Abstract
Auricularia heimuer is one of the most popular edible fungi in China. It has high economic and medicinal value. The complete mitochondrial genome of A. heimuer is 40586 bp. It contains 48 genes, including 25 protein-coding genes, 22 tRNA genes, and 1 gene coding RNase P RNA. The G + C content is 37.92%. The phylogenetic relationships were constructed on the Bayesian inference method. It showed that A. heimuer was in distance to other species of Russulales, Polyporales, and Agaricales. The complete genome sequence of A. heimuer will provide a useful resource for the genetic and systematics studies.
Keywords: Auricularia heimuer, mitochondrial genome, phylogeny, edible mushroom
Auricularia heimuer is one of the most popular edible fungi in China. The fruiting body is ear-like and rich in gelatin. It has a high economic and medicinal value which can effectively reduce the blood lipid level in the human body (Yuan et al. 2019). Since the twenty-first century, with the landmark technology (cultivation with intermittent mist under sunlight) (Ying et al. 2010), the A. heimuer industry has entered a new development stage. Mitochondrial DNA is involved in the regulation of mushroom traits (Hintz et al. 1988), the exploration of A. heimuer mitochondrial genome will be benefit to the research of its genetics and breeding.
The complete mitochondrial genome sequence of A. heimuer was determined. It was sampled from Yanbian, Jilin province, China (128°10′13′′ E42°27′49′′) on decaying stumps. A voucher specimen (No. J2015032202) was deposited in the college of Horticulture of Jilin Agricultural University. Genomic DNA was extracted using a QIAamp DNA Mini Kit (Qiagen, Hilden, Germany). Genome sequencing libraries with 500 bp and 2 kb were constructed for de novo sequencing. The DNA libraries were performed on the Illumina HiSeq 2500 system (Illumina, San Diego, CA, USA). The complete mitochondrial genome was assembled as implemented by SPAdes version 3.9.0 (Anton et al. 2012). MFannot (http://megasun.bch.umontreal.ca/cgi-bin/mfannot/mfannotInterface.pl) and tRNAscan-SE (Chan and Lowe 2019) were used for mitochondrial genome and tRNA annotation.
The total length of complete mitogenome of A. heimuer was 40586 bp (GenBank accession number MN510416). It contains 48 genes, including 25 protein-coding genes, 22 tRNA genes, and 1 gene coding RNase P RNA. The G + C content is 37.92%, the A, T, G, and C account for 31.75, 30.32, 19.26, and 18.66%. The phylogenetic tree was constructed by 14 core protein-coding genes of 22 species from Agaricomycetes. The corresponding sequence of Cantharellus cibarius was used as an outgroup. MAFFT (Katoh and Standley 2013) was used for multiple sequence alignment. ModelFinder (Kalyaanamoorthy et al. 2017) was for optimal partitioning strategy and model selection. The phylogenetic tree was constructed by MrBayes (Ronquist et al. 2012) (Figure 1). The phylogenetic relationships showed that A. heimuer was in distance to other species of Russulales, Polyporales, and Agaricales.
Figure 1.
Phylogenetic tree showing the relationship between A. heimuer and 23 fungi based on Bayesian inference.
Disclosure statement
No potential conflict of interest was reported by the authors.
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