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. 2019 Nov 8;4(2):3890–3891. doi: 10.1080/23802359.2019.1688117

Sequence and phylogenetic analysis of the complete mitochondrial genome for Hepu mitten crab (Eriocheir hepuensis) from Nanjiujiang River basin

Cheng Zhang a,b, Qingqing Li a,b, Qingguo Meng c, Wen Wang c, Yongxu Cheng a,b,d, XuGan Wu a,b,d,✉
PMCID: PMC7707772  PMID: 33366237

Abstract

Taxonomic classification of Eriocheir hepuensis was ambiguous, and it has long been controversial. In this study, the whole mitochondrial genome of E. hepuensis was determined to be 16,397 bp, including 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 control region. A total of 20 intergenic gaps were detected, and the AT content of whole mitochondrial genome was 71.78%. Phylogenetic analysis confirmed that the evolutionary relationship of E. hepuensis, E. sinensis, and E. japonica are most likely to be three species with the same taxonomic status. The whole mitogenome of this species will be useful for the future animal evolutionary, phylogenetic relationship, phylogeny and genomic studies in the genus Eriocheir.

Keywords: Eriocheir hepuensis, mitochondrial genome, taxonomic classification, phylogenetic

Hepu mitten crab (Eriocheir hepuensis) belongs to the genus: Decapoda, Varunidae, Eriocheir. But its higher classifications were ambiguous, and taxonomic boundaries between genera and species, and species and subspecies of E. hepuensis have been controversial (Li and Li 1999; Li and Zou 1999; Tang et al. 2003; Wang et al. 2008). In the present study, specimen of E. hepuensis (Wu, HP7) was collected from November to December 2017 by fishermen in Nanliujiang River basin (109.04°E, 21.39°N), and it was deposited in Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, China. The total genomic DNA was extracted using phenol-chloroform protocol, and the complete mitochondrial genome (mtDNA) was determined by using High-throughput Illumina Sequencing technology, and proofread by Sanger method. The location of protein-coding genes (PCGs), transfer RNA (tRNA) genes and ribosomal RNA (rRNA) genes were identified using the MITOS Web Server (Bernt et al. 2013). The annotated genomic sequence has been submitted to GenBank with the accession number MK159104.

In total, the complete mtDNA of E. hepuensis was 16,397 bp in length. The overall A + T content of the whole mtDNA was 71.78%. The complete mtDNA contained 13 PCGs, 22 tRNA genes, two rRNA genes, and one control region. Among the 37 genes, four PCGs (ND1, ND4, ND4L, and ND5), eight tRNA genes (tRNAPro, tRNALeu(CUN), tRNAHis, tRNAVal, tRNAGln, tRNACys, tRNATyr, and tRNAPhe), and two rRNA genes (12S rRNA, 16S rRNA) were on the light strand, and the remaining 23 genes were on the heavy strand. All 13 PCGs from the complete mtDNA were initiated by the typical start codon ATN (ATA for ND1 and ND6 genes, ATC for ND2 and ND3 genes, ATC for ND3 gene, ATT for ATP6, ATG for ATP8, COX I, COX II, COX III, Cyt b, ND4, ND4L, and ND5 genes and ATT for ND6 gene). The typical termination codons (TAA or TAG) were detected in 10 PCGs (TAA for ATP6, ATP8, COX II, COX III, ND1, ND3, ND4, ND4L, and ND6 genes, TAG for ND2 and ND5 genes). The remaining two genes (COX I and Cyt b) were ended by incomplete stop codons (T––). All tRNA genes, ranged from 63 bp to 73 bp, and a total of 29 base mismatches were detected. Among them, there are one mismatch number of A–A base pairs, two mismatches of A–C base pairs, two mismatches of U–U base pairs, and 24 mismatches of U–G base pairs. The 12S rRNA is 827 bp, while the 16S rRNA is 1336 bp, and they were located between tRNALeu (CUN) and tRNAHis. The 952 bp control region was located in tRNAVal and tRNAGln. A total of 20 intergenic gaps were detected in the complete mtDNA of E. hepuensis, and A + T content of the control region was 83.61%. The phylogenetic relationship was estimated using the maximum likelihood method. The results showed that E. hepuensis, E. sinensis, and E. japonica are most likely to be three species with the same taxonomic status (Figure 1).

Figure 1.

Figure 1.

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The Maximum Likelihood tree inferred from 13 PCGs of nine species. Numbers at the branches indicated the bootstrapping values with 1000 replications. GenBank accession numbers are on the right side of the vertical line. ‘Hollow circle’ represents a sequence in this study.

Disclosure statement

No potential conflict of interest was reported by the authors.

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