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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Neurotoxicology. 2020 Sep 28;81:116–126. doi: 10.1016/j.neuro.2020.09.037

Figure 12. Representative tracing of fura-2 fluorescence ratio F(340/380) from a hiPSC-MN during exposure to 1.5 μM MeHg + 20 μM EGTA.

Figure 12.

The initial peak represents increase in Ca2+ as a result of depolarization of the cell after 1 min exposure to 40 mM KCl. Subsequent exposure to 1.5 μM MeHg + 20 μM EGTA-containing HBS (Ca2+ free HBS) removed the 2nd phase normally observed with MeHg exposure. This demonstrates that the 2nd phase is dependent on the influx of extracellular Ca2+.