Fig. 5.

NHR-23 is necessary for sperm development. (A) DIC images of the proximal germlines of mex-5p::TIR1, nhr-23::GFP::AID*::3xFLAG young adult males grown from L1 onwards on control or 4 mM auxin media. (B) DIC images of wild-type and masculinized mex-5p::TIR1, nhr-23::GFP::AID*::3xFLAG; fbf-1(ok91) fbf-2(q704) hermaphrodites grown from L1 onwards on control or 4 mM auxin media. (1) Primary spermatocytes have a flat morphology with a large nucleus. (2) Residual bodies appear as highly refractile raised button-like structures. (3) Spermatids are readily discernible through their characteristic small refractive nuclei. (C) DIC image of the proximal germline in a wild-type young adult male F1 progeny from a P₀ animal injected with a mex-5p::nhr-23 cDNA co-suppression construct, which promotes an RNAi-like depletion of targeted mRNA. Scale bars: 40 µm.