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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Neurotoxicology. 2020 Sep 28;81:116–126. doi: 10.1016/j.neuro.2020.09.037

Figure 3. Expression of NFH, GluA2 and GluA1 subunit on hiPSC-MNs after 7 DIV.

Figure 3.

DIV 7 hiPSC were fixed and immunolabeling was performed using chicken anti-NFH primary antibody and goat anti chicken Alexa Fluor 594 secondary antibody (A). Rabbit anti-GluA2 primary antibody conjugated with Alexa Fluor 488 secondary antibody was used (B). Rabbit anti-GluA1 and goat anti-rabbit Alexa Fluor 405 secondary antibody was used (C). Merged images of NFH, GluA1, and GluA2 showed these subunits in our hiPSC-MN cultures. Images were taken at 20x magnification with 2x zoom from the same field of cells. Scale bar = 50 μm.