of disturbed areas in (b) study site 1, (c) study site 5, (d) study site 8 and (e) study site 11. Photos were taken in May 2014, which is 2 years before the main survey.

(DOCX)

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S2 Fig. Changes in the number and diameter of old and young bamboo culms after soil scarification.

Blue and red circles indicate the mean value per plot in control and scarification treatments, respectively. Dotted blue lines (P > 0.05) and solid red lines (P < 0.05) denote linear regression in control and scarification treatments, respectively.

(DOCX)

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S1 Table. Number of survey plots at each site.

(DOCX)

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S2 Table. Statistical results of general linear mixed models (GLMMs) about the effects of soil scarification on old and young culms.

(DOCX)

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S3 Table. Statistical results of subsequent general linear mixed models (GLMMs) about the changes in status of old and young culms.

(DOCX)

Click here for additional data file. (21.8KB, docx)
S1 File. Dataset in this study.

(XLSX)

Click here for additional data file. (18.5KB, xlsx)

Acknowledgments

We especially thank Dr. Koya Hashimoto for his helpful comments on our statistical analyses. We are also grateful to the staff at the Teshio Experimental Forest of Hokkaido University for their support during this study.

Data Availability

All relevant data are within the manuscript and its Supporting Information files.

Funding Statement

This work was supported by KAKENHI Grant Number 26740045 (NK) and 18K11724 (NK) from Japan Society for the Promotion of Science. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Decision Letter 0

Xiao Guo

21 Sep 2020

PONE-D-20-20261

Demography and productivity during the recovery time sequence of a wild edible bamboo after large-scale anthropogenic disturbance

PLOS ONE

Dear Dr. Katayama,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Please submit your revised manuscript by Nov 05 2020 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

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We look forward to receiving your revised manuscript.

Kind regards,

Xiao Guo, Ph.D.

Academic Editor

PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: No

Reviewer #2: Yes

**********

2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The growth of bamboo forest is affected by soil condition, mother bamboo condition and bamboo shoot harvest.

In this study, only the soil condition was discussed, and the other two aspects were ignored. The control and treatment groups were quite different in the condition of mother bamboo and bamboo shoot harvest.

As a bamboo shoot forest, its growth status is not only related to soil, but also closely related to human bamboo shoot harvesting behavior.

Generally, bamboo shoot harvesting and soil scarification occur simultaneously in the bamboo forest managed by shoot use forest.

In this paper, the author only considered the soil scarification factors, and did not consider the bamboo shoot harvesting. Bamboo shoot harvesting can affect the growth of bamboo forest more than soil scarification .

This kind of artificially managed bamboo forest is often accompanied by the felling of old bamboo, which affects the quantity of old bamboo and new bamboo, and also leads to the difference of DBH between new bamboo and old bamboo.

In this paper, the number of old bamboos in the treatment group was significantly different from that in the control group.

As a bamboo species with 8-year-old individual bamboo plant, it is of little significance to study the soil scarification for more than 40 years. Unless it is proved that the physical and chemical properties of soil have changed with the increase of soil scarification time, else, the significance of 20 years and 40 years is not significant. This difference is largely caused by the management behavior of harvesting bamboo shoots and cutting down old bamboo.

The sample plot area of bamboo survey is 1 * 1m, which is too small. In China, the investigated area of Moso bamboo forest is not less than 400 square meters, and the number of small bamboo species is not less than 2 * 2 meters.

Generally speaking, the conclusion of this paper is unreasonable.

Reviewer #2: The manuscript is well organized and written. Soil scarification is one important measure for the secondary forest management. However, soil scarification has been conducted to remove dwarf bamboos rather to cultivate them. Therefore, the dwarf bamboos recovering from the field after soil scarification might be influenced greatly by the environment around. Accordingly, the description of experimental site should be more clear to avoid such a confusion. Moreover, the area of survey plot as 1*1 m2 is suitable to demonstrate the demograhpy and productivity of bamboos?

**********

6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

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Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

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PLoS One. 2020 Dec 1;15(12):e0243089. doi: 10.1371/journal.pone.0243089.r002

Author response to Decision Letter 0

1 Oct 2020

[Reviewer #1]

[Comment]

The growth of bamboo forest is affected by soil condition, mother bamboo condition and bamboo shoot harvest. In this study, only the soil condition was discussed, and the other two aspects were ignored. The control and treatment groups were quite different in the condition of mother bamboo and bamboo shoot harvest.

[Response]

Thank you very much for this comment. We understand that these concerns arise due to the lack of explanation of the study sites. Our study was carried out in an experimental forest for research on forestry, not a commercial forest for the harvest bamboos. Therefore, the traffic of people to harvest bamboos was fundamentally restricted. In addition, we carefully selected undisturbed study sites after soil scarification (please see lines 139-140). There was no record of the harvest of young and old bamboo culms in these sites and, therefore, we could ignore the effects of harvesting on the present results. Another concern about the condition of mother bamboos is likely your misunderstanding. The "original" condition of mother bamboos would not differ between treatment groups (i.e., disturbed areas vs. control areas) because "each" control area was located within the study site (100 m from the edge of "each" disturbed area). The paired areas were selected in this manner in order to avoid any confounding effects of environmental conditions (including the state of the mother bamboo). In fact, there were no significant differences in the numbers and stem diameters of old as well as young culms between treatment groups in sites where more than 24 years had passed after soil scarification (please see Figure 1). This indicates that the bamboo stands had recovered by the time of the study (24 years after soil scarification), and the "original" status of mother bamboos was similar between disturbed and control areas in our study. We added this statement in lines 165-176.

[Comment]

As a bamboo shoot forest, its growth status is not only related to soil, but also closely related to human bamboo shoot harvesting behavior.

Generally, bamboo shoot harvesting and soil scarification occur simultaneously in the bamboo forest managed by shoot use forest.

In this paper, the author only considered the soil scarification factors, and did not consider the bamboo shoot harvesting. Bamboo shoot harvesting can affect the growth of bamboo forest more than soil scarification.

[Response]

Please see the response above describing the history and use conditions of the forest of this study site as well as the selection of sampling areas within the forest.

[Comment]

This kind of artificially managed bamboo forest is often accompanied by the felling of old bamboo, which affects the quantity of old bamboo and new bamboo, and also leads to the difference of DBH between new bamboo and old bamboo.

[Response]

First, please understand that our study sites were not set in an artificially managed bamboo forest but in an experimental forest where bamboo succession occurred naturally. As suggested by your comment, litter of old culms would affect the productivity and stem diameter (in similar sense to DBH) of bamboo culms via nutrient cycling. The amount of litter just after soil scarification would be small because soil scarification removes the above- and below-ground tissues of bamboos. Of course, the recovery process includes changes in litter amount, but the main focus in this study is not to evaluate the effects of "individual" processes on the dynamics of bamboos but to demonstrate "overall" demography and productivity after soil scarification. We consider that Figure 1 shows these results, which support this study approach.

[Comment]

In this paper, the number of old bamboos in the treatment group was significantly different from that in the control group.

[Response]

Until 24 years had passed after soil scarification, there were significant differences in the number of old culms between treatment groups (Figure 1) because the bamboo stands were indeed in the recovering process. After that time, there were no significant differences in the number of old culms between treatment groups, indicating that the bamboo stands had recovered completely. Thus, differences are meaningful and not the result of poor study design.

[Comment]

As a bamboo species with 8-year-old individual bamboo plant, it is of little significance to study the soil scarification for more than 40 years. Unless it is proved that the physical and chemical properties of soil have changed with the increase of soil scarification time, else, the significance of 20 years and 40 years is not significant. This difference is largely caused by the management behavior of harvesting bamboo shoots and cutting down old bamboo.

[Response]

We realize that the explanation of life history of the dwarf bamboo (Sasa kurilensis) was insufficient here. The lifespan of an individual "culm" (each ramet) was actually about 8 years, but the individual dwarf bamboo (genet) was longer lived and may have a lifespan of more than 60 years. We added this statement in line 119. In addition, please check again to confirm that we show "no differences" in the number of old and young culms in the sites where more than 24 years had passed after soil scarification (please see Figure 1).

[Comment]

The sample plot area of bamboo survey is 1 * 1m, which is too small. In China, the investigated area of Moso bamboo forest is not less than 400 square meters, and the number of small bamboo species is not less than 2 * 2 meters.

[Response]

Thank you for your comment. Because the Mosso bamboo has a large body (culm) size, a larger sized plot may be necessary to survey the demography. However, our target bamboo was a dwarf bamboo that forms condensed colonies. The number of old culms was frequently over 10 per m2 (average density of old culms in control area was 13.4 culms/m2; please see S2 Fig). As you suggest, the plot size may be a bit small, but even so, we could demonstrate a meaningful recovery process (Figure 1). In addition, please confirm that we prepared at least 4 plots per site. Thus, the demography of the target bamboo was checked in at least a 2×2 meter square area at each site.

[Comment]

Generally speaking, the conclusion of this paper is unreasonable.

[Response]

We appreciate your comment, but we do not understand how to better express this conclusion based on your comment. Thus, we removed the last paragraph (conclusion) from the manuscript.

[Reviewer #2]

[Comment]

The manuscript is well organized and written. Soil scarification is one important measure for the secondary forest management. However, soil scarification has been conducted to remove dwarf bamboos rather to cultivate them. Therefore, the dwarf bamboos recovering from the field after soil scarification might be influenced greatly by the environment around. Accordingly, the description of experimental site should be more clear to avoid such a confusion.

[Response]

Thank you very much for your comment. We apologize for the inadequate explanation of our study sites. In this study, each control area was set adjacent to each corresponding disturbed area in order to ensure similar environmental conditions between the paired areas. Actually, the number and diameter of old culms, which strongly reflect the differences in environmental conditions (N. Katayama, in review), did not differ between treatment groups at more than 24 years after the soil scarification. We added this statement in lines 165-176.

[Comment]

Moreover, the area of survey plot as 1*1 m2 is suitable to demonstrate the demograhpy and productivity of bamboos?

[Response]

Thank you very much for your comment. Please see our response to a similar comment from Reviewer #1.

We hope that our modifications adequately address all of the comments of the reviewers. We hope that the revised manuscript is now acceptable for publication in PLoS ONE.

Attachment

Submitted filename: Response_to_Reviewers.docx

Click here for additional data file. (24.4KB, docx)

Decision Letter 1

Xiao Guo

30 Oct 2020

PONE-D-20-20261R1

Demography and productivity during the recovery time sequence of a wild edible bamboo after large-scale anthropogenic disturbance

PLOS ONE

Dear Dr. Katayama,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Please submit your revised manuscript by Dec 14 2020 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.

  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.

  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: http://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols

We look forward to receiving your revised manuscript.

Kind regards,

Xiao Guo, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (if provided):

The authors have revised the manuscript according to the comments of two reviewers. The manuscript has been greatly improved. However one reviewer still has a concern about the English language of this manuscript. I agree with the reviewer and therefore recommend the manuscript be formally accepted after language polishment. A certificate of English editing by a professional company will be greatly appreciated. 

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: (No Response)

Reviewer #2: In this version, all comments of the original were addressed soundly. Therefore, the manuscript is recommended to be published with a language polishment.

**********

7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step.

PLoS One. 2020 Dec 1;15(12):e0243089. doi: 10.1371/journal.pone.0243089.r004

Author response to Decision Letter 1

10 Nov 2020

[Editor]

The authors have revised the manuscript according to the comments of two reviewers. The manuscript has been greatly improved. However one reviewer still has a concern about the English language of this manuscript. I agree with the reviewer and therefore recommend the manuscript be formally accepted after language polishment. A certificate of English editing by a professional company will be greatly appreciated.

[Reviewer #2]

[Comment]

In this version, all comments of the original were addressed soundly. Therefore, the manuscript is recommended to be published with a language polishment.

[Response]

We are very glad to hear these comments, and we apologize for the poor English. The English in this manuscript was carefully checked again by a science editor before the resubmission of this revision. I hope that the revised manuscript is now acceptable for publication in PLOS ONE.

Thank you again in advance for your kind consideration of this manuscript.

On behalf of the authors,

Noboru Katayama

Attachment

Submitted filename: Response_to_Reviewers.docx

Click here for additional data file. (20.6KB, docx)

Decision Letter 2

Xiao Guo

16 Nov 2020

Demography and productivity during the recovery time sequence of a wild edible bamboo after large-scale anthropogenic disturbance

PONE-D-20-20261R2

Dear Dr. Katayama,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

An invoice for payment will follow shortly after the formal acceptance. To ensure an efficient process, please log into Editorial Manager at http://www.editorialmanager.com/pone/, click the 'Update My Information' link at the top of the page, and double check that your user information is up-to-date. If you have any billing related questions, please contact our Author Billing department directly at authorbilling@plos.org.

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Kind regards,

Xiao Guo, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

The English language in this manuscript has been carefully checked and edited by a science editor. I recommend the manuscript be accepted for publication in its current form in PLoS ONE.

Reviewers' comments:

Acceptance letter

Xiao Guo

18 Nov 2020

PONE-D-20-20261R2

Demography and productivity during the recovery time sequence of a wild edible bamboo after large-scale anthropogenic disturbance

Dear Dr. Katayama:

I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now with our production department.

If your institution or institutions have a press office, please let them know about your upcoming paper now to help maximize its impact. If they'll be preparing press materials, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information please contact onepress@plos.org.

If we can help with anything else, please email us at plosone@plos.org.

Thank you for submitting your work to PLOS ONE and supporting open access.

Kind regards,

PLOS ONE Editorial Office Staff

on behalf of

Dr. Xiao Guo

Academic Editor

PLOS ONE

Associated Data

    This section collects any data citations, data availability statements, or supplementary materials included in this article.

    Supplementary Materials

    S1 Fig. Map and photos of disturbed areas in each study site in the Teshio Experimental Forest of Hokkaido University.

    (a) Disturbed areas in each study site in the Teshio Experimental Forest of Hokkaido University. Solid zones and lines in the above map indicate site locations and woodland paths, respectively. Numbers on the above map indicate the site ID numbers. (b-e) Photos of disturbed areas in (b) study site 1, (c) study site 5, (d) study site 8 and (e) study site 11. Photos were taken in May 2014, which is 2 years before the main survey.

    (DOCX)

    Click here for additional data file. (2.8MB, docx)
    S2 Fig. Changes in the number and diameter of old and young bamboo culms after soil scarification.

    Blue and red circles indicate the mean value per plot in control and scarification treatments, respectively. Dotted blue lines (P > 0.05) and solid red lines (P < 0.05) denote linear regression in control and scarification treatments, respectively.

    (DOCX)

    Click here for additional data file. (142.9KB, docx)
    S1 Table. Number of survey plots at each site.

    (DOCX)

    Click here for additional data file. (22.5KB, docx)
    S2 Table. Statistical results of general linear mixed models (GLMMs) about the effects of soil scarification on old and young culms.

    (DOCX)

    Click here for additional data file. (22.4KB, docx)
    S3 Table. Statistical results of subsequent general linear mixed models (GLMMs) about the changes in status of old and young culms.

    (DOCX)

    Click here for additional data file. (21.8KB, docx)
    S1 File. Dataset in this study.

    (XLSX)

    Click here for additional data file. (18.5KB, xlsx)
    Attachment

    Submitted filename: Response_to_Reviewers.docx

    Click here for additional data file. (24.4KB, docx)
    Attachment

    Submitted filename: Response_to_Reviewers.docx

    Click here for additional data file. (20.6KB, docx)

    Data Availability Statement

    All relevant data are within the manuscript and its Supporting Information files.

    Articles from PLoS ONE are provided here courtesy of PLOS

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